Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze

doi:10.1016/S2095-3119(13)60360-3

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (4): 804-810.DOI: 10.1016/S2095-3119(13)60360-3

Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze

HUO Jing-qian, XING Ji-hong, ZHANG Li-hui, KANG Zhan-hai , ZHANG Jin-lin

1、College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China
2、College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China

收稿日期:2013-01-22 出版日期:2014-04-01 发布日期:2014-04-16
通讯作者: ZHANG Jin-lin, Tel: +86-312-7528575, E-mail: zhangjinlin@hebau.edu.cn
作者简介:HUO Jing-qian, E-mail: huojingqian@163.com
基金资助:
This research was supported by the National Natural Science Foundation of China (31171877).

Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze

HUO Jing-qian, XING Ji-hong, ZHANG Li-hui, KANG Zhan-hai , ZHANG Jin-lin

1、College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China
2、College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China

Received:2013-01-22 Online:2014-04-01 Published:2014-04-16
Contact: ZHANG Jin-lin, Tel: +86-312-7528575, E-mail: zhangjinlin@hebau.edu.cn
About author:HUO Jing-qian, E-mail: huojingqian@163.com
Supported by:
This research was supported by the National Natural Science Foundation of China (31171877).

摘要/Abstract

摘要： In order to understand the composition and structure of herbicidal active substance from the root of Flaveria bidentis (L.) Kuntze, the isolation and structural identification were researched in this paper. The crude extract from the root of F. bidentis (L.) Kuntze was extracted by petroleum ether, ethyl acetate, and water saturation of n-butyl alcohol, respectively, and the extraction fluid was separated by using the method of TLC, then the main fraction was separated by HPLC, and the structure of the herbicidal active substance was analyzed by LC-MS, elemental analysis and 1H-NMR. The results showed that the petroleum extraction had the strongest herbicidal activity, and the purple blue stripe separated by TLC had the strongest effect on Digitaria sanguinalis. The herbicidal active substance was identified as α-terthienyl according to the data of LC-MS, elemental analysis and 1H-NMR.

关键词: Flaveria bidentis (L.) Kuntze , herbicidal active substance , isolation , structure identification

Abstract: In order to understand the composition and structure of herbicidal active substance from the root of Flaveria bidentis (L.) Kuntze, the isolation and structural identification were researched in this paper. The crude extract from the root of F. bidentis (L.) Kuntze was extracted by petroleum ether, ethyl acetate, and water saturation of n-butyl alcohol, respectively, and the extraction fluid was separated by using the method of TLC, then the main fraction was separated by HPLC, and the structure of the herbicidal active substance was analyzed by LC-MS, elemental analysis and 1H-NMR. The results showed that the petroleum extraction had the strongest herbicidal activity, and the purple blue stripe separated by TLC had the strongest effect on Digitaria sanguinalis. The herbicidal active substance was identified as α-terthienyl according to the data of LC-MS, elemental analysis and 1H-NMR.

Key words: Flaveria bidentis (L.) Kuntze , herbicidal active substance , isolation , structure identification

HUO Jing-qian, XING Ji-hong, ZHANG Li-hui, KANG Zhan-hai , ZHANG Jin-lin. Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze[J]. Journal of Integrative Agriculture, 2014, 13(4): 804-810.

参考文献

Ananvoranich S, Varin L, Gulick P, Ibrahim R. 1994. Cloning and regulation of flavonol 3-sulfotransferase in cell-suspension cultures of Flaveria bidentis. Plant Physiology, 106, 485-491

Batish D R, Arora K, Singh H P, Kohli R K. 2007. Potential utilization of dried powder of Tagetes minuta as a natural herbicide for managing rice weeds. Crop Protection, 26, 566-571

Chai Q, Huang G B, Huang P, Zhang E H, Feng F X. 2005. Identification of cicer arietinum root exudation and allelopathy of benzaldhyde. Acta Prataculturae Sinica, 14, 106-111 (in Chinese)

David K W, Larhy J Z, Cahl D W, James E B, Wolfgang B, James E T. 1997. Toxicity of fractionated and degraded Mexican Marigold floral extract to adult Sitophilus zeamais (Coleoptera: Curculionidae). Journal of Econo Micentomology, 90, 1678-1683

Dayan F E, Cantrell C L, Duke S O. 2009. Natural products in crop protection. Bioorganic & Medicinal Chemistry, 17, 4022-4034

Duke S O, Cantrell C L, Meepagala K M, Wedge D E, Tabanca N, Schrader K K. 2010. Natural toxins for use in pest management. Toxins, 2, 1943-1962

Gao X M, Tang T G, Liang Y, Zheng T X, Sang W G, Chen Y L. 2004. An alert regarding biological invasion by a new exotic plant, Flaveria bidentis, and strategies for its control. Chinese Biodiversity, 12, 274-279

Gidda S K, Varin L. 2006. Biochemical and molecular characterization of flavonoid 7-sulfotransferase from Arabidopsis thaliana. Plant Physiology and Biochemistry, 44, 628-636

Guglielmone H A, Agnese A M, Núñez Montoya S C, Cabrera J L. 2005. Inhibitory effects of sulphated flavonoids isolated from Flaveria bidentis on platelet aggregation. Thrombosis Research, 115, 495-502

Liao M, Xie X M, Peng Y, Ma A L. 2011. Changes of soil microbiological characteristics after Solidago canadensis L. invasion. Agricultural Sciences in China, 10, 1064- 1071.

