Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress

doi:10.1016/S2095-3119(13)60578-X

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (6): 1250-1257.DOI: 10.1016/S2095-3119(13)60578-X

Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress

ZHANG Shuai, LI Jing, LÜ Li-min, WANG Chun-yi, LUO Jun-yu , CUI Jin-jie

State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China

收稿日期:2013-03-22 出版日期:2014-06-01 发布日期:2014-06-06
通讯作者: CUI Jin-jie, Tel: +86-372-2562217, Fax: +86-372-2562296, E-mail: cuijinjie@126.com
作者简介:ZHANG Shuai, E-mail: zhsjwl@163.com
基金资助:
This work was supported by the National Natural Science Foundation of China (31201518).

Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress

ZHANG Shuai, LI Jing, LÜ Li-min, WANG Chun-yi, LUO Jun-yu , CUI Jin-jie

State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China

Received:2013-03-22 Online:2014-06-01 Published:2014-06-06
Contact: CUI Jin-jie, Tel: +86-372-2562217, Fax: +86-372-2562296, E-mail: cuijinjie@126.com
About author:ZHANG Shuai, E-mail: zhsjwl@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31201518).

摘要/Abstract

摘要： Plants reshape their transcriptomes, proteomes and metabolomes in response to insect damage. In this study, we used suppression subtractive hybridization to investigate the transcriptomes of two cotton varieties (CCRI41 and CCRI23) under Apolygus lucorum damage. From the CCRI23 libraries we obtained 92 transcripts and from the CCRI41 libraries we obtained 96 transcripts. 26 and 63 of the transcripts from CCRI23 and CCRI41, respectively, had known functions. Using reverse transcription PCR, we detected expression profile of genes with known functions. Ultimately, we identified eight significantly regulated genes, including one downregulated and four upregulated genes from the CCRI41 libraries, and one downregulated and two upregulated genes from the CCRI23 libraries. Only the gene encoding the polyphenol oxidase (PPO) is involved in plant defense against insect herbivores, and the others are related to improving tolerance to insect damage. Quantitative real-time PCR was used to study changes in expression levels during A. lucorum damage in CCRI23 and CCRI41. Significantly regulated genes from CCRI23 showed a response in CCRI23 but not response in CCRI41. Similarly, significantly regulated genes from CCRI41 showed a response in CCRI41 but not response in CCRI23. The results showed that, among transcriptomes of cotton varieties, there are different responses to A. lucorum damage.

关键词: suppression subtractive hybridization , Apolygus lucorum , defense against insect herbivores

Abstract: Plants reshape their transcriptomes, proteomes and metabolomes in response to insect damage. In this study, we used suppression subtractive hybridization to investigate the transcriptomes of two cotton varieties (CCRI41 and CCRI23) under Apolygus lucorum damage. From the CCRI23 libraries we obtained 92 transcripts and from the CCRI41 libraries we obtained 96 transcripts. 26 and 63 of the transcripts from CCRI23 and CCRI41, respectively, had known functions. Using reverse transcription PCR, we detected expression profile of genes with known functions. Ultimately, we identified eight significantly regulated genes, including one downregulated and four upregulated genes from the CCRI41 libraries, and one downregulated and two upregulated genes from the CCRI23 libraries. Only the gene encoding the polyphenol oxidase (PPO) is involved in plant defense against insect herbivores, and the others are related to improving tolerance to insect damage. Quantitative real-time PCR was used to study changes in expression levels during A. lucorum damage in CCRI23 and CCRI41. Significantly regulated genes from CCRI23 showed a response in CCRI23 but not response in CCRI41. Similarly, significantly regulated genes from CCRI41 showed a response in CCRI41 but not response in CCRI23. The results showed that, among transcriptomes of cotton varieties, there are different responses to A. lucorum damage.

Key words: suppression subtractive hybridization , Apolygus lucorum , defense against insect herbivores

ZHANG Shuai, LI Jing, Lü Li-min, WANG Chun-yi, LUO Jun-yu , CUI Jin-jie. Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress[J]. Journal of Integrative Agriculture, 2014, 13(6): 1250-1257.

