贝莱斯芽孢杆菌接种对熏蒸处理的连作障碍土壤中外源有机碳矿化及细菌群落组成的影响

doi:10.1016/j.jia.2025.12.076

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (9): 3904-3916.DOI: 10.1016/j.jia.2025.12.076

贝莱斯芽孢杆菌接种对熏蒸处理的连作障碍土壤中外源有机碳矿化及细菌群落组成的影响

收稿日期:2026-08-08 修回日期:2026-01-02 接受日期:2025-12-12 出版日期:2026-09-20 发布日期:2026-08-11

Impacts of Bacillus velezensis inoculation on exogenous organic carbon mineralization and bacterial community composition in fumigated continuous-cropping obstacle soils

Yixian Liu^{1, 2}, Runa Zhang^{1, 2}, Shuai Ding^{1, 2#}, Shuang Wang^{1, 2}, Liang Wei^{1, 2}, Cuiyan Wu³, Wensheng Fang⁴, Qiuxia Wang⁴, Dongdong Yan⁴, Aocheng Cao⁴, Jianping Chen^{1, 2}, Tida Ge^{1, 2}, Zhenke Zhu^{1, 2#}

¹State Key Laboratory for Quality and Safety of Agro-products/Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs/Key Laboratory of Green Plant Protection of Zhejiang Province/Institute of Plant Virology, Ningbo University, Ningbo 315211, China
²International Science and Technology Cooperation Base for the Regulation of Soil Biological Functions and One Health of Zhejiang Province, Ningbo University, Ningbo 315211, China
³School of Materials and Chemical Engineering, Ningbo University of Technology, Ningbo 315211, China
⁴Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2026-08-08 Revised:2026-01-02 Accepted:2025-12-12 Online:2026-09-20 Published:2026-08-11
About author:Yixian Li, E-mail: 2311130026@nbu.edu.cn; #Correspondence Shuai Ding, E-mail: dingshuai@nbu.edu.cn; Zhenke Zhu, E-mail: zhuzhenke@nbu.edu.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2023YFD1701200), the Beijing Natural Science Foundation, China (6232034).

摘要/Abstract

摘要：

化学熏蒸剂如棉隆(DZ)和二甲基二硫(DMDS)能够有效抑制土传病害，但熏蒸后的连作障碍土壤生态功能恢复存在不确定性，例如由微生物介导的有机碳循环。然而，微生物修复措施如何增强熏蒸后连作土壤微生物碳矿化活性的作用机制仍不明确。本研究通过土壤培养实验，探究了接种贝莱斯芽孢杆菌(Bacillus velezensis)对连作障碍土壤熏蒸后外源有机碳(EOC)矿化以及细菌群落组成和互作的影响。与化学熏蒸处理相比，额外接种B.velezensis使DZ熏蒸处理土壤的EOC累积矿化量提高了27%，DMDS熏蒸处理土壤提高了22%。贝莱斯芽孢杆菌接种促进外源碳矿化主要是因为核心类群和关键种的富集，增强了土壤微生物碳代谢活性。结构方程模型分析进一步表明，核心微生物类群(如OTU56，属于Bacillus)能够与土著细菌产生积极互作，从而促进EOC 矿化。研究表明B.velezensis通过选择性地重塑细菌群落并增强细菌协作网络，促进了熏蒸后连作障碍土壤微生物碳代谢功能的快速恢复。本研究为化学熏蒸后连作障碍土壤生态功能恢复提供了理论支撑，并为化学胁迫农田生态系统发展可持续管理措施提供了科学依据。

Abstract:

Chemical fumigants such as dazomet (DZ) and dimethyl disulfide (DMDS) effectively suppress soil-borne pathogens but there is uncertainty regarding the restoration of soil ecological functions in continuous cropping obstacles after fumigation, such as microbe-mediated organic carbon cycling. However, the mechanism by which microbial remediation measures enhance carbon mineralization activity after soil fumigation remains unclear. In this study, we conducted microcosm experiments to investigate the impacts of Bacillus velezensis inoculation on exogenous organic carbon (EOC) mineralization and bacterial community composition and interactions following chemical fumigation. Relative to fumigation alone, B. velezensis addition increased cumulative EOC mineralization by 27% in DZ-treated soils and by 22% in DMDS-treated soils. This enhancement was associated with the enrichment of core taxa and keystone species, which collectively increased microbial activity. Structural equation modeling further confirmed that core taxa (OTU56, belonging to Bacillus) induced positive interactions with indigenous species, which drove the observed enhancement in EOC mineralization. We conclude that B. velezensis facilitates the rapid recovery of soil carbon mineralization after fumigation by selectively reshaping the bacterial community and strengthening bacterial cooperative networks. This work provides a mechanistic framework for microbially driven ecological restoration of fumigant-impacted continuous-cropping obstacle soils and informs the development of sustainable soil-management practices in chemically challenged agroecosystems.

Key words: continuous cropping obstacles , chemical fumigation , B. velezensis , microbial community structure , organic carbon mineralization

Yixian Liu, Runa Zhang, Shuai Ding, Shuang Wang, Liang Wei, Cuiyan Wu, Wensheng Fang, Qiuxia Wang, Dongdong Yan, Aocheng Cao, Jianping Chen, Tida Ge, Zhenke Zhu. 贝莱斯芽孢杆菌接种对熏蒸处理的连作障碍土壤中外源有机碳矿化及细菌群落组成的影响[J]. Journal of Integrative Agriculture, 2026, 25(9): 3904-3916.

Yixian Liu, Runa Zhang, Shuai Ding, Shuang Wang, Liang Wei, Cuiyan Wu, Wensheng Fang, Qiuxia Wang, Dongdong Yan, Aocheng Cao, Jianping Chen, Tida Ge, Zhenke Zhu. Impacts of Bacillus velezensis inoculation on exogenous organic carbon mineralization and bacterial community composition in fumigated continuous-cropping obstacle soils[J]. Journal of Integrative Agriculture, 2026, 25(9): 3904-3916.

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贝莱斯芽孢杆菌接种对熏蒸处理的连作障碍土壤中外源有机碳矿化及细菌群落组成的影响

Impacts of Bacillus velezensis inoculation on exogenous organic carbon mineralization and bacterial community composition in fumigated continuous-cropping obstacle soils

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