Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus

doi:10.1016/S2095-3119(13)60232-4

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (2): 327-333.DOI: 10.1016/S2095-3119(13)60232-4

Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus

ZHOU Hong, MA Zhe, YUAN Jin , FAN Hong-jie

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China

收稿日期:2011-09-18 出版日期:2013-02-01 发布日期:2013-02-06
通讯作者: Correspondence FAN Hong-jie, Tel: +86-25-84395328, E-mail: fhj@njau.edu.cn
基金资助:
This study was supported by the Program for New Century Excellent Talents (NCET) at the University of China (NCET-08-0794), the National Transgenic Major Program (2009ZX08009-154B), the Key Technology Program (R65286D), Jiangsu Province Science and Technology Support Program (BE2009388), the Fundamental Research Funds for the Central Universities, China (KYT 201003), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China.

Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus

ZHOU Hong, MA Zhe, YUAN Jin , FAN Hong-jie

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China

Received:2011-09-18 Online:2013-02-01 Published:2013-02-06
Contact: Correspondence FAN Hong-jie, Tel: +86-25-84395328, E-mail: fhj@njau.edu.cn
Supported by:
This study was supported by the Program for New Century Excellent Talents (NCET) at the University of China (NCET-08-0794), the National Transgenic Major Program (2009ZX08009-154B), the Key Technology Program (R65286D), Jiangsu Province Science and Technology Support Program (BE2009388), the Fundamental Research Funds for the Central Universities, China (KYT 201003), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China.

摘要/Abstract

摘要： Suppression subtractive hybridization (SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ssp. zooepidemicus (SEZ). There were fourteen genomic regions that only presented in virulent strain ATCC35246. These regions encoded 14 proteins, some of them were homologous to proteins associated with cellular surface structure, molecular synthesis, energy metabolism, regulation, transport systems, and other unknown functions. Primers for 6 particular regions were designed from the already published SEZ sequence. Then, we used PCR to evaluate the distribution and conservation of these 6 DNA fragments in various SEZ strains collected from different sources, regions, groups, and times. The results showed that these 6 DNA fragments were widely distributed in SEZ strains, yet they were not existence in the avirulent strain ST171. Moreover, these fragments could not be detected in other Streptococcus groups.

关键词: Streptococcus equi ssp. zooepidemicus , suppression subtractive hybridization , putative virulent genes , PCR detection

Abstract: Suppression subtractive hybridization (SSH) was performed with virulent strain ATCC35246 and avirulent strain ST171 to identify novel genes associated with virulence in Streptococcus equi ssp. zooepidemicus (SEZ). There were fourteen genomic regions that only presented in virulent strain ATCC35246. These regions encoded 14 proteins, some of them were homologous to proteins associated with cellular surface structure, molecular synthesis, energy metabolism, regulation, transport systems, and other unknown functions. Primers for 6 particular regions were designed from the already published SEZ sequence. Then, we used PCR to evaluate the distribution and conservation of these 6 DNA fragments in various SEZ strains collected from different sources, regions, groups, and times. The results showed that these 6 DNA fragments were widely distributed in SEZ strains, yet they were not existence in the avirulent strain ST171. Moreover, these fragments could not be detected in other Streptococcus groups.

Key words: Streptococcus equi ssp. zooepidemicus , suppression subtractive hybridization , putative virulent genes , PCR detection

ZHOU Hong, MA Zhe, YUAN Jin , FAN Hong-jie. Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus[J]. Journal of Integrative Agriculture, 2013, 12(2): 327-333.

参考文献

[1]Akineden O, Hassan A A, Alber J, El-Sayed A,Estoepangestie A T, Lammler C, Weiss R, Siebert U.2005. Phenotypic and genotypic properties ofStreptococcus equi subsp. zooepidemicus isolatedfrom harbor seals (Phoca vitulina) from the GermanNorth Sea during the phocine distemper outbreak in2002. Veterinary Microbiology, 110, 147-152

[2]Bayer A S, Coulter S N, Stover C K, Schwan W R. 1999.Impact of the high-affinity proline permease gene (putP)on the virulence of Staphylococcus aureus inexperimental endocarditis. Infection and Immunity, 67,740-744

[3]Britta J, Ulrich D, Jörg H, Gabriele B-O. 2001. A subtractivehybridisation analysis of genomic differences betweenthe uropathogenic E. coli strain 536 and the E. coli K-12 strain MG1655 FEMS Microbiology Letters, 199,61-66

[4]Causey R C, Weber J A, Emmans E E, Stephenson L A,Homola A D, Knapp K R, Crowley I F, Pelletier D C,Wooley N A. 2006. The equine immune response toStreptococcus equi subspecies zooepidemicus duringuterine infection. The Veterinary Journal, 172, 248-257

[5]Hong K. 2005. Identification and characterization of a novelfibronectin-binding protein gene from Streptococcusequi subspecies zooepidemicus strain VTU211. FEMSImmunology & Medical Microbiology, 45, 231-237

[6]Jadoun J, Ozeri V, Burstein E, Skutelsky E, Hanski E, Sela S.1998. Protein F1 is required for efficient entry ofStreptococcus pyogenes into epithelial cells. Journalof Infectious Diseases, 178, 147-158

[7]Jiang H, Fan H J, Lu C P. 2009. Identification and distributionof putative virulent genes in strains of Streptococcussuis serotype 2. Veterinary Microbiology, 133, 309-316