参考文献

Catala R, Medina J, Salinas J. 2011. Integration of low temperature and light signaling during cold acclimation response in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 108, 16475-16480

Cho E K, Choi Y J. 2009. A nuclear-localized HSP70 confers thermoprotective activity and drought-stress tolerance on plants. Biotechnology Letters, 31, 597-606

Conesa A, Gotz S, Garcia-Gomez J M, Terol J, Talon M, Robles M. 2005. Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics, 21, 3674-3676

Constabel C P, Ryan C A. 1998. A survey of wound- and methyl jasmonate-induced leaf polyphenol oxidase in crop plants. Phytochemistry, 47, 507-511

Deng P, Chen L J, Zhang Z L, Lin K J, Ma W H. 2013. Responses of detoxifying, antioxidant and digestive enzyme activities to host shift of Bemisia tabaci (Hemiptera: Aleyrodidae). Journal of Integrative Agriculture, 12, 296-304

Diatchenko L, Lau Y F, Campbell A P, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov E D, Siebert P D. 1996. Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proceedings of the National Academy of Sciences of the United States of America, 93, 6025-6030

Futuyma D J, Agrawal A A. 2009. Macroevolution and the biological diversity of plants and herbivores. Proceedings of the National Academy of Sciences of the United States of America, 106, 18054-18061

Guo X M, Li F G, Guo S D, Zhang Y S, Liu J H, Yao J B, Li G Y, Zhang C J, Yang Y X, Wang Y. 2010. Bivalent insect- resistant gene transgenic cotton variety CCRI 41 with high efficiency and broad aadaptability. Agricultural Sciences in China, 9, http://www.sciencedirect.com/science/article/ pii/S1671292710603136#

Heidel A J, Baldwin I T. 2004. Microarray analysis of salicylic acid- and jasmonic acid-signalling in responses of Nicotiana attenuata to attack by insects from multiple feeding guilds. Plant Cell and Environment, 27, 1362- 1373.

Hernandez J A, Rubio M, Olmos E, Ros-Barcelo A, Martinez- Gomez P. 2004. Oxidative stress induced by long-term plum pox virus infection in peach (Prunus persica). Physiologia Plantarum, 122, 486-495

Hua H, Lu Q, Cai M, Xu C, Zhou D X, Li X, Zhang Q. 2007. Analysis of rice genes induced by striped stemborer (Chilo suppressalis) attack identified a promoter fragment highly specifically responsive to insect feeding. Plant Molecular Biology, 65, 519-530

Iida H, Kitamura T, Honda K 2009. Comparison of egg- hatching rate, survival rate and development time of the immature stage between B- and Q-biotypes of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) on various agricultural crops. Applied Entomology and Zoology, 44, 267-273

Kessler A, Baldwin I T. 2002. Plant responses to insect herbivory: The emerging molecular analysis. Annual Review of Plant Biology, 53, 299-328

Lau O S, Deng X W. 2012. The photomorphogenic repressors COP1 and DET1: 20 years later. Trends in Plant Science, 17, 584-593

Liu Z F, Yan H C, Wang K B, Kuang T Y, Zhang J P, Gui L L, An X M, Chang W R. 2004. Crystal structure of spinach major light-harvesting complex at 2.72 angstrom resolution. Nature, 428, 287-292

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods, 25, 402-408

Lu Y H, Qiu F, Feng H Q, Li H B, Yang Z C, Wyckhuys K A G, Wu K M. 2008. Species composition and seasonal abundance of pestiferous plant bugs (Hemiptera: Miridae) on Bt Cotton in China. Crop Protection, 27, 465-472

Lu Y H, Wu K M, Guo Y Y. 2007. Flight potential of Lygus lucorum (Meyer-Dur) (Heteroptera: Miridae). Environmental Entomology, 36, 1007-1013

Mahanil S, Attajarusit J, Stout M J, Thipyapong P. 2008. Overexpression of tomato polyphenol oxidase increases resistance to common cutworm. Plant Science, 174, 456- 466.

