QTL consistency for agronomic traits across three generations and potential applications in popcorn

doi:10.1016/S2095-3119(15)61060-7

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (12): 2547-2557.DOI: 10.1016/S2095-3119(15)61060-7

QTL consistency for agronomic traits across three generations and potential applications in popcorn

DONG Yong-bin, ZHANG Zhong-wei, SHI Qing-ling, WANG Qi-lei, ZHOU Qiang, DENG Fei, MA Zhi-yan, QIAO Da-he, LI Yu-ling

College of Agronomy, Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, P.R.China

收稿日期:2014-12-03 出版日期:2015-12-01 发布日期:2015-12-16
通讯作者: LI Yu-ling, Tel: +86-371-63555540,Fax: +86-371-63558126, E-mail: yuling_li@126.com
作者简介:DONG Yong-bin, Tel: +86-371-63555540, E-mail: dyb0816@163.com;
基金资助:
This work was funded by the Plan for the Scientific Innovation Talent of Henan Province, China (124200510003), the National High-Tech Research and Development Program of China (2012AA10A307), the Agricultural Science Creation in Henan Province, and the Modern Agricultural System in Industry and Technology of Henan Province, China (S2010- 02-G01).

QTL consistency for agronomic traits across three generations and potential applications in popcorn

DONG Yong-bin, ZHANG Zhong-wei, SHI Qing-ling, WANG Qi-lei, ZHOU Qiang, DENG Fei, MA Zhi-yan, QIAO Da-he, LI Yu-ling

College of Agronomy, Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops/National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, P.R.China

Received:2014-12-03 Online:2015-12-01 Published:2015-12-16
Contact: LI Yu-ling, Tel: +86-371-63555540,Fax: +86-371-63558126, E-mail: yuling_li@126.com
About author:DONG Yong-bin, Tel: +86-371-63555540, E-mail: dyb0816@163.com;
Supported by:
This work was funded by the Plan for the Scientific Innovation Talent of Henan Province, China (124200510003), the National High-Tech Research and Development Program of China (2012AA10A307), the Agricultural Science Creation in Henan Province, and the Modern Agricultural System in Industry and Technology of Henan Province, China (S2010- 02-G01).

摘要/Abstract

摘要： Favorable agronomic traits are important to improve productivity of popcorn. In this study, a recombinant inbred line (RIL) population consisting of 258 lines was evaluated to identify quantitative trait loci (QTLs) for nine agronomic traits (plant height, ear height, top height (plant height subtracted ear height), top height/plant height, number of leaves above the top ear, leaf area, stalk diameter, number of tassel branches and the length of tassel) under three environments. Meta-analysis was conducted then to integrate QTLs identified across three generations (RIL, F2:3 and BC2F2) developed from the same crosses. In total, 179 QTLs and 36 meta-QTLs (mQTL) were identified. The percentage of phenotypic variation (R2) explained by any single QTL varied from 3.86 to 28.4%, and 24 QTLs with contributions over 15%. Nine common QTLs located in the same or similar chromosome regions were detected across three generations. Five meta-QTLs were identified including QTLs in three independent studies. Seven important mQTLs were composed of 11–26 QTLs for 4–7 traits, respectively. Only 11 mQTLs were commonly identified in the same or similar chromosome regions across agronomic traits, popping characteristics (popping fold, popping volume and popping rate) and grain yield components (ear weight per plant, grain weight per plant, 100-grain weight, ear length, kernel number per row, ear diameter, row number per ear and kernel ratio) by meta-QTL analysis. In conclusion, we identified a list of QTLs, some of which with much higher contributions to agronomic traits should be valuable for further study in improving both popping characteristics and grain yield components in popcorn.

关键词: popcorn , agronomic traits , quantitative trait locus (QTL) , meta-analysis

Abstract: Favorable agronomic traits are important to improve productivity of popcorn. In this study, a recombinant inbred line (RIL) population consisting of 258 lines was evaluated to identify quantitative trait loci (QTLs) for nine agronomic traits (plant height, ear height, top height (plant height subtracted ear height), top height/plant height, number of leaves above the top ear, leaf area, stalk diameter, number of tassel branches and the length of tassel) under three environments. Meta-analysis was conducted then to integrate QTLs identified across three generations (RIL, F2:3 and BC2F2) developed from the same crosses. In total, 179 QTLs and 36 meta-QTLs (mQTL) were identified. The percentage of phenotypic variation (R2) explained by any single QTL varied from 3.86 to 28.4%, and 24 QTLs with contributions over 15%. Nine common QTLs located in the same or similar chromosome regions were detected across three generations. Five meta-QTLs were identified including QTLs in three independent studies. Seven important mQTLs were composed of 11–26 QTLs for 4–7 traits, respectively. Only 11 mQTLs were commonly identified in the same or similar chromosome regions across agronomic traits, popping characteristics (popping fold, popping volume and popping rate) and grain yield components (ear weight per plant, grain weight per plant, 100-grain weight, ear length, kernel number per row, ear diameter, row number per ear and kernel ratio) by meta-QTL analysis. In conclusion, we identified a list of QTLs, some of which with much higher contributions to agronomic traits should be valuable for further study in improving both popping characteristics and grain yield components in popcorn.

Key words: popcorn , agronomic traits , quantitative trait locus (QTL) , meta-analysis

DONG Yong-bin, ZHANG Zhong-wei, SHI Qing-ling, WANG Qi-lei, ZHOU Qiang, DENG Fei, MA Zhi-yan, QIAO Da-he, LI Yu-ling. QTL consistency for agronomic traits across three generations and potential applications in popcorn[J]. Journal of Integrative Agriculture, 2015, 14(12): 2547-2557.

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QTL consistency for agronomic traits across three generations and potential applications in popcorn

QTL consistency for agronomic traits across three generations and potential applications in popcorn

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