Identification of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) Lam.)

doi:10.1016/S2095-3119(13)60357-3

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (2): 310-315.DOI: 10.1016/S2095-3119(13)60357-3

Identification of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) Lam.)

YU Xiao-xia, ZHAO Ning, LI Hui, JIE Qin, ZHAI Hong, HE Shao-zhen, LI Qiang , LIU Qing-chang

1.Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement/China Agricultural University, Beijing 100193, P.R.China
2.Xuzhou Sweetpotato Research Center, Xuzhou 221121, P.R.China

收稿日期:2012-12-01 出版日期:2014-02-01 发布日期:2014-02-06
通讯作者: LIU Qing-chang, Tel/Fax: +86-10-62733710, E-mail: liuqc@cau.edu.cn
作者简介:YU Xiao-xia
基金资助:
This work was supported by the China Agriculture Research System (CARS-11, Sweetpotato) and the National High- Tech R&D Program of China (2012AA101204).

Identification of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) Lam.)

YU Xiao-xia, ZHAO Ning, LI Hui, JIE Qin, ZHAI Hong, HE Shao-zhen, LI Qiang , LIU Qing-chang

1.Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement/China Agricultural University, Beijing 100193, P.R.China
2.Xuzhou Sweetpotato Research Center, Xuzhou 221121, P.R.China

Received:2012-12-01 Online:2014-02-01 Published:2014-02-06
Contact: LIU Qing-chang, Tel/Fax: +86-10-62733710, E-mail: liuqc@cau.edu.cn
About author:YU Xiao-xia
Supported by:
This work was supported by the China Agriculture Research System (CARS-11, Sweetpotato) and the National High- Tech R&D Program of China (2012AA101204).

摘要/Abstract

摘要： Sweetpotato (Ipomoea batatas (L.) Lam.) breeding is challenging due to its genetic complexity. In the present study, interval mapping (IM) and multiple quantitative trait locus (QTL) model (MQM) analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them, DMFN_4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the first report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.

关键词: co-localized marker , Ipomoea batatas (L.) Lam. , QTLs , starch content

Abstract: Sweetpotato (Ipomoea batatas (L.) Lam.) breeding is challenging due to its genetic complexity. In the present study, interval mapping (IM) and multiple quantitative trait locus (QTL) model (MQM) analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them, DMFN_4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the first report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.

Key words: co-localized marker , Ipomoea batatas (L.) Lam. , QTLs , starch content

YU Xiao-xia, ZHAO Ning, LI Hui, JIE Qin, ZHAI Hong, HE Shao-zhen, LI Qiang , LIU Qing-chang. Identification of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) Lam.)[J]. Journal of Integrative Agriculture, 2014, 13(2): 310-315.

参考文献

Cervantes-Flores J C, Sosinski B, Pecota K V, Mwanga R O M, Catignani G L, Truong V D, Watkins R H, Ulmer M R, Yencho G C. 2011. Identification of quantitative trait loci for dry-matter, starch, and β-carotene content in sweetpotato. Molecular Breeding, 28, 201-216

Cervantes-Flores J C, Yencho G C, Kriegner A, Pecota K V, Faulk M A, Mwanga R O M, Sosinski B R. 2008. Development of a genetic linkage map and identification of homologous linkage groups in sweetpotato using multiple-dose AFLP markers. Molecular Breeding, 21, 511-532

CIP. 2010. International Potato Center About Sweetpotato. [2012-10-28] http://www.cipotato.org/sweetpotato/ Li A X, Liu Q C, Wang Q M, Zhai H, Yan W Z, Zhang H Y, Li M. 2010. Mapping QTLs for starch content in sweetpotato. Molecular Plant Breeding, 8, 516-520 (in Chinese)

van Ooijen J W. 2004. MapQTL 4.0, Software for the Mapping of Quantitative Trait Loci in Experimental Populations. Plant Research International, Kyazma, Wageningen, Netherlands.

Tanksley S D. 1993. Mapping polygenes. Annual Review of Genetics, 27, 205-233

Voorrips R E. 2002. MapChart: Software for the graphical presentation of linkage maps and QTLs. Journal of Heredity, 93, 77-78

Wang W Z, Yi F, Du S R, Wei X L, Xu L P, Cao H L. 1989. Conversion table of the starch content in sweetpotato. Acta Agronomica Sinica, 15, 94-96 (in Chinese)

Wu J, Tan W F, He J R, Pu Z G, Wang D Y, Zhang Z S, Zhang F F, Yan W Z. 2005. Construction of SRAP linkage map and QTL mapping for starch content in sweetpotato. Molecular Plant Breeding, 3, 841-845. (in Chinese)

Yamakawa O. 1998. Developent of sweetpotato cultivars for new processing use in Japan. Tropical Agriculture, 75, 284-287

Young N D. 1996. QTL mapping and quantitative disease resistance in plants. Annual Review of Phytopathology, 34, 479-501

Zang N, Zhai H, Gao S, Chen W, He S Z, Liu Q C. 2009. Efficient production of transgenic plants using the bar gene for herbicide resistance in sweetpotato. Scientia Horticulturae, 122, 649-653 (in Chinese)

Zhao N. 2012. Construction of high-density molecular linkage maps of sweetpotato, Ipomoea batatas (L.) Lam. PhD thesis, China Agricultural University, China. (in Chinese)

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Identification of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) Lam.)

Identification of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) Lam.)

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