Microbial Community in the Forestomachs of Alpacas (Lama pacos) and Sheep (Ovis aries)

doi:10.1016/S2095-3119(13)60230-0

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (2): 314-318.DOI: 10.1016/S2095-3119(13)60230-0

Microbial Community in the Forestomachs of Alpacas (Lama pacos) and Sheep (Ovis aries)

PEI Cai-xia, LIU Qiang, DONG Chang-sheng, LI Hong-quan, JIANG Jun-bing , GAO Wen-jun

College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.China

收稿日期:2012-07-20 出版日期:2013-02-01 发布日期:2013-02-06
通讯作者: Correspondence DONG Chang-sheng, Tel: +86-354-6288221, Fax: +86-354-6222942, E-mail:cs_dong@sxau.edu.cn
作者简介:PEI Cai-xia, Mobile: 13753449871, E-mail: peicaixia@yahoo.com.cn
基金资助:
This work was supported by the Postdoctoral Foundation of Shanxi Agricultural University and the Key Scientific and Technological Project of Shanxi Province, China (20110311031).

Microbial Community in the Forestomachs of Alpacas (Lama pacos) and Sheep (Ovis aries)

PEI Cai-xia, LIU Qiang, DONG Chang-sheng, LI Hong-quan, JIANG Jun-bing , GAO Wen-jun

College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.China

Received:2012-07-20 Online:2013-02-01 Published:2013-02-06
Contact: Correspondence DONG Chang-sheng, Tel: +86-354-6288221, Fax: +86-354-6222942, E-mail:cs_dong@sxau.edu.cn
About author:PEI Cai-xia, Mobile: 13753449871, E-mail: peicaixia@yahoo.com.cn
Supported by:
This work was supported by the Postdoctoral Foundation of Shanxi Agricultural University and the Key Scientific and Technological Project of Shanxi Province, China (20110311031).

摘要/Abstract

摘要： Four 2-yr old alpacas ((48±2.3) kg) and four 2-yr old sheep ((50±1.7) kg) were used to study the pH and microbial community of forestomach from alpacas (Lama pacos) and sheep (Ovis aries) fed fresh alfalfa as the sole forage at low altitude (793 m). The forestomach fluid was taken anaerobically via the esophagus. The electric pH meter and quantitative polymerase chain reaction systems were used to study the the pH and microbial community of forestomach. The results showed that the mean pH of forestomach fluid from alpacas was higher than that from sheep (P<0.01). The percentages of methanogens and Ruminococcus flavefaciens to total bacterial were lower in the forestomach of alpacas than that in the rumen of sheep, while the percentage of fungi and Fibrobacter succinogenes were higher. The percentage of protozoa was similar in the forestomach of alpacas and sheep. These differences can partly explain the reason that alpacas were lower methane production than sheep.

关键词: forestomach microbial community , alpacas , sheep , alfalfa

Abstract: Four 2-yr old alpacas ((48±2.3) kg) and four 2-yr old sheep ((50±1.7) kg) were used to study the pH and microbial community of forestomach from alpacas (Lama pacos) and sheep (Ovis aries) fed fresh alfalfa as the sole forage at low altitude (793 m). The forestomach fluid was taken anaerobically via the esophagus. The electric pH meter and quantitative polymerase chain reaction systems were used to study the the pH and microbial community of forestomach. The results showed that the mean pH of forestomach fluid from alpacas was higher than that from sheep (P<0.01). The percentages of methanogens and Ruminococcus flavefaciens to total bacterial were lower in the forestomach of alpacas than that in the rumen of sheep, while the percentage of fungi and Fibrobacter succinogenes were higher. The percentage of protozoa was similar in the forestomach of alpacas and sheep. These differences can partly explain the reason that alpacas were lower methane production than sheep.

Key words: forestomach microbial community , alpacas , sheep , alfalfa

PEI Cai-xia, LIU Qiang, DONG Chang-sheng, LI Hong-quan, JIANG Jun-bing , GAO Wen-jun. Microbial Community in the Forestomachs of Alpacas (Lama pacos) and Sheep (Ovis aries)[J]. Journal of Integrative Agriculture, 2013, 12(2): 314-318.

参考文献

[1]Clemens E T, Stevens C E. 1980. A comparison ofgastrointestinal transit time in ten species of mammal.The Journal of Agricultural Science (Cambridge), 94,735-737

[2]Denman S E, McSweeney C S. 2006. Development of a realtimePCR assay for monitoring anaerobic fungal andcellulolytic bacterial populations within the rumen.FEMS Microbiology Ecology, 58, 572-582

[3]Denman S E, Tomkins N, McSweeney C S. 2005. Monitoringthe effect of bromochloromethane on methanogenpopulations within the rumen using qPCR. In: 2ndInternational Symposium on Greenhouse Gases andAnimal Agriculture. ETH Zurich, Switzerland. pp. 112-114

[4]Dulphy J P, Dardillat C, Jailler M, Ballet J M. 1997.Comparative study of forestomach digestion in llamasand sheep. Reproduction Nutrition Development, 37,709-725

[5]Harrison D G, Beever D E, Thompson D H, Osbourn D F.1975. Manipulation of rumen fermentation in sheep byincreasing the rate of flow of water from the rumen. TheJournal of Agricultural Science (Cambridge), 85, 93-101

[6]Heller R V, Gregory P C, Engelhardt W V. 1984. Pattern ofmotility and flow of digesta in the forestomach of thellama (Lama guanicoe F. Glama). Journal ofComparative Physiology (B), 154, 529-533

[7]Hespel R B, Bryant M P. 1979. Efficiency of rumen microbialgrowth influence of some theoretical and experimentalfactors on YATP. Journal of Animal Science, 49, 1640-1659

[8]Hobson P N. 1997. Introduction. In: Hobson P N, Stewart CS, eds., The Rumen Mmicrobial Ecosystem. BlackieAcademic and Professional Publishers, London. pp. 1-9

[9]Isaacson H R, Hinds F C, Bryant M P, Owens F N. 1975.Efficiency of energy utilization by mixed rumen bacteriain continuous culture. Journal of Dairy Science, 58,1645-1659

[10]Jacobson D R, Lindahl I L, McNeill J J, Shaw J C, DoetschR N, Davis R E. 1957. Feedlot bloat studies. II. Physicalfactors involved in the etiology of frothy bloat. Journalof Animal Science, 16, 515-524

[11]Johnson K A, Johnson D E. 1995. Methane emissions fromcattle. Journal of Animal Science, 73, 2483-2492

[12]Jouany J P. 2000. The digestion in camelids a comparisonto ruminants. INRA Productions Animales, 13, 165-176

[13](in French)Lemosquet S, Dardillat C, Jailler M, Dulphy J P. 1996.Voluntary intake and gastric digestion of two hays byllamas and sheep: influence of concentratesupplementation. The Journal of Agricultural Science(Cambridge), 127, 539-548

[14]Liu Q , Dong C S, Li H Q, Yang W Z, Jiang J B, Gao W J, PeiC X, Liang Z Q. 2009. Forestomach fermentationcharacteristics and diet digestibility in alpacas (Lamapacos) and sheep (Ovis aries) fed two forage diets.Animal Feed Science and Technology, 154, 151-159

[15]Maloy G M. 1972. Comparative studies on digestion andfermentation rates in the forestomach of the one-humpedcamel and the zebu steer. Research in VeterinaryScience, 13, 476-481

[16]Pei C X, Liu Q, Dong C S, Li H Q, Jiang J B, Gao W J. 2010.Diversity and abundance of the bacterial 16S rRNA genesequences in forestomach of alpacas (Lama pacos) andsheep (Ovis aries). Anaerobe, 16, 426-432

[17]Russell J B, Wilson D B. 1996. Why are ruminal cellulolyticbacteria unable to digest cellulose at low pH? Journalof Dairy Science, 79, 1503-1509

[18]San Martin F. 1987. Comparative forage selectivity andnutrition of South American camelids and sheep. Ph Dthesis, Texas Tech University, Lubbock, TX. p. 146.San Martin F, Bryant F C. 1989. Nutrition of domestic SouthAmerican llamas and alpacas. Small RuminantResearch, 2, 191-216

[19]Zoetendal E G, Akkermans A D, de Vos W M. 1998.Temperature gradient gel electrophoresis analysis fromhuman fecal samples reveals stable and host-specificcommunities of active bacteria. Applied andEnvironment Microbiology, 64, 3854-3859

Microbial Community in the Forestomachs of Alpacas (Lama pacos) and Sheep (Ovis aries)

Microbial Community in the Forestomachs of Alpacas (Lama pacos) and Sheep (Ovis aries)