[8]Jonsson H, Lindmark H, Guss B. 1995. A protein G-relatedcell surface protein in Streptococcus zooepidemicus.Infection and Immunity, 63, 2968-2975

[9]Korman T M, Boers A, Gooding T M, Curtis N, VisvanathanK. 2004. Fatal case of toxic shock-like syndrome due togroup C streptococcus associated with superantigenexotoxin. Journal of Clinical Microbiology, 42, 2866-2869

[10]Kuusi M, Lahti E, Virolainen A, Hatakka M, Vuento R,Rantala L, Vuopio-Varkila J, Seuna E, Karppelin M,Hakkinen M, et al. 2006. An outbreak of Streptococcusequi subspecies zooepidemicus associated withconsumption of fresh goat cheese. BMC InfectiousDiseases, 6, 36.Las Heras A, Vela A I, Fernandez E, Legaz E, Dominguez L,Fernandez-Garayzabal J F. 2002. Unusual outbreak ofclinical mastitis in dairy sheep caused by Streptococcusequi subsp. zooepidemicus. Journal of ClinicalMicrobiology, 40, 1106-1108

[11]Lindmark H, Jacobsson K, Frykberg L, Guss B. 1996.Fibronectin-binding protein of Streptococcus equisubsp. zooepidemicus. Infection and Immunity, 64,3993-3999

[12]Linton K J, Higgins C F. 1998. The Escherichia coli ATPbindingcassette (ABC) proteins. MolecularMicrobiology, 28, 5-13

[13]Liu P H, Shun S F, Wang Y K, Zhang S H. 2001.Identification of swine streptococcus isolates inShanghai. Chinese Journal of Veterinary Medicine,21, 42-46

[14]Lun S, Willson P J. 2005. Putative mannose-specificphosphotransferase system component IID repressesexpression of suilysin in serotype 2 Streptococcus suis.Veterinary Microbiology, 105, 169-180

[15]Parsons Y N, Panagea S, Smart C H, Walshaw M J, Hart CA, Winstanley C. 2002. Use of subtractive hybridizationto identify a diagnostic probe for a cystic fibrosisepidemic strain of Pseudomonas aeruginosa. Journalof Clinical Microbiology, 40, 4607-4611

[16]Radnedge L, Gamez-Chin S, McCready P M, Worsham P L,Andersen G L. 2001. Identification of nucleotidesequences for the specific and rapid detection ofYersinia pestis. Applied and EnvironmentalMicrobiology, 67, 3759-3762

[17]Raphel C F, Beech J. 1982. Pleuritis secondary to pneumoniaor lung abscessation in 90 horses. Journal of theAmerican Veterinary Medical Association, 181, 808-810

[18]Rosinha G M, Freitas D A, Miyoshi A, Azevedo V, CamposE, Cravero S L, Rossetti O, Splitter G, Oliveira S C. 2002.Identification and characterization of a Brucella abortusATP-binding cassette transporter homolog toRhizobium meliloti ExsA and its role in virulence andprotection in mice. Infection and Immunity, 70, 5036-5044

[19]Sambrook J, Fritsch E F, Maniatis T. 1989. MolecularCloning: A Laboratory Manual. Cold Spring HarborLaboratory, Cold Spring Harbor, NY.Sawada K, Kokeguchi S, Hongyo H, Sawada S, MiyamotoM, Maeda H, Nishimura F, Takashiba S, Murayama Y.1999. Identification by subtractive hybridization of anovel insertion sequence specific for virulent strainsof Porphyromonas gingivalis. Infection and Immunity,67, 5621-5625

[20]Sharp M W, Prince M J, Gibbens J. 1995. S. zooepidemicusinfection and bovine mastitis. Veterinary Record, 137,128.

[21]Soedarmanto I, Pasaribu F H, Wibawan I W, Lammler C.1996. Identification and molecular characterization ofserological group C streptococci isolated from diseasedpigs and monkeys in Indonesia. Journal of ClinicalMicrobiology, 34, 2201-2204

[22]Starr C R, Engleberg N C. 2006. Role of hyaluronidase insubcutaneous spread and growth of group Astreptococcus. Infection and Immunity, 74, 40-48

[23]Strauss E J, Falkow S. 1997. Microbial pathogenesis:genomics and beyond. Science, 276, 707-712

[24]Timoney J F, Walker J, Zhou M, Ding J. 1995. Cloning andsequence analysis of a protective M-like protein genefrom Streptococcus equi subsp. zooepidemicus.Infection and Immunity, 63, 1440-1445

[25]Ural O, Tuncer I, Dikici N, Aridogan B. 2003. Streptococcuszooepidemicus meningitis and bacteraemia.Scandinavian Journal of Infectious Diseases, 35, 206-207

[26]Younan M, Estoepangestie A T, Cengiz M, Alber J, El-Sayed A, Lammler C. 2005. Identification and molecularcharacterization of Streptococcus equi subsp.zooepidemicus isolated from camels (Camelusdromedarius) and camel milk in Kenya and Somalia.Journal of Veterinary Medicine (Series B - InfectiousDiseases and Veterinary Public Health), 52, 142-146

[27]Young J, Holland I B. 1999. ABC transporters: bacterialexporters-revisited five years on. Biochimica etBiophysica Acta, 1461, 177-200.

Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus

Identification and Characterization of Putative Virulent Genes in Streptococcus equi ssp. zooepidemicus

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