Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1719-1726.DOI: 10.1016/S2095-3119(13)60695-4

• 论文 • 上一篇    下一篇

Isolation, Identification, and Herbicidal Activity of Metabolites Produced by Pseudomonas aeruginosa CB-4

 YANG Juan, CAO Hong-zhe, WANG Wei, ZHANG Li-hui , DONG Jin-gao   

  1. Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, P.R.China
  • 收稿日期:2013-10-18 出版日期:2014-08-01 发布日期:2014-08-02
  • 通讯作者: ZHANG Li-hui, Tel/Fax: +86-312-7528173, E-mail: zhanglihui@hebau.edu.cn; DONG Jin-gao, Tel/Fax: +86-312-7528266, E-mail: dongjingao@126.com
  • 作者简介:YANG Juan, E-mail: yangjuan018@126.com
  • 基金资助:

    This research was supported by grants from the National High Technology Research and Development Program of China (2011AA10A206) and the Science and Technology Support Program of Hebei, China (20121124001).

Isolation, Identification, and Herbicidal Activity of Metabolites Produced by Pseudomonas aeruginosa CB-4

 YANG Juan, CAO Hong-zhe, WANG Wei, ZHANG Li-hui , DONG Jin-gao   

  1. Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, P.R.China
  • Received:2013-10-18 Online:2014-08-01 Published:2014-08-02
  • Contact: ZHANG Li-hui, Tel/Fax: +86-312-7528173, E-mail: zhanglihui@hebau.edu.cn; DONG Jin-gao, Tel/Fax: +86-312-7528266, E-mail: dongjingao@126.com
  • About author:YANG Juan, E-mail: yangjuan018@126.com
  • Supported by:

    This research was supported by grants from the National High Technology Research and Development Program of China (2011AA10A206) and the Science and Technology Support Program of Hebei, China (20121124001).

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摘要： CB-4, a bacterial strain with highly effective herbicidal activity, was isolated from infected corn leaves. Through morphology, physiological and biochemical tests, and 16S ribosomal DNA gene sequencing methods, CB-4 was identified as Pseudomonas aeruginosa. We conducted activity-evaluation experiments in the laboratory to assess the herbicidal potential of metabolites produced by strain CB-4. Crude extracts of strain CB-4 have high inhibition activity on Digitaria sanguinalis. In general, the root and shoot growth parameters of D. sanguinalis were significantly reduced by metabolites of strain CB-4. The IC50 of the culture filtrate extracts for the radicula and coleoptile of D. sanguinalis were 0.299 and 0.210 mg mL-1, respectively. Component 2 of the herbicidal activity of the crude toxin from strain CB-4 was successfully purified for the first time by using high-speed counter current chromatography with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (4:5:4:5, v/v) and high-performance liquid chromatography. We concluded that the metabolites of strain CB-4 have the potential to be developed as a microbe-based herbicide.

关键词: Pseudomonas aeruginosa , bioherbicide , biocontrol , HSCCC , HPLC

Abstract: CB-4, a bacterial strain with highly effective herbicidal activity, was isolated from infected corn leaves. Through morphology, physiological and biochemical tests, and 16S ribosomal DNA gene sequencing methods, CB-4 was identified as Pseudomonas aeruginosa. We conducted activity-evaluation experiments in the laboratory to assess the herbicidal potential of metabolites produced by strain CB-4. Crude extracts of strain CB-4 have high inhibition activity on Digitaria sanguinalis. In general, the root and shoot growth parameters of D. sanguinalis were significantly reduced by metabolites of strain CB-4. The IC50 of the culture filtrate extracts for the radicula and coleoptile of D. sanguinalis were 0.299 and 0.210 mg mL-1, respectively. Component 2 of the herbicidal activity of the crude toxin from strain CB-4 was successfully purified for the first time by using high-speed counter current chromatography with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (4:5:4:5, v/v) and high-performance liquid chromatography. We concluded that the metabolites of strain CB-4 have the potential to be developed as a microbe-based herbicide.

Key words: Pseudomonas aeruginosa , bioherbicide , biocontrol , HSCCC , HPLC