First
identification of a novel Aichivirus D in goats with diarrhea

doi:10.1016/j.jia.2023.11.041

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (4): 1442-1446.DOI: 10.1016/j.jia.2023.11.041

• • 上一篇

收稿日期:2023-06-22 接受日期:2023-10-16 出版日期:2024-04-20 发布日期:2024-03-30

First identification of a novel Aichivirus D in goats with diarrhea

Chen Yang^1*, Keha-mo Abi^1*, Hua Yue^{1, 2}, Falong Yang^{1, 2}, Cheng Tang^{1, 2#}

¹College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China
² Key Laboratory of Qinghai–Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu 610041, China

Received:2023-06-22 Accepted:2023-10-16 Online:2024-04-20 Published:2024-03-30
About author:#Correspondence Cheng Tang, E-mail: tangcheng101@163.com * These authors contributed equally to this study.
Supported by:
This work was sponsored by the Southwest Minzu University Double World-Class Project, China (XM2023014), the Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System (SCCXTD-2020-18), the Key Laboratory of Veterinary Medicine of Universities of Sichuan Province of China, and the Fundamental Research Funds for the Central Universities, Southwest Minzu University, China (ZYN2023043).

摘要/Abstract

摘要：

嵴病毒（Kobuvirus, KoV）是微RNA病毒科的一个新的属，包括A型爱知病毒-F型爱知病毒（Aichivirus A-F）6个种，目前只在牛和绵羊中检测到Aichivirus D。我们在采用宏基因组技术鉴定某爆发腹泻的山羊场的腹泻病原时，意外的在粪便中发现了Aichivirus D序列，本实验的目的是研究山羊源Aichivirus D的分子特征并调查其与山羊腹泻的联系。根据病毒宏基因组学技术获得的Aichivirus D部分序列设计引物，成功地从腹泻样本获得了一个近乎全长Aichivirus D基因组（8,461 bp），包含7,524 bp 的完整编码框，与绵羊Aichivirus D的遗传关系最近，可能由绵羊Aichivirus D通过重组进化而来，重组区域位于VP1基因。为进一步调查Aichivirus D与山羊腹泻的联系，从3个爆发腹泻的羊场采集了116份2-60日龄的羔羊粪便样本（61份腹泻样本，55份临床健康粪便样本），采用特异性RT-PCR进行了Aichivirus D的检测。结果表明，腹泻样本中Aichivirus D阳性率为55.74% (34/61)，显著高于健康样本(14.55%，P＜0.001)，且其它常见腹泻病原未检出，提示Aichivirus D与山羊腹泻有关。在此基础上，又从9个阳性样本中扩增出完整的VP0、VP3和VP1基因，与绵羊源Aichivirus D相比，山羊源Aichivirus D的VP0、VP3和VP1基因有独特的氨基酸突变和遗传进化特征。总之，本实验首次证明Aichivirus D感染山羊，且该病毒可能与山羊腹泻有关。该病毒与绵羊Aichivirus D的亲缘关系最为密切，可能是由绵羊AichiVirus D 重组进化而来的。这些结果有助于深入了解Aichivirus D的宿主范围和遗传进化。

. [J]. Journal of Integrative Agriculture, 2024, 23(4): 1442-1446.

Chen Yang, Keha-mo Abi, Hua Yue, Falong Yang, Cheng Tang.

First identification of a novel Aichivirus D in goats with diarrhea [J]. Journal of Integrative Agriculture, 2024, 23(4): 1442-1446.

参考文献

Abi K M, Yu Z, Jing Z Z, Tang C. 2021. Identification of a novel Aichivirus D in sheep. Infection, Genetics and Evolution, 91, 104810.

Abi K M, Yang C, Tang C, Jing Z Z. 2022. Aichivirus C isolate is a diarrhoea‐causing pathogen in goats. Transboundary and Emerging Diseases, 69, e2268–e2275.

Abi K M, Zhang Q, Jing Z Z, Tang C. 2020. First detection and molecular characteristics of caprine kobuvirus in goats in China. Infection, Genetics and Evolution, 85, 104566.

Akagami M, Ito M, Niira K, Kuroda M, Masuda T, Haga K, Tsuchiaka S, Naoi Y, Kishimoto M, Sano K. 2017. Complete genome analysis of porcine kobuviruses from the feces of pigs in Japan. Virus Genes, 53, 593–602.

Barry A F, Ribeiro J, Alfieri A F, Van Der Poel W H, Alfieri A A. 2011. First detection of kobuvirus in farm animals in Brazil and the Netherlands. Infection, Genetics and Evolution, 11, 1811–1814.

