Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (11): 2103-2113.DOI: 10.1016/S2095-3119(13)60396-2

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Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

 ZHANG Chao, LIU Guo-bin, XUE Sha , XIAO Lie   

  1. 1.State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, P.R.China
    2.Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, P.R.China
  • 收稿日期:2012-10-23 出版日期:2013-11-01 发布日期:2013-12-08
  • 通讯作者: Correspondence XUE Sha, Mobile: 13679211517, E-mail: xuesha100@163.com
  • 作者简介:ZHANG Chao, Mobile: 13669200244, E-mail: zhangchaolynn@163.com
  • 基金资助:

    This work was supported by the Strategic Technology Project of Chinese Academy of Sciences (XDA05060300), and the Science and Technology Research and Development Program of Shaanxi Province, China (2011KJXX63).

Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

 ZHANG Chao, LIU Guo-bin, XUE Sha , XIAO Lie   

  1. 1.State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, P.R.China
    2.Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, P.R.China
  • Received:2012-10-23 Online:2013-11-01 Published:2013-12-08
  • Contact: Correspondence XUE Sha, Mobile: 13679211517, E-mail: xuesha100@163.com
  • About author:ZHANG Chao, Mobile: 13669200244, E-mail: zhangchaolynn@163.com
  • Supported by:

    This work was supported by the Strategic Technology Project of Chinese Academy of Sciences (XDA05060300), and the Science and Technology Research and Development Program of Shaanxi Province, China (2011KJXX63).

摘要: The Loess Plateau in China is one of the most eroded areas in the world. Accordingly, vegetation restoration has been implemented in this area over the past two decades to remedy the soil degradation problem. Understanding the microbial community structure is essential for the sustainability of ecosystems and for the reclamation of degraded arable land. This study aimed to determine the effect of different vegetation types on microbial processes and community structure in rhizosphere soils in the Loess Plateau. The six vegetation types were as follows: two natural grassland (Artemisia capillaries and Heteropappus altaicus), two artificial grassland (Astragalus adsurgens and Panicum virgatum), and two artificial shrubland (Caragana korshinskii and Hippophae rhamnoides) species. The microbial community structure and functional diversity were examined by analyzing the phospholipid fatty acids (PLFAs) and community-level physiological profiles. The results showed that rhizosphere soil sampled from the H. altaicus and A. capillaries plots had the highest values of microbial biomass C, average well color development of carbon resources, Gram-negative (G-) bacterial PLFA, bacterial PLFA, total PLFA, Shannon richness, and Shannon evenness, as well as the lowest metabolic quotient. Soil sampled from the H. rhamnoides plots had the highest metabolic quotient and Gram-positive (G+) bacterial PLFA, and soil sampled from the A. adsurgens and A. capillaries plots had the highest fungal PLFA and fungal:bacterial PLFA ratio. Correlation analysis indicated a significant positive relationship among the microbial biomass C, G- bacterial PLFA, bacterial PLFA, and total PLFA. In conclusion, plant species under arid climatic conditions significantly affected the microbial community structure in rhizosphere soil. Among the studied plants, natural grassland species generated the most favorable microbial conditions.

关键词: soil microbial biomass , microbial community structure , PLFA , community-level physiological profiles , vegetation types

Abstract: The Loess Plateau in China is one of the most eroded areas in the world. Accordingly, vegetation restoration has been implemented in this area over the past two decades to remedy the soil degradation problem. Understanding the microbial community structure is essential for the sustainability of ecosystems and for the reclamation of degraded arable land. This study aimed to determine the effect of different vegetation types on microbial processes and community structure in rhizosphere soils in the Loess Plateau. The six vegetation types were as follows: two natural grassland (Artemisia capillaries and Heteropappus altaicus), two artificial grassland (Astragalus adsurgens and Panicum virgatum), and two artificial shrubland (Caragana korshinskii and Hippophae rhamnoides) species. The microbial community structure and functional diversity were examined by analyzing the phospholipid fatty acids (PLFAs) and community-level physiological profiles. The results showed that rhizosphere soil sampled from the H. altaicus and A. capillaries plots had the highest values of microbial biomass C, average well color development of carbon resources, Gram-negative (G-) bacterial PLFA, bacterial PLFA, total PLFA, Shannon richness, and Shannon evenness, as well as the lowest metabolic quotient. Soil sampled from the H. rhamnoides plots had the highest metabolic quotient and Gram-positive (G+) bacterial PLFA, and soil sampled from the A. adsurgens and A. capillaries plots had the highest fungal PLFA and fungal:bacterial PLFA ratio. Correlation analysis indicated a significant positive relationship among the microbial biomass C, G- bacterial PLFA, bacterial PLFA, and total PLFA. In conclusion, plant species under arid climatic conditions significantly affected the microbial community structure in rhizosphere soil. Among the studied plants, natural grassland species generated the most favorable microbial conditions.

Key words: soil microbial biomass , microbial community structure , PLFA , community-level physiological profiles , vegetation types