Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis

doi:10.1016/S2095-3119(13)60238-5

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (3): 387-397.DOI: 10.1016/S2095-3119(13)60238-5

• 论文 • 下一篇

Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis

JIANG Qi-yan, HU Zheng, PAN Xing-lai , ZHANG Hui

1.National Key Facilities for Crop Genetic Resources and Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Food Crop Science Department, Cotton Research Institute, Shanxi Academy of Agriculture Sciences, Yuncheng 044000, P.R.China

收稿日期:2012-04-13 出版日期:2013-03-01 发布日期:2013-03-10
通讯作者: Correspondence ZHANG Hui, Tel: +86-10-82108746, Fax: +86-10-62816649, E-mail: zhanghui06@caas.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (30440047) and the Program for Germplasm Innovation and Utilization from the Ministry of Agriculture of China (NB2010-2130135).

Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis

JIANG Qi-yan, HU Zheng, PAN Xing-lai , ZHANG Hui

1.National Key Facilities for Crop Genetic Resources and Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Food Crop Science Department, Cotton Research Institute, Shanxi Academy of Agriculture Sciences, Yuncheng 044000, P.R.China

Received:2012-04-13 Online:2013-03-01 Published:2013-03-10
Contact: Correspondence ZHANG Hui, Tel: +86-10-82108746, Fax: +86-10-62816649, E-mail: zhanghui06@caas.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (30440047) and the Program for Germplasm Innovation and Utilization from the Ministry of Agriculture of China (NB2010-2130135).

摘要/Abstract

摘要： Hybrid necrosis is the gradual premature death of leaves or plants in certain F1 hybrids of wheat (Triticum aestivum L.). Comparison of protein expression in necrotic and normal wheat leaves showed that the abundance of 33 proteins was changed significantly, and 24 of these proteins were identified. These proteins were involved in plant growth and development, antioxidation, photosynthesis and carbon assimilation, amino acid and protein biosynthesis, cytological signal transduction, DNA and RNA modification, protein transport, folding and assembly according to their functions. The down-regulation of uroporphyrinogen decarboxylase and the up-regulation of lipoxygenases in necrotic leaves may be related to the oxidative stress in the necrotic cells. The heat shock proteins may play the cytoprotective role. The differential expression of photosynthesis and carbon assimilation related proteins indicated chlorophyll biosynthesis and chloroplast development were inhibited and might finally cause the gradual chlorosis and cell death in necrotic leaves. The results of this study give a comprehensive picture of the post-transcriptional response to necrosis in hybrid wheat leaves and serve as a platform for further characterization of gene function and regulation in wheat hybrid necrosis.

关键词: hybrid necrosis , proteomic , wheat , differentially expressed protein

Abstract: Hybrid necrosis is the gradual premature death of leaves or plants in certain F1 hybrids of wheat (Triticum aestivum L.). Comparison of protein expression in necrotic and normal wheat leaves showed that the abundance of 33 proteins was changed significantly, and 24 of these proteins were identified. These proteins were involved in plant growth and development, antioxidation, photosynthesis and carbon assimilation, amino acid and protein biosynthesis, cytological signal transduction, DNA and RNA modification, protein transport, folding and assembly according to their functions. The down-regulation of uroporphyrinogen decarboxylase and the up-regulation of lipoxygenases in necrotic leaves may be related to the oxidative stress in the necrotic cells. The heat shock proteins may play the cytoprotective role. The differential expression of photosynthesis and carbon assimilation related proteins indicated chlorophyll biosynthesis and chloroplast development were inhibited and might finally cause the gradual chlorosis and cell death in necrotic leaves. The results of this study give a comprehensive picture of the post-transcriptional response to necrosis in hybrid wheat leaves and serve as a platform for further characterization of gene function and regulation in wheat hybrid necrosis.

Key words: hybrid necrosis , proteomic , wheat , differentially expressed protein

JIANG Qi-yan, HU Zheng, PAN Xing-lai, ZHANG Hui. Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis[J]. Journal of Integrative Agriculture, 2013, 12(3): 387-397.

JIANG Qi-yan, HU Zheng, PAN Xing-lai , ZHANG Hui. Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis[J]. Journal of Integrative Agriculture, 2013, 12(3): 387-397.

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Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis

Comparative Proteomic Analysis of Wheat (Triticum aestivum L.) Hybrid Necrosis

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