Brunker K, Marston D A, Horton D L, Cleaveland S, Fooks A R, Kazwala R, Ngeleja C, Lembo T, Sambo M, Mtema Z J. 2015. Elucidating the phylodynamics of endemic rabies virus in eastern Africa using whole-genome sequencing. Virus Evolution, 1, vev011.

Chen L, Zhu L, Zhou Y C, Xu Z W, Guo W Z, Yang W Y. 2013. Molecular and phylogenetic analysis of the porcine kobuvirus VP1 region using infected pigs from Sichuan Province, China. Virology Journal, 10, 281.

Chen Y S, Chen B C, Lin Y S, Chang J T, Huang T S, Chen J J, Chang T H. 2013. Detection of Aichi virus with antibody targeting of conserved viral protein 1 epitope. Applied Microbiology and Biotechnology, 97, 8529–8536.

Fan S, Sun H, Ying Y, Gao X, Wang Z, Yu Y, Li Y, Wang T, Yu Z, Yang S. 2013. Identification and characterization of porcine kobuvirus variant isolated from suckling piglet in Gansu province, China. Viruses, 5, 2548–2560.

Han X, Zhang W, Xue Y, Shao S. 2011. Sequence analysis reveals mosaic genome of Aichi virus. Virology Journal, 8, 390.

Kang S, Kim Y C. 2018. Genome-based virus taxonomy with the ICTV database extension. Genomics & Informatics, 16, e22.

Kitajima M, Gerba C. 2015. Aichi virus 1: Environmental occurrence and behavior. Pathogens, 4, 256–268.

Melegari I, Di Profio F, Sarchese V, Martella V, Marsilio F, Di Martino B. 2016. First molecular evidence of kobuviruses in goats in Italy. Archives of Virology, 161, 3245–3248.

Oem J K, Lee M H, Lee K K, An D J. 2014. Novel Kobuvirus species identified from black goat with diarrhea. Veterinary Microbiology, 172, 563–567.

Okitsu S, Khamrin P, Thongprachum A, Hidaka S, Kongkaew S, Kongkaew A, Maneekarn N, Mizuguchi M, Hayakawa S, Ushijima H. 2012. Sequence analysis of porcine kobuvirus VP1 region detected in pigs in Japan and Thailand. Virus Genes, 44, 253–257.

Otomaru K, Naoi Y, Haga K, Omatsu T, Uto T, Koizumi M, Masuda T, Yamasato H, Takai H, Aoki H, Tsuchiaka S, Sano K, Okazaki S, Katayama Y, Oba M, Furuya T, Shirai J, Katayama K, Mizutani T, Nagai M. 2016. Detection of novel kobu-like viruses in Japanese black cattle in Japan. Journal of Veterinary Medical Science, 78, 321–324.

Reuter G, Boros Á, Pankovics P, Egyed L. 2010. Kobuvirus in domestic sheep, hungary. Emerging Infectious Diseases, 16, 869–870.

Sobhy N M, Armién A G, Wünschmann A, Muldoon D, Goyal S M, Mor S K. 2020. Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota. Journal of Veterinary Diagnostic Investigation, 32, 873–879.

Wang L, Fredrickson R, Duncan M, Samuelson J, Hsiao S H. 2020. Bovine kobuvirus in calves with diarrhea, United States. Emerging Infectious Diseases, 26, 176.

Yan N, Yue H, Liu Q, Wang G, Tang C, Liao M. 2023. Isolation and characteristics of a novel aichivirus D from yak. Microbiology Spectrum, 11, e0009923.

Yang F, Liu X, Zhou Y, Lyu W, Xu S, Xu Z, Zhu L. 2015. Histopathology of porcine kobuvirus in Chinese piglets. Virologica Sinica, 30, 396–399.

Zhai S L, Zhang H, Lin T, Chen S N, Zhou X, Chen Q L, Lv D H, Wen X H, Zhou X R, Jia C L. 2017. A novel porcine kobuvirus emerged in piglets with severe diarrhoea in China. Transboundary and Emerging Diseases, 64, 1030–1036.

Zhu L, Wang X, Ren J, Kotecha A, Walter T S, Yuan S, Yamashita T, Tuthill T J, Fry E E, Rao Z. 2016. Structure of human Aichi virus and implications for receptor binding. Nature Microbiology, 1, 1–6.

First identification of a novel Aichivirus D in goats with diarrhea

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics