Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot

doi:10.1016/S2095-3119(14)60800-5

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (4): 691-697.DOI: 10.1016/S2095-3119(14)60800-5

Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot

WANG Qing, ZUO Jin-hua, WANG Qian, NA Yang, GAO Li-pu

Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Fruits and Vegetable Storage and Processing/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture/Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing 100097, P.R.China

收稿日期:2014-03-31 出版日期:2015-04-01 发布日期:2015-04-10
通讯作者: GAO Li-pu, Tel/Fax: +86-10-51503051,E-mail: gaolipu@nercv.org
作者简介:WANG Qing, E-mail: wangqing@nercv.org;
基金资助:
This research was supported by grants from the National Natural Science Foundation of China (31101364), the Ministry of Agriculture of China (CARS-25-E-01 and 201203095) and the Beijing Academy of Agriculture and Forestry Sciences, China (CXJJ201304).

Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot

WANG Qing, ZUO Jin-hua, WANG Qian, NA Yang, GAO Li-pu

Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Fruits and Vegetable Storage and Processing/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture/Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing 100097, P.R.China

Received:2014-03-31 Online:2015-04-01 Published:2015-04-10
Contact: GAO Li-pu, Tel/Fax: +86-10-51503051,E-mail: gaolipu@nercv.org
About author:WANG Qing, E-mail: wangqing@nercv.org;
Supported by:
This research was supported by grants from the National Natural Science Foundation of China (31101364), the Ministry of Agriculture of China (CARS-25-E-01 and 201203095) and the Beijing Academy of Agriculture and Forestry Sciences, China (CXJJ201304).

摘要/Abstract

摘要： The antifungal activity of chitosan on a common fungal phytopathogen, Sclerotinia sclerotiorum, and the control effect on sclerotinia rot of carrot were investigated. Mycelial growth and fungal biomass were strongly inhibited by chitosan. Using propidium iodide stain combined with fluorescent microscopy, the plasma membrane of chitosan-treated S. sclerotiorum mycelia was observed to be markedly damaged. Concomitantly, protein leakage and lipid peroxidation was also found to be significantly higher in chitosan-treated mycelia compared to the control. Chitosan provided an effective control of sclerotinia rot of carrot, with induction of activity of defense-related enzymes including polyphenoloxidase and peroxidase. These data suggest that the effects of chitosan on sclerotinia rot of carrot may be associated with the direct damage to the plasma membrane and lipid peroxidation of S. sclerotiorum, and the elicitation of defense response in carrot.

关键词: antifungal activity , carrot , chitosan , plasma membrane , Sclerotinia sclerotiorum

Abstract: The antifungal activity of chitosan on a common fungal phytopathogen, Sclerotinia sclerotiorum, and the control effect on sclerotinia rot of carrot were investigated. Mycelial growth and fungal biomass were strongly inhibited by chitosan. Using propidium iodide stain combined with fluorescent microscopy, the plasma membrane of chitosan-treated S. sclerotiorum mycelia was observed to be markedly damaged. Concomitantly, protein leakage and lipid peroxidation was also found to be significantly higher in chitosan-treated mycelia compared to the control. Chitosan provided an effective control of sclerotinia rot of carrot, with induction of activity of defense-related enzymes including polyphenoloxidase and peroxidase. These data suggest that the effects of chitosan on sclerotinia rot of carrot may be associated with the direct damage to the plasma membrane and lipid peroxidation of S. sclerotiorum, and the elicitation of defense response in carrot.

Key words: antifungal activity , carrot , chitosan , plasma membrane , Sclerotinia sclerotiorum

WANG Qing, ZUO Jin-hua, WANG Qian, NA Yang, GAO Li-pu. Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot[J]. Journal of Integrative Agriculture, 2015, 14(4): 691-697.

