通过靶向敲除GS9基因快速改善水稻外观品质

doi:10.1016/j.jia.2025.04.002

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (10): 4100-4104.DOI: 10.1016/j.jia.2025.04.002

通过靶向敲除GS9基因快速改善水稻外观品质

收稿日期:2025-01-02 修回日期:2025-04-03 接受日期:2025-03-03 出版日期:2025-10-20 发布日期:2025-09-24

Rapid improvement of rice appearance quality by targeted knockout of the GS9 gene

Tao Zhang, Jiaqi Tang, Qianfeng Li, Lin Zhang, Changquan Zhang, Qiaoquan Liu^#, Dongsheng Zhao^#

Key Laboratory of Plant Functional Genomics, Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory, College of Agriculture, Yangzhou University, Yangzhou 225009, China

Received:2025-01-02 Revised:2025-04-03 Accepted:2025-03-03 Online:2025-10-20 Published:2025-09-24
About author:#Correspondence Dongsheng Zhao, E-mail: dszhao@yzu.edu.cn; Qiaoquan Liu, E-mail: qqliu@yzu.edu.cn
Supported by:
This work was supported by grants from the Government of Jiangsu Province, China (BE2023331, JBGS[2021]001, 23KJA210001, BE2021334, BM2022008-02, and KYCX23_3575) and the National Natural Science Foundation of China (31971914).

摘要/Abstract

摘要：

粒形是决定水稻产量和品质的重要因素。功能缺失型gs9等位基因可以产生细长粒形和较低垩白，影响籽粒外观。本研究中我们证实了当前大多数的粳稻品种表现为短圆粒，其主要粒形基因位点上携带相同的等位基因组合。利用CRISPR/Cas9产生的敲除等位基因gs9^KO能够显著改善供试粳稻品种的粒形和垩白，且对产量性状无影响。此外，通过栽培密度试验确认gs9^KO等位基因造成的植株叶夹角略有增加，不影响最终单株产量。结果表明，gs9^KO等位基因在改善籽粒外观品质方面具有广泛的应用潜力。

Tao Zhang, Jiaqi Tang, Qianfeng Li, Lin Zhang, Changquan Zhang, Qiaoquan Liu, Dongsheng Zhao. 通过靶向敲除GS9基因快速改善水稻外观品质[J]. Journal of Integrative Agriculture, 2025, 24(10): 4100-4104.

Tao Zhang, Jiaqi Tang, Qianfeng Li, Lin Zhang, Changquan Zhang, Qiaoquan Liu, Dongsheng Zhao. Rapid improvement of rice appearance quality by targeted knockout of the GS9 gene[J]. Journal of Integrative Agriculture, 2025, 24(10): 4100-4104.

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通过靶向敲除GS9基因快速改善水稻外观品质

Rapid improvement of rice appearance quality by targeted knockout of the GS9 gene

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