Transgenic japonica rice expressing the cry1C gene is resistant to striped stem borers in Northeast China

doi:10.1016/S2095-3119(20)63279-8

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (11): 2837-2848.DOI: 10.1016/S2095-3119(20)63279-8

所属专题：水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources

• 论文 • 下一篇

收稿日期:2020-03-11 出版日期:2021-11-01 发布日期:2021-09-17

Transgenic japonica rice expressing the cry1C gene is resistant to striped stem borers in Northeast China

JIN Yong-mei, MA Rui, YU Zhi-jing, LIN Xiu-feng

Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Changchun 130033, P.R.China

Received:2020-03-11 Online:2021-11-01 Published:2021-09-17
Contact: Correspondence LIN Xiu-feng, Tel/Fax: +86-431-87063078, E-mail: linxiufeng8581@163.com
About author:JIN Yong-mei, E-mail: ymjin0303@163.com;
Supported by:
This research was supported by grants from the Jilin Provincial Agricultural Science and Technology Innovation Project in China (CXGC2021TD014) and the National Major Project of Breeding for Genetically Modified Organisms in China (2016ZX08001001-001-007).

摘要/Abstract

摘要：

在东北地区，鳞翅目害虫二化螟严重影响水稻产量和品质。本研究利用农杆菌介导法，将Bt抗虫基因cry1C导入到东北粳稻品种吉粳88。利用分子检测和抗Basta发芽试验，从126个转cry1C基因独立转化体中筛选出16个单拷贝纯合转基因株系。通过对cry1C蛋白水平、抗虫性和农艺性状的评价，最终获得4个高抗二化螟且产量高于非转基因对照品种的cry1C转基因株系，JL16、JL23、JL41和JL42。T-DNA侧翼序列分析结果表明，在转基因株系JL42中cry1C基因插入到第11号染色体基因间区，可推测未发生位置效应。本研究培育出4个抗二化螟转基因粳稻品系，为水稻抗虫育种提供了理论基础与新种质资源。

Abstract:

Rice production and quality are seriously affected by the lepidopteran pest, striped stem borer (SSB), in Northeast China. In this study, a synthetic cry1C gene encoding Bacillus thuringiensis (Bt) δ-endotoxin, which is toxic to lepidopteran pest, was transformed into a japonica rice variety (Jigeng 88) in Northeast China by Agrobacterium-mediated transformation. Through molecular detection and the Basta resistance germination assay, a total of 16 single-copy homozygous transgenic lines were obtained from 126 independent transformants expressing cry1C. Finally, four cry1C-transgenic lines (JL16, JL23, JL41, and JL42) were selected by evaluation of the Cry1C protein level, insect-resistance and agronomic traits. The cry1C-transgenic lines had higher resistance to SSB and higher yield compared with non-transgenic (NT) control plants. T-DNA flanking sequence analysis of the transgenic line JL42 showed that the cry1C gene was inserted into the intergenic region of chromosome 11, indicating that its insertion may not interfere with the genes near insertion site. In summary, this study developed four cry1C-transgenic japonica rice lines with high insect resistance and high yield. They can be used as insect-resistant germplasm materials to overcome the problem of rice yield reduction caused by SSB and reduce the use of pesticides in Northeast China.

Key words: striped stem borer , insect resistance , cry1C gene , transgenic japonica rice , T-DNA flanking sequence

. [J]. Journal of Integrative Agriculture, 2021, 20(11): 2837-2848.

JIN Yong-mei, MA Rui, YU Zhi-jing, LIN Xiu-feng. Transgenic japonica rice expressing the cry1C gene is resistant to striped stem borers in Northeast China[J]. Journal of Integrative Agriculture, 2021, 20(11): 2837-2848.

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Transgenic japonica rice expressing the cry1C gene is resistant to striped stem borers in Northeast China

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