参考文献

Bautista-Baños S, Hernández-Lauzardo A N, Velázquez-delValle M G, Hernández-López M, Ait Barka E, Bosquez-Molina E, Wilson C L. 2006. Chitosan as a potential naturalcompound to control pre and post harvest diseases ofhorticultural commodities. Crop Protection, 25, 108-118

Boland G J, Hall R. 1994. Index of plant hosts of Sclerotiniasclerotiorum. Canadian Journal of Plant Pathology, 16,93-108

Bradford M N. 1976. A rapid and sensitive method for thequantitation of microgram quantities of protein using theprinciple of protein-dye binding. Analytical Biochemistry,72, 248-254

Chailoo M J, Asghari M R. 2011. Hot water and chitosantreatment for the control of postharvest decay in sweetcherry (Prunus avium L.) cv. Napoleon (Napolyon). Journalof Stored Products Research, 2, 135-138

Cheah L H, Page B B C, Shepherdb R. 1997 Chitosan coatingfor inhibition of sclerotinia rot of carrots. New ZealandJournal of Crop and Horticultural Science, 25, 89-92

Chen C, Bélanger R R, Benhamou N, Paulitz T C. 2000.Defense enzymes induced in cucumber roots by treatmentwith plant growth-promoting rhizobacteria (PGPR) andPythium aphanidermatum. Physiological and MolecularPlant Pathology, 56, 13-23

Chien P J, Chou C C. 2006. Antifungal activity of chitosan and itsapplication to control postharvest quality and fungal rottingof Tankan citrus fruit (Citrus tankan hayata). Journal of theScience of Food and Agriculture, 86, 1964-1969

Cota-Arriola O, Cortez-Rocha M O, Rosas-Burgos E C, Burgos-Hernández A, López-Franco Y L, Plascencia-JatomeaM. 2011. Antifungal effect of chitosan on the growth ofAspergillus parasiticus and production of aflatoxin B1.Polymer International, 60, 937-944

El-Mougy N S, Abdel-Kader M M, Aly M H. 2012. Effect of a newchemical formula on postharvest decay incidence in citrusfruit. Journal of Plant Protection Research, 52, 156-164

Eweis M, Elkholy S S, Elsabee M Z. 2006. Antifungal efficacyof chitosan and its thiourea derivatives upon the growthof some sugar-beet pathogens. International Journal ofBiological Macromolecules, 38, 1-8

Hernández-Lauzardo A N, Bautista-Banõs S, Velázquez-delValle M G, Méndez-Montealvo M G, Sánchez-Rivera MM, Bello-Pérez L A. 2008. Antifungal effects of chitosanwith different molecular weights on in vitro developmentof Rhizopus stolonifer (Ehrenb.:Fr.) Vuill. CarbohydratePolymers, 73, 541-547

Ippolito A, El Ghaouth A, Wilson C L, Wisniewski M. 2000.Control of postharvest decay of apple fruit by Aureobasidiumpullulans and induction of defense responses. PostharvestBiology and Technology, 19, 265-272

Kora C, McDonald M R, Boland G J. 2003. Sclerotinia rot ofcarrot: An example of phenological adaptation and bicyclicdevelopment of Sclerotinia sclerotiorum. Plant Disease,87, 456-470

Kushwaha S K S, Rai A K, Singh S. 2010. Chitosan: aplatform for targeted drug delivery. International Journal ofPharmTech Research, 2, 2271-2282

Laflamme P, Benhamou N, Bussières G, Dessureault M. 1999.Differential effect of chitosan on root rot fungal pathogensin forest nurseries. Canadian Journal of Botany, 77,1460-1468

Liu J, Tian S, Meng X, Xu Y. 2007. Effects of chitosan on controlof postharvest diseases and physiological responses oftomato fruit. Postharvest Biology and Technology, 11,131-140

Liu J, Zong Y, Qin G, Li B, Tian S. 2010. Plasma membranedamage contributes to antifungal activity of silicon againstPenicillium digitatum. Current Microbiology, 61, 274-279

