Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (3): 528-536.DOI: 10.1016/S2095-3119(15)61203-5

• 论文 • 上一篇    下一篇

Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage

 XU Yu-chao, HOU Xi-lin, XU Wei-wei, SHEN Lu-lu, LÜ Shan-wu, ZHANG Shi-lin, HU Chun-mei   

  1. 1、State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of
    Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2、Agriculture Committee of Feixi County, Hefei 230001, P.R.China
  • 收稿日期:2015-01-12 出版日期:2016-03-07 发布日期:2016-03-09
  • 通讯作者: HU Chun-mei, Tel: +86-25-84395756,E-mail: jjjhcm@njau.edu.cn
  • 作者简介:XU Yu-chao, E-mail: 1049205908@qq.com;
  • 基金资助:

    This work was supported by the Independent Innovation Fund for Agricultural Science and Technology of Jiangsu Province, China (CX(15)1015).

Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage

 XU Yu-chao, HOU Xi-lin, XU Wei-wei, SHEN Lu-lu, LÜ Shan-wu, ZHANG Shi-lin, HU Chun-mei   

  1. 1、State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of
    Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2、Agriculture Committee of Feixi County, Hefei 230001, P.R.China
  • Received:2015-01-12 Online:2016-03-07 Published:2016-03-09
  • Contact: HU Chun-mei, Tel: +86-25-84395756,E-mail: jjjhcm@njau.edu.cn
  • About author:XU Yu-chao, E-mail: 1049205908@qq.com;
  • Supported by:

    This work was supported by the Independent Innovation Fund for Agricultural Science and Technology of Jiangsu Province, China (CX(15)1015).

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摘要： BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What’s more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.

关键词: non-heading Chinese cabbage , stamen-petalody , ethylene-responsive factor , gene expression

Abstract: BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage (Brassica rapa ssp. chinensis) by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What’s more, the expression of BrcERF-B3 was more significant to ABA, MeJA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.

Key words: non-heading Chinese cabbage , stamen-petalody , ethylene-responsive factor , gene expression