Creation of two hyperactive variants of phytochrome B1 for attenuating shade avoidance syndrome in maize

doi:10.1016/S2095-3119(20)63466-9

摘要/Abstract

摘要：

增加种植密度是提高玉米产量的有效措施。然而，密植容易引发植物的避荫反应综合征，导致抗倒伏能力下降，最终导致产量降低。光敏色素B（phyB）在植物避荫反应中起主导作用。研究表明，将拟南芥PHYB(AtPHYB)104和361位的酪氨酸(Y)改变为苯丙氨酸(F)可以增强其活性。在本研究中，我们通过模拟AtPHYB的Y104F和Y361F的突变形式，创制了两个玉米PHYB1超敏突变体: ZmPHYB1^Y98F和ZmPHYB1^Y359F。在ZmPHYB1自身启动子的驱动下，将ZmPHYB1^Y98F、ZmPHYB1^Y359F和野生型的ZmPHYB1 (ZmPHYB1^WT)转入到拟南芥phyB-9突变体中。在转基因拟南芥中，与ZmPHYB1^WT相比，异源表达ZmPHYB1^Y98F和ZmPHYB1^Y359F增强了对phyB-9相关表型的互补功能。与ZmPHYB1^WT相似，红光处理可诱导ZmPHYB1^Y98F和ZmPHYB1^Y359F蛋白从细胞质中转运到细胞核，并且能够与玉米中的PIF蛋白互作。此外，在模拟遮荫处理下，ZmPHYB1自身启动子驱动的ZmPHYB1^Y98F和ZmPHYB1^Y359F在转基因玉米中的表达可以减弱玉米幼苗的避荫反应综合征，还能够降低玉米植株的株高和穗位高。综上所述，研究结果表明ZmPHYB1^Y98^F和ZmPHYB1^Y359F能够减弱玉米的避荫反应综合征，为培育耐密玉米品种提供了新思路。

关键词: maize , high density planting , maize phytochrome B1 , shade avoidance syndrome

Abstract: Increasing the planting density of maize is an effective measure to improve its yield. However, plants under high planting density tend to trigger shade avoidance syndrome (SAS), reducing lodging resistance and ultimately yield drop. Phytochrome B
(phyB) plays a dominant role in mediating shade avoidance response. This study constructed two hyperactive mutated alleles of maize PHYB1: ZmPHYB1^Y98F (mimicking Y104F of AtPHYB) and ZmPHYB1^Y359F(mimicking Y361F of AtPHYB). Ectopic expression of ZmPHYB1^Y98F and ZmPHYB1^Y359F under the control of the ZmPHYB1 promoter in the Arabidopsis phyB-9 background rendered enhanced activity on complementing the phyB-9 related phenotypes compared with ZmPHYB1^WT. Moreover, similar to the behavior of ZmPHYB1^WT, ZmPHYB1^Y98F and ZmPHYB1^Y359F proteins are localized to the nucleus after red light exposure, and could interact with PIF proteins of maize. In addition, expression of ZmPHYB1^Y98F and ZmPHYB1^Y359F variants under the control of the native ZmPHYB1 promoter attenuated SAS of maize seedlings subjected to simulated shade treatment. It effectively reduced mature maize’s plant height and ear height in field conditions. The results combined demonstrate the utility of ZmPHYB1^Y98F and ZmPHYB1^Y359F for attenuating SAS and breeding high density-tolerant varieties of maize.

. [J]. Journal of Integrative Agriculture, 2022, 21(5): 1253-1265.

ZHAO Yong-ping, ZHAO Bin-bin, WU Guang-xia, MA Xiao-jing, WANG Bao-bao, KONG De-xin, WEI Hong-bin, WANG Hai-yang. Creation of two hyperactive variants of phytochrome B1 for attenuating shade avoidance syndrome in maize[J]. Journal of Integrative Agriculture, 2022, 21(5): 1253-1265.

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