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	HAN Ji-long, YANG Min, GUO Ting-ting, LIU Jian-bin, NIU Chun-e, YUAN Chao, YUE Yao-jing, YANG Bo-hui . High gene flows promote close genetic relationship among fine-wool sheep populations (Ovis aries) in China[J]. Journal of Integrative Agriculture, 2016, 15(4): 862-871.
[2]	TU Xiong-bing, FAN Yao-li, JI Ming-shan, LIU Zhong-kuan, XIE Nan, LIU Zhen-yu, ZHANG Ze-hua. Improving a method for evaluating alfalfa cultivar resistance to thrips[J]. Journal of Integrative Agriculture, 2016, 15(3): 600-607.
[3]	KONG Ling-an, WU Du-qing, HUANG Wen-kun, PENG Huan, HE Wen-ting, PENG De-liang. Assaying the potential of twenty-one legume plants in Medicago truncatula and M. sativa for candidate model plants for investigation the interactions with Heterodera glycines[J]. Journal of Integrative Agriculture, 2016, 15(3): 702-704.
[4]	ZHAO Fu-ping, WEI Cai-hong, ZHANG Li, LIU Jia-sen, WANG Guang-kai, ZENG Tao, DU Li-xin. A genome scan of recent positive selection signatures in three sheep populations[J]. Journal of Integrative Agriculture, 2016, 15(1): 162-174.
[5]	XIE Kai-yun, LI Xiang-lin, HE Feng, ZHANG Ying-jun, WAN Li-qiang, David B Hannaway, WANG Dong, QIN Yan, Gamal M A Fadul. Effect of nitrogen fertilization on yield, N content, and nitrogen fixation of alfalfa and smooth bromegrass grown alone or in mixture in greenhouse pots[J]. Journal of Integrative Agriculture, 2015, 14(9): 1864-1876.
[6]	JI Shou-kun, XU Gui-shan, DIao Qi-yu, DenG Kai-dong, JIanG Cheng-gang, TU Yan, ZHanG nai-feng. Macromineral requirements of Dorper×thin-tailed Han crossbred female lambs[J]. Journal of Integrative Agriculture, 2015, 14(8): 1617-1626.
[7]	WANG Li, JIANG Lin-lin, Nomura Mika, Tajima Shigeyuki, CHENG Xian-guo. Excessive ammonia inhibited transcription of MsU2 gene and furthermore affected accumulation distribution of allantoin and amino acids in alfalfa Medicago sativa[J]. Journal of Integrative Agriculture, 2015, 14(7): 1269-1282.
[8]	HAN Ji-long, YANG Min, GUO Ting-ting, YUE Yao-jing, LIU Jian-bin, NIU Chun-e, WANG Chao-feng, YANG Bo-hui. Molecular characterization of two candidate genes associated with coat color in Tibetan sheep (Ovis arise)[J]. Journal of Integrative Agriculture, 2015, 14(7): 1390-1397.
[9]	Pedro A Hernández-García, Alejandro Lara-Bueno, Germán D Mendoza-Martínez, José R Bárcena-Gama, Fernando X Plata-Pérez, Rufino López-Ordaz, José A Martínez-García. Effects of feeding yeast (Saccharomyces cerevisiae), organic selenium and chromium mixed on growth performance and carcass traits of hair lambs[J]. Journal of Integrative Agriculture, 2015, 14(3): 575-582.
[10]	CHEN Liang, GAO Li-xiang, LIU Li, DING Ze-ming, ZHANG Hong-fu. Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs[J]. Journal of Integrative Agriculture, 2015, 14(12): 2598-2604.
[11]	GAO Zhen, PAN Hong-sheng, LIU Bing, LU Yan-hui and LIANG Ge-mei. Performance of Three Adelphocoris spp. (Hemiptera: Miridae) on Flowering and Non-flowering Cotton and Alfalfa[J]. Journal of Integrative Agriculture, 2014, 13(8): 1727-1735.
[12]	CHEN Liang, GAO Li-xiang , ZHANG Hong-fu. Effect of Graded Levels of Fiber from Alfalfa Meal on Nutrient Digestibility and Flow of Fattening Pigs[J]. Journal of Integrative Agriculture, 2014, 13(8): 1746-1752.
[13]	ZHANG Yan, WU Sachula, LUO Fen-hua, Baiyinbatu, LIU Lin-hong, HU Tian-yuan, YU Bo-yang, LI Guang-peng , WU Ying-ji. CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis[J]. Journal of Integrative Agriculture, 2014, 13(8): 1759-1765.
[14]	HU Hong-lian, LIU Yong-zhi, LI Ya-kui, LU De-xun , GAO Min. Use of the N-alkanes to Estimate Intake, Apparent Digestibility and Diet Composition in Sheep Grazing on Stipa breviflora Desert Steppe[J]. Journal of Integrative Agriculture, 2014, 13(5): 1065-1072.
[15]	ZHANG Qian, MA Cui, XUE Xin, XU Ming, LI Jing , WU Jin-xia. Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa[J]. Journal of Integrative Agriculture, 2014, 13(11): 2500-2507.