Mewis I, Tokuhisa J G, Schultz J C, Appel H M, Ulrichs C, Gershenzon J. 2006. Gene expression and glucosinolate accumulation in Arabidopsis thaliana in response to generalist and specialist herbivores of different feeding guilds and the role of defense signaling pathways. Phytochemistry, 67, 2450-2462

Morris E R, Walker J C. 2003. Receptor-like protein kinases: The keys to response. Current Opinion in Plant Biology, 6, 339-342

Nibouche S, Faure G, Kleene P, Ouedraogo S. 1998. First step towards integrated pest management on cotton in Burkina Faso. Crop Protection, 17, 697-701

Reymond P, Weber H, Damond M, Farmer E E. 2000. Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. The Plant Cell, 12, 707-719

Sun Y, Zhang Y J, Cao G C, Gu S H, Wu K M, Gao X W, Guo Y Y. 2011. Rice gene expression profiles responding to larval feeding of the striped stem borer at the 1st to 2nd instar stage. Insect Science, 18, 273-281

Tanaka N, Itoh H, Sentoku N, Kojima M, Sakakibara H, Izawa T, Itoh J I, Nagato Y. 2011. The COP1 ortholog PPS regulates the juvenile-adult and vegetative-reproductive phase changes in rice. The Plant Cell, 23, 2143-2154

Tu L L, Zhang X L, Liu D Q, Jin S X, Cao J L, Zhu L F, Deng F L, Tan J F, Zhang C B. 2007. Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis. Chinese Science Bulletin, 52, 3110-3117

Walling L L. 2000. The myriad plant responses to herbivores. Journal of Plant Growth Regulation, 19, 195-216

Wang J, Constabel C P. 2004. Polyphenol oxidase overexpression in transgenic Populus enhances resistance to herbivory by forest tent caterpillar (Malacosoma disstria). Planta, 220, 87-96

Xu J, Duan X, Yang J, Beeching J R, Zhang P. 2013. Enhanced reactive oxygen species scavenging by overproduction of superoxide dismutase and catalase delays postharvest physiological deterioration of cassava storage roots. Plant Physiology, 161, 1517-1528

Yang H M, Xie S X, Wang L, Jing S L, Zhu X P, Li X W, Zeng W, Yuan H Y. 2011. Identification of up-regulated genes in tea leaves under mild infestation of green leafhopper. Scientia Horticulturae, 130, 476-481

Zhang F, Zhu L, He G C. 2004. Differential gene expression in response to brown planthopper feeding in rice. Journal of Plant Physiology, 161, 53-62

Zhang S A, Zhang Y J, Su H H, Gao X W, Guo Y Y. 2009. Identification and expression pattern of putative odorant- binding proteins and chemosensory proteins in antennae of the microplitis mediator (Hymenoptera: Braconidae). Chemical Senses, 34, 503-512

[1]	GAO Feng, NIU Yi-ding, HAO Jin-feng, BADE Rengui, ZHANG Li-quan , HASI Agula. Identification of Differentially Expressed Genes During Ethylene Climacteric of Melon Fruit by Suppression Subtractive Hybridization[J]. Journal of Integrative Agriculture, 2013, 12(8): 1431-1440.
[2]	CHEN Wei, ZHAI Hong, YANG Yuan-jun, HE Shao-zhen, LIU De-gao , LIU Qing-chang. Identification of Differentially Expressed Genes in Sweetpotato Storage Roots Between Kokei No. 14 and Its Mutant Nongdafu 14 Using PCR-Based cDNA Subtraction[J]. Journal of Integrative Agriculture, 2013, 12(4): 589-595.
[3]	ZHOU Hong, MA Zhe, YUAN Jin , FAN Hong-jie. Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus[J]. Journal of Integrative Agriculture, 2013, 12(2): 327-333.
[4]	XIANG Fei-yu, ZHOU Yong-zhi, ZHOU Jin-lin. Identification of Differentially Expressed Genes in the Salivary Gand of Rhipicephalus haemaphysaloides by the Suppression Subtractive Hybridization Approach[J]. Journal of Integrative Agriculture, 2012, 12(9): 1528-1536.
[5]	LI Liang, WANG Wei-qi, HAN Tian-fu, HOU Wen-sheng. Construction of Two Suppression Subtractive Hybridization Libraries and Identification of Salt-Induced Genes in Soybean[J]. Journal of Integrative Agriculture, 2012, 12(7): 1075-1085.
[6]	PANG Rui-hua, MA Guang-ying, LOU Xue-yuan, BAO Man-zhu , YE Yao-mei. Construction and Analysis of Suppression Subtractive cDNA Library from Fertile Disk Floret Buds of Zinnia elegans [J]. Journal of Integrative Agriculture, 2012, 12(4): 567-575.

Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress

Suppression Subtractive Hybridization Reveals Different Responses of Two Varieties of Gossypium arboreum L. Under Apolygus lucorum Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics