Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (10): 4105-4109.DOI: 10.1016/j.jia.2025.03.016

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  • 收稿日期:2024-09-30 修回日期:2025-03-22 接受日期:2025-02-26 出版日期:2025-10-20 发布日期:2025-09-24

Development and application of a double-antigen Sandwich ELISA using capsid protein to detect multispecies antibodies against porcine circovirus type 3

Dexin Li1*, Junhua Deng2*, Yufang Li2, Liying Hao2, Wenqiang Pang3, Zhendong Zhang1, Yi Yang1, Xiangdong Li1, 2#, Kegong Tian2, 3#   

  1. 1Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China

    2 Luoyang Putai Biotechnology Co., Ltd., Luoyang 471003, China

    3 National Research Center for Veterinary Medicine, Luoyang 471003, China

  • Received:2024-09-30 Revised:2025-03-22 Accepted:2025-02-26 Online:2025-10-20 Published:2025-09-24
  • About author:Dexin Li, E-mail: lidexin0123@163.com; Junhua Deng, E-mail: deng.junhua@sslab.com.cn; #Correspondence Xiangdong Li, E-mail: 007352@yzu.edu.cn; Kegong Tian, E-mail: tiankg@263.net * These authors contributed equally to this study.
  • Supported by:

    This study was supported by the National Key Research and Development Program of China (2023YFD1800500), the Jiangsu Innovative and Entrepreneurial Talent Team Project, China (JSSCTD202224), the 111 Project D18007, and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), China. 

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摘要：

猪圆环病毒3型（Porcine circovirus type 3, PCV3）是一种新发病原体，可感染猪、犬、牛、小鼠等多种动物。此前，已有多种间接酶联免疫吸附试验（iELISA）用于检测猪体内PCV3抗体。本研究首次以杆状病毒表达系统（BEVS）制备的PCV3衣壳蛋白（Cap）作为包被抗原，建立了一种双抗原夹心ELISA（DAgS-ELISA），用于检测不同动物血清中的PCV3抗体。利用该方法，对2022年至2024年中国17个省份的猪血清样本进行了大规模血清学调查，结果显示猪群PCV3血清阳性率为55.7%，其中母猪阳性率显著高于其他群体，达86.3%。此外，该方法成功检测到牛和犬血清中的PCV3抗体，阳性率分别为7.7%和4.4%。进一步研究还将其应用于马、昆明小鼠及20种野生动物血清样本的检测，首次在马体内检出PCV3抗体，表明PCV3的宿主范围进一步扩大。本研究结果证实了PCV3具有广泛的宿主谱，同时表明DAgS-ELISA是一种高效、可靠的血清学检测工具，对PCV3的流行病学监测及防控具有重要意义。