Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (7): 1376-1382.DOI: 10.1016/S2095-3119(14)60976-X

• 论文 • 上一篇    下一篇

Identification of the miniature pig inbred line by skin allograft

 MU Yu-lian, LIU Lan, FENG Shu-tang, WU Tian-wen, LI Kui, LI Jun-you, HE Wei, GAO Qian, ZHOU Wen-fang, WEI Jing-liang, TANG Fang, YANG Shu-lin, WU Zhi-gu, XIA Ying, SUN Tong-zhu   

  1. 1、Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese
    Academy of Agricultural Sciences, Beijing 100193, P.R.China
    2、Key Laboratory of Wound Repair, the General Hospital of the People’s Liberation Army, Beijing 100048, P.R.China
  • 收稿日期:2014-09-30 出版日期:2015-07-03 发布日期:2015-07-06
  • 通讯作者: FENG Shu-tang,E-mail: fst508@sina.com
  • 作者简介:MU Yu-lian, E-mail: muyulian76@iascaas.net.cn; LIU Lan,E-mail: liulan19789@163.com;* These authors contributed equally to this study.
  • 基金资助:

    This work was supported by the National High Technology Research and Development Program of China (2012AA020603), the National Transgenic Major Project, China (2008ZX08012-002-05), and the National Key Technoligy R&D Program of China (2012BA13904).

Identification of the miniature pig inbred line by skin allograft

 MU Yu-lian, LIU Lan, FENG Shu-tang, WU Tian-wen, LI Kui, LI Jun-you, HE Wei, GAO Qian, ZHOU Wen-fang, WEI Jing-liang, TANG Fang, YANG Shu-lin, WU Zhi-gu, XIA Ying, SUN Tong-zhu   

  1. 1、Key Laboratory of Farm Animal Genetic Resources and Utilization, Ministry of Agriculture/Institute of Animal Sciences, Chinese
    Academy of Agricultural Sciences, Beijing 100193, P.R.China
    2、Key Laboratory of Wound Repair, the General Hospital of the People’s Liberation Army, Beijing 100048, P.R.China
  • Received:2014-09-30 Online:2015-07-03 Published:2015-07-06
  • Contact: FENG Shu-tang,E-mail: fst508@sina.com
  • About author:MU Yu-lian, E-mail: muyulian76@iascaas.net.cn; LIU Lan,E-mail: liulan19789@163.com;* These authors contributed equally to this study.
  • Supported by:

    This work was supported by the National High Technology Research and Development Program of China (2012AA020603), the National Transgenic Major Project, China (2008ZX08012-002-05), and the National Key Technoligy R&D Program of China (2012BA13904).

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摘要： Skin grafting has been used as one of the most reliable tests to determine the genetic stability of laboratory animal such as mice and rats inbred line, but no identification of swine inbred lines by skin grafting has been reported. At present, Wuzhishan miniature pig (WZSP) inbred line has acquired the F24 individuals in China. In order to verify whether WZSP inbred line had been cultivated successfully, allogeneic skin grafts and related research were performed on F20 individuals of WZSP inbreeding population, compared with a control group of autologous transplantation. We observed the transplant recipients’ wounds, detected peripheral blood-related indicators interleukin-2, 4 and 10, CD4+ and CD8+ lymphocytes, and conducted hematoxylin-eosin (HE) and Masson’s staining of skin to judge whether the immune rejection reactions occurred within 28 days after transplantation. Chr. 7 genomic heterozygosity of 48 WZSP individuals from F20 to F22 was analyzed by high-density single nucleotide polymorphism (SNP) chips (60 000 SNPs). The result showed that there were no significant differences in graft skin, the plasma interleukin-2, 4, 10, CD4+ and CD8+, HE and Masson’s staining results between the allograft and autograft groups, and no immune rejection occurred on the allograft group. We found that 11 genes in Chr. 7 of major histocompatibility complex (MHC) I and MHC II were homozygous which confirmed that immune antibody of the allograft and autograft groups were highly identical and also provided a theoretical basis to no immune rejection occurred on the allograft in the inbred WZSP. The result proved that the WZSP inbred line had been cultivated successfully for the first time in the world. The test methods also provide a scientific basis for the identification of swine and mammal inbred lines.

关键词: Wuzhishan miniature pigs , inbred line , skin allograft , immune rejection

Abstract: Skin grafting has been used as one of the most reliable tests to determine the genetic stability of laboratory animal such as mice and rats inbred line, but no identification of swine inbred lines by skin grafting has been reported. At present, Wuzhishan miniature pig (WZSP) inbred line has acquired the F24 individuals in China. In order to verify whether WZSP inbred line had been cultivated successfully, allogeneic skin grafts and related research were performed on F20 individuals of WZSP inbreeding population, compared with a control group of autologous transplantation. We observed the transplant recipients’ wounds, detected peripheral blood-related indicators interleukin-2, 4 and 10, CD4+ and CD8+ lymphocytes, and conducted hematoxylin-eosin (HE) and Masson’s staining of skin to judge whether the immune rejection reactions occurred within 28 days after transplantation. Chr. 7 genomic heterozygosity of 48 WZSP individuals from F20 to F22 was analyzed by high-density single nucleotide polymorphism (SNP) chips (60 000 SNPs). The result showed that there were no significant differences in graft skin, the plasma interleukin-2, 4, 10, CD4+ and CD8+, HE and Masson’s staining results between the allograft and autograft groups, and no immune rejection occurred on the allograft group. We found that 11 genes in Chr. 7 of major histocompatibility complex (MHC) I and MHC II were homozygous which confirmed that immune antibody of the allograft and autograft groups were highly identical and also provided a theoretical basis to no immune rejection occurred on the allograft in the inbred WZSP. The result proved that the WZSP inbred line had been cultivated successfully for the first time in the world. The test methods also provide a scientific basis for the identification of swine and mammal inbred lines.

Key words: Wuzhishan miniature pigs , inbred line , skin allograft , immune rejection