Mares D, Tosi B, Poli F, Andreotti E, Romagnoli C. 2004. Antifungal activity of Tagetes patula extracts on some phytopathogenic fungi: ultrastructural evidence on Pythium ultimum. Microbiological Research, 159, 295- 304.

Marotti I, Marotti M, Piccaglia R, Nastri A, Grandi S, Dinelli G. 2010. Thiophene occurrence in different Tagetes species: agricultural biomasses as sources of biocidal substances. Journal of the Science of Food and Agriculture, 90, 1210-1217

Paterson I, Anderson E A. 2005. The renaissance of natural products as drug candidates. Science, 310, 451-453

Ren Y P, Jiang S, Gu S, Wang Y Z, Zheng S X. 2008. Advances in Flaveria bidentis (L.) Kuntze, a new exotic plant. Journal of Tropical and Subtropical Botany, 16, 390-396

Stuebler H, Kraehmer H, Hess M. 2008. Global changes in crop production and impact trends in weed management - an industry view. In: Proceedings of the 5th International Weed Science Congress. University of Georgia Press, Vancouver, Canada. pp. 309-319

Wang X G, Wei X Y, Huang X Y, Shen L T, Tian Y Q, Xu H H. 2011. Insecticidal constructure and bioactivities of compounds from Ficus sarmentosa var. henryi. Agricultural Sciences in China, 10, 1402-1409

Xie Q Q, Wei Y, Zhang G L. 2010. Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high- speed counter-current chromatography. Separation and Purification Technology, 72, 229-233

Xu J, Xu W C, Yang Y J, Tao B, Zhang J L. 2008. The allelopathy of Flaveria bidentis (L.) Kuntze, an invasive weed species. Frontiers of Agriculture in China, 2, 446- 450.

Zhang F J, Guo J Y, Chen F X, Guo A Y, Wan F H. 2012. Assessment of allelopathic effects of residues of Flaveria bidentis (L.) Kuntze on wheat seedlings. Archives of Agronomy and Soil Science, 58, 257-265

Zhang J L, Zhang L H, Liu Y C, Ma J, Li C, Dong J G. 2006. The herbicidal activity of mutant isolates from Botrytis cinerea. Agricultural Sciences in China, 5, 622- 628.

[1]	YANG Xing, ZHANG Li-hui, SHI Cui-ping, SHANG Yan, ZHANG Jin-lin, HAN Jian-min , DONG. The Extraction, Isolation and Identification of Exudates from the Roots of Flaveria bidentis[J]. Journal of Integrative Agriculture, 2014, 13(1): 105-114.
[2]	LI Jing, LIU Xin-gang, DONG Feng-shou, XU Jun, GUO Li-qun, KONG Zhi-qiang, TIAN Ying-ying, WU Yan-bin , ZHENG Yong-quan. ASimpleMethod for the Isolation andPurification of 2,4-Dihydroxy-7-Methoxy- 2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings[J]. Journal of Integrative Agriculture, 2013, 12(1): 95-102.
[3]	ZHAO Guang-wei, SHEN Bo, XIE Qing, XU Li-xin, YAN Ruo-feng, SONG Xiao-kai, Hassan Ibrahim Adam, LI Xiang-rui. Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China[J]. Journal of Integrative Agriculture, 2012, 12(8): 1347-1353.
[4]	HU Zhi-yong, ZHAN Gao-miao, WANG Han-zhong, HUA Wei. A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue[J]. Journal of Integrative Agriculture, 2012, 12(7): 1212-1215.
[5]	LIU Shu-sheng, John Colvin , Paul J De Barro. Species Concepts as Applied to the Whitefly Bemisia tabaci Systematics: How Many Species Are There?[J]. Journal of Integrative Agriculture, 2012, 11(2): 176-186.
[6]	LI Xiao-xi, LI Shao-jian, XUE Xia, Muhammad Z Ahmed, REN Shun-xiang, rew G S Cuthbertson , QIU Bao-li. The Effects of Space Dimension and Temperature on the Cross Mating of Three Cryptic Species of the Bemisia tabaci Complex in China[J]. Journal of Integrative Agriculture, 2012, 11(2): 197-205.

Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze

Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics