Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (8): 1159-1167.DOI: 10.1016/S1671-2927(11)60106-5

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MicroRNAs Involved in the Pathogenesis of Phytophthora Root Rot of Soybean (Glycine max)

  

  1. College of Agriculture, Northeast Agricultural University
  • 收稿日期:2010-06-22 出版日期:2011-08-15 发布日期:2011-08-16
  • 通讯作者: Correspondence CHEN Qing-shan, Professor, E-mail: qshchen@126.com; HU Guo-hua, Professor, E-mail: hugh757@vip.163.com
  • 作者简介:WANG Jing, Ph D, E-mail: buyijingjing@163.com; LIU Chun-yan, Ph D, E-mail: cyliucn@126.com

MicroRNAs Involved in the Pathogenesis of Phytophthora Root Rot of Soybean (Glycine max)

  1. College of Agriculture, Northeast Agricultural University
  • Received:2010-06-22 Online:2011-08-15 Published:2011-08-16
  • Contact: Correspondence CHEN Qing-shan, Professor, E-mail: qshchen@126.com; HU Guo-hua, Professor, E-mail: hugh757@vip.163.com
  • About author:WANG Jing, Ph D, E-mail: buyijingjing@163.com; LIU Chun-yan, Ph D, E-mail: cyliucn@126.com

摘要: Phytophthora root rot is one of the most prevalent diseases in the world, which can infect the seedlings and plants, withsubstantial negative impact on soybean yield and quality. MicroRNAs (miRNAs) are a class of post-transcriptionalregulators of gene expression during growth and development of organisms. A soybean disease-resistance varietySuinong 10 was inoculated with Phytophthora sojae race No. 1, and the specific miRNA resistant expression profile wasacquired by microarray for the first time. Different expressional miRNAs have been found after comparing the results ofthe treated sample with the control sample. Furthermore, the target genes of different expressional miRNAs were predicted.Two miRNAs, cbr-mir-241 and ath-miR854a, regulated the disease-resistance process directly through their targets, someenzymes. Another two miRNAs, gma-miR169a and ath-miR169h, participated in disease-resistance regulation as transcriptionfactors. Similarly, one miRNA, ptc-miR164f, has been reported to regulate the plant development. All of these studieswould be served as the foundation for exploring the resistance mechanism.

关键词: microRNA (miRNA), microarray, soybean (Glycine max), gene expressional regulation

Abstract: Phytophthora root rot is one of the most prevalent diseases in the world, which can infect the seedlings and plants, withsubstantial negative impact on soybean yield and quality. MicroRNAs (miRNAs) are a class of post-transcriptionalregulators of gene expression during growth and development of organisms. A soybean disease-resistance varietySuinong 10 was inoculated with Phytophthora sojae race No. 1, and the specific miRNA resistant expression profile wasacquired by microarray for the first time. Different expressional miRNAs have been found after comparing the results ofthe treated sample with the control sample. Furthermore, the target genes of different expressional miRNAs were predicted.Two miRNAs, cbr-mir-241 and ath-miR854a, regulated the disease-resistance process directly through their targets, someenzymes. Another two miRNAs, gma-miR169a and ath-miR169h, participated in disease-resistance regulation as transcriptionfactors. Similarly, one miRNA, ptc-miR164f, has been reported to regulate the plant development. All of these studieswould be served as the foundation for exploring the resistance mechanism.

Key words: microRNA (miRNA), microarray, soybean (Glycine max), gene expressional regulation