Lopez-Diez E C, Bone S. 2000. An investigation of the waterbindingproperties of protein+sugar systems. Physics inMedicine and Biology, 45, 3577-3588

Meng X, Yang L, Kennedy J F, Tian S P. 2010. Effectsof chitosan and oligochitosan on growth of two fungalpathogens and physiological properties in pear fruit.Carbohydrate Polymers, 81, 70-75

Molloya C, Cheah L H, Koolaard J P. 2004 Induced resistanceagainst S. sclerotiorum in carrots treated with enzymaticallyhydrolysed chitosan. Postharvest Biology and Technology,33, 61-65

Ojaghian M R, Almoneafy A A, Cui Z Q, Xie G L, Zhang J,Shang C, Li B. 2013 Application of acetyl salicylic acid andchemically different chitosans against storage carrot rot.Postharvest Biology and Technology, 84, 51-60

Pacheco N, Larralde-Coron C P, Sepulveda J, Trombottoc S,Domardc A, Shirai K. 2008. Evaluation of chitosans and Pichiaguillermondii as growth inhibitors of Penicillium digitatum.International Journal of Biological Macromolecules, 43,20-26

Reddy M V B, Angers P, Castaigne F, Arul J. 2000. Chitosaneffects on blackmold rot and pathogenic factors produced byAlternaria alternata in postharvest tomatoes. Journal of theAmerican Society for Horticultural Science, 125, 742-747

Reddy M V B, Arul J, Ait-Barka E, Castaigne F, Arul J. 1997.Effect of chitosan on growth and toxin production byAlternaria alternata f. sp. lycopersici. HortScience, 32,467-468

Ritter A, Goulitquer S, Salaün J P, Tonon T, Correa J A, Potin P.2008. Copper stress induces biosynthesis of octadecanoidand eicosanoid oxygenated derivatives in the brown algalkelp Laminaria digitata. New Phytologist, 180, 809-821

Terry L A, Joyce D C. 2004. Elicitors of induced diseaseresistance in postharvest horticultural crops: a brief review.Postharvest Biology and Technology, 32, 1-13

Wei M K, Wu Q P, Huang Q, Wu J L, Zhang J M. 2008. Plasmamembrane damage to Candida albicans caused by chlorinedioxide (ClO2). Letters in Applied Microbiology, 47, 67-73

Xu J G, Zhao X M, Han X W, Du Y G. 2007. Antifungal activityof oligochitosan against Phytophthora capsici and otherplant pathogenic fungi in vitro. Pesticide Biochemistry andPhysiology, 87, 220-228

Yao H J, Tian S P. 2005. Effects of a biocontrol agent andmethyl jasmonate on postharvest diseases of peach fruitand the possible mechanisms involved. Journal of AppliedMicrobiology, 98, 941-950

Zeng W, Wang D, Kirk W, Hao J. 2012. Use of Coniothyriumminitans and other microorganisms for reducing Sclerotiniasclerotiorum. Biological Control, 60, 225-232

[1]	WEI Fang, HU Jie, YANG Yan, HAO Zhi-da, WU Rui-hua, TIAN Bao-ming, CAO Gang-qiang, ZANG Xin. Transgenic Arabidopsis thaliana expressing a wheat oxalate oxidase exhibits hydrogen peroxide related defense response[J]. Journal of Integrative Agriculture, 2015, 14(12): 2565-2573.
[2]	ZHANG Zi-kun, LI Hua, HE Hong-jun , LIU Shi-qi. Grafting Raises the Cu Tolerance of Cucumber Through Protecting Roots Against Oxidative Stress Induced by Cu Stress[J]. Journal of Integrative Agriculture, 2013, 12(5): 815-824.
[3]	ZHAO Te, GAO Fei, ZHOU Lin, SONG Tian-you. Essential Oil from Inula britannica Extraction with SF-CO2 and Its Antifungal Activity[J]. Journal of Integrative Agriculture, 2013, 12(10): 1791-1798.

Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot

Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics