Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (2): 295-308.DOI: 10.1016/S2095-3119(15)61051-6

• 论文 • 上一篇    下一篇

Effects of chilling tolerance induced by spermidine pretreatment on antioxidative activity, endogenous hormones and ultrastructure of indica-japonica hybrid rice seedlings

 ZENG Yan-hua, ZAHNG Yu-ping, XIANG Jing, WU Hui, CHEN Hui-zhe, ZHANG Yi-kai, ZHU De-feng   

  1. 1、College of Agronomy, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2、State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China
  • 收稿日期:2015-02-06 出版日期:2016-02-05 发布日期:2016-02-01
  • 通讯作者: ZHU De-feng, Tel/Fax: +86-571-63370373,E-mail: cnrice@qq.com; ZAHNG Yu-ping, Tel/Fax: +86-571-63371376, E-mail: cnrrizyp@163.com
  • 基金资助:

    This work was supported by grants from the the Earmarked Fund for Modern Agro-industry Technology Research System of China (CARS-01-09B), the Natural Science Foundation of Zhejiang Province, China (Y13C130013) and the Special Fund for Basic Scientific Research Business of Central Public Research Tnstitutes, Chinese Academy of Agricultural Sciences (2012RG004-2).

Effects of chilling tolerance induced by spermidine pretreatment on antioxidative activity, endogenous hormones and ultrastructure of indica-japonica hybrid rice seedlings

 ZENG Yan-hua, ZAHNG Yu-ping, XIANG Jing, WU Hui, CHEN Hui-zhe, ZHANG Yi-kai, ZHU De-feng   

  1. 1、College of Agronomy, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2、State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R.China
  • Received:2015-02-06 Online:2016-02-05 Published:2016-02-01
  • Contact: ZHU De-feng, Tel/Fax: +86-571-63370373,E-mail: cnrice@qq.com; ZAHNG Yu-ping, Tel/Fax: +86-571-63371376, E-mail: cnrrizyp@163.com
  • Supported by:

    This work was supported by grants from the the Earmarked Fund for Modern Agro-industry Technology Research System of China (CARS-01-09B), the Natural Science Foundation of Zhejiang Province, China (Y13C130013) and the Special Fund for Basic Scientific Research Business of Central Public Research Tnstitutes, Chinese Academy of Agricultural Sciences (2012RG004-2).

PDF

摘要： Spermidine (Spd) is known to be involved in the regulation of plant responses to chilling stress and counteract the adverse effect of stress conditions. Antioxidant activities, endogenous hormones and ultrastructure change under chilling stress were investigated in indica-japonica hybrid rice seedlings. 12-d-old seedlings were subjected to exogenous Spd (1 mmol L–1) and then a chilling stress (6°C, 4 d) was induced, followed by a subsequent recovery (25°C, 4 d). Results showed that malondialdehyde (MDA) and proline content were enhanced significantly, whereas shoot fresh and dry weights decreased during chilling stress and after recovery; chlorophyll content of chilling-stressed seedlings increased slightly but declined after recovery; additionally, total soluble sugar, sucrose, fructose and starch contents increased significantly during chilling stress, and only soluble sugar and fructose contents were observed in increase after recovery; chilling stress-induced increases in superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities, but declined after recovery, and the level of ascorbate peroxidase was lower during chilling stress and after recovery; however, endogenous indole-3-acetic acid (IAA), zeatin riboside (ZR), gibberellic acid (GA3), and abscisic acid (ABA) levels were induced decreased compared with Spd pretreatment. The microscopic analysis revealed that chilling stress-induced destruction of the chloroplast envelope during chilling stress and increased the number of plastoglobuli along with aberrations in thylakoid membranes after recovery. In contrast, exogenous Spd protected rice seedlings from chilling-induced injuries in terms of lower malondialdehyde, proline and carbohydrates accumulation coupled with increased endogenous hormones metabolism. After recovery, Spd pretreatment chilling-exposed seedlings showed higher activities of antioxidant enzymes and normal physiological function of chloroplasts. These results suggest that Spd could promote effectively chilling tolerance which might be largely attributable to the integrity of cell structure and normal metabolism of endogenous hormones in indica-japonica hybrid rice seedlings.

关键词: polyamines , chilling stress , antioxidative activity , endogenous hormones , ultrastructure , indica-japonica hybrid rice (Oryza sativa L.)

Abstract: Spermidine (Spd) is known to be involved in the regulation of plant responses to chilling stress and counteract the adverse effect of stress conditions. Antioxidant activities, endogenous hormones and ultrastructure change under chilling stress were investigated in indica-japonica hybrid rice seedlings. 12-d-old seedlings were subjected to exogenous Spd (1 mmol L–1) and then a chilling stress (6°C, 4 d) was induced, followed by a subsequent recovery (25°C, 4 d). Results showed that malondialdehyde (MDA) and proline content were enhanced significantly, whereas shoot fresh and dry weights decreased during chilling stress and after recovery; chlorophyll content of chilling-stressed seedlings increased slightly but declined after recovery; additionally, total soluble sugar, sucrose, fructose and starch contents increased significantly during chilling stress, and only soluble sugar and fructose contents were observed in increase after recovery; chilling stress-induced increases in superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities, but declined after recovery, and the level of ascorbate peroxidase was lower during chilling stress and after recovery; however, endogenous indole-3-acetic acid (IAA), zeatin riboside (ZR), gibberellic acid (GA3), and abscisic acid (ABA) levels were induced decreased compared with Spd pretreatment. The microscopic analysis revealed that chilling stress-induced destruction of the chloroplast envelope during chilling stress and increased the number of plastoglobuli along with aberrations in thylakoid membranes after recovery. In contrast, exogenous Spd protected rice seedlings from chilling-induced injuries in terms of lower malondialdehyde, proline and carbohydrates accumulation coupled with increased endogenous hormones metabolism. After recovery, Spd pretreatment chilling-exposed seedlings showed higher activities of antioxidant enzymes and normal physiological function of chloroplasts. These results suggest that Spd could promote effectively chilling tolerance which might be largely attributable to the integrity of cell structure and normal metabolism of endogenous hormones in indica-japonica hybrid rice seedlings.

Key words: polyamines , chilling stress , antioxidative activity , endogenous hormones , ultrastructure , indica-japonica hybrid rice (Oryza sativa L.)