Angiopoietin-Like Protein 3 Expression is Down-Regulated in Experimentally Pregnant Toxemic Goats

doi:10.1016/S1671-2927(00)8645

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (7): 1181-1188.DOI: 10.1016/S1671-2927(00)8645

Angiopoietin-Like Protein 3 Expression is Down-Regulated in Experimentally Pregnant Toxemic Goats

href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((CHEN Xiao-jun[Author]) AND 1[Journal]) AND year[Order])" target="_blank">CHEN Xiao-jun, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((BAI Xia[Author]) AND 1[Journal]) AND year[Order])" target="_blank">BAI Xia, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((MAI Pei[Author]) AND 1[Journal]) AND year[Order])" target="_blank">MAI Pei, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((CAI Jie[Author]) AND 1[Journal]) AND year[Order])" target="_blank">CAI Jie, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((LIU Zhao-ying[Author]) AND 1[Journal]) AND year[Order])" target="_blank">LIU Zhao-ying, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((WANG Hui[Author]) AND 1[Journal]) AND year[Order])" target="_blank">WANG Hui, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((XIAO Hong-bo[Author]) AND 1[Journal]) AND year[Order])" target="_blank">XIAO Hong-bo, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((DONG Wei[Author]) AND 1[Journal]) AND year[Order])" target="_blank">DONG Wei, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((WANG Shui-lian[Author]) AND 1[Journal]) AND year[Order])" target="_blank">WANG Shui-lian, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=((( SUN Zhi-liang[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> SUN Zhi-liang,

1.College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, P.R.China
2.Engineering Research Center of Veterinary Drugs of Hunan Province, Changsha 410311, P.R.China

收稿日期:2011-12-06 出版日期:2012-07-01 发布日期:2012-07-27
通讯作者: SUN Zhi-liang, Tel/Fax: +86-731-84635229, E-mail: sunzhiliang1965@yahoo.com.cn
作者简介:CHEN Xiao-jun, Mobile: 13787116323, E-mail: xj92e2@163.com;
基金资助:
This work was supported by the National Natural Science Foundation of China (31072167), the Key Project of Hunan Provincial Science & Technology Department, China (2009FJ1005), and Innovation Project of Hunan Provincial Education Department (CX2009B153), China.

Angiopoietin-Like Protein 3 Expression is Down-Regulated in Experimentally Pregnant Toxemic Goats

CHEN Xiao-jun, BAI Xia, MAI Pei, CAI Jie, LIU Zhao-ying, WANG Hui, XIAO Hong-bo, DONG Wei, WANG Shui-lian, SUN Zhi-liang,

1.College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, P.R.China
2.Engineering Research Center of Veterinary Drugs of Hunan Province, Changsha 410311, P.R.China

Received:2011-12-06 Online:2012-07-01 Published:2012-07-27
Contact: SUN Zhi-liang, Tel/Fax: +86-731-84635229, E-mail: sunzhiliang1965@yahoo.com.cn
About author:CHEN Xiao-jun, Mobile: 13787116323, E-mail: xj92e2@163.com;
Supported by:
This work was supported by the National Natural Science Foundation of China (31072167), the Key Project of Hunan Provincial Science & Technology Department, China (2009FJ1005), and Innovation Project of Hunan Provincial Education Department (CX2009B153), China.

摘要/Abstract

摘要： Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 (ANGPTL3) can contribute to disorder of carbohydrate and lipid metabolism. The present study was conducted to investigate the change of ANGPTL3 expression during pregnancy toxemia. We firstly cloned the full-length cDNA of ANGPTL3 in Liuyang Black goats, revealing that goat ANGPTL3 had the typical structure of the angiopoietin-like family, and its mRNA was exclusively expressed in liver. Pregnancy toxemia of pregnant goat does with twins during late gestation was induced by being fasted for 72 h, and then they were recovered after 5 d of refeeding. Hepatic ANGPTL3 gene expression was significantly down-regulated concomitantly with decreased serum glucose concentration, elevated serum β-hydroxybutyrate and free fatty acid levels in pregnant toxemic goats, and these changes were reversed after refeeding. These results suggest ANGPTL3 may play a certain role in the development of pregnancy toxemia in goats.

关键词: angiopoietin-like protein 3, clone, pregnancy toxemia, goat

Abstract: Pregnancy toxemia is a metabolic disorder of lipid and glucose. Recent investigations have found that angiopoietin-like protein 3 (ANGPTL3) can contribute to disorder of carbohydrate and lipid metabolism. The present study was conducted to investigate the change of ANGPTL3 expression during pregnancy toxemia. We firstly cloned the full-length cDNA of ANGPTL3 in Liuyang Black goats, revealing that goat ANGPTL3 had the typical structure of the angiopoietin-like family, and its mRNA was exclusively expressed in liver. Pregnancy toxemia of pregnant goat does with twins during late gestation was induced by being fasted for 72 h, and then they were recovered after 5 d of refeeding. Hepatic ANGPTL3 gene expression was significantly down-regulated concomitantly with decreased serum glucose concentration, elevated serum β-hydroxybutyrate and free fatty acid levels in pregnant toxemic goats, and these changes were reversed after refeeding. These results suggest ANGPTL3 may play a certain role in the development of pregnancy toxemia in goats.

Key words: angiopoietin-like protein 3, clone, pregnancy toxemia, goat

CHEN Xiao-jun, BAI Xia, MAI Pei, CAI Jie, LIU Zhao-ying, WANG Hui, XIAO Hong-bo, DONG Wei, WANG Shui-lian, SUN Zhi-liang, . Angiopoietin-Like Protein 3 Expression is Down-Regulated in Experimentally Pregnant Toxemic Goats[J]. Journal of Integrative Agriculture, 2012, 12(7): 1181-1188.

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Angiopoietin-Like Protein 3 Expression is Down-Regulated in Experimentally Pregnant Toxemic Goats

Angiopoietin-Like Protein 3 Expression is Down-Regulated in Experimentally Pregnant Toxemic Goats

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