全球NADC34-like猪繁殖与呼吸综合征病毒的遗传多样性与重组演化模式分析

doi:10.1016/j.jia.2026.01.006

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (8): 3503-3507.DOI: 10.1016/j.jia.2026.01.006

全球NADC34-like猪繁殖与呼吸综合征病毒的遗传多样性与重组演化模式分析

收稿日期:2025-08-23 修回日期:2026-01-07 接受日期:2025-12-23 出版日期:2026-08-20 发布日期:2026-07-09

Genetic diversity and recombination analysis of NADC34-like porcine reproductive and respiratory syndrome viruses

Zhengqin Ye¹, Wenqiang Wang¹, Zhenbang Zhu¹, Wei Wen¹, Hu Suk Lee^2,³, Xiangdong Li¹^#

¹Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
²International Livestock Research Institute, Hanoi 112000, Vietnam
³College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea

Received:2025-08-23 Revised:2026-01-07 Accepted:2025-12-23 Online:2026-08-20 Published:2026-07-09
About author:Zhengqin Ye, E-mail: yezhengqin@126.com; Wenqiang Wang, E-mail: wqwang@yzu.edu.cn; Zhenbang Zhu, E-mail: 007583@yzu.edu.cn; Wei Wen, E-mail: 008634@yzu.edu.cn; Hu Suk Lee, E-mail: hs.lee@cnu.ac.kr; #Correspondence Xiangdong Li, E-mail: 007352@yzu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China-CGIAR (32361143511), the Jiangsu Innovative and Entrepreneurial Talent Team Project, China (JSSCTD202224), the 111 Project D18007, and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).

摘要/Abstract

摘要：

猪繁殖与呼吸综合征（PRRS）是威胁全球养猪业的重要疫病。近年来，NADC34-like毒株因其复杂的重组背景和多变的致病性备受关注。本研究旨在通过全球视野下的全基因组分析，明确NADC34-like毒株的遗传多样性、时空分布特征及重组规律，为该毒株的精准防控提供理论依据。本研究从GenBank数据库中检索并筛选了截至2025年6月的1,598条PRRSV全基因组序列，最终聚焦于来自中国、美国和韩国的176条NADC34-like毒株序列。利用IQ-TREE软件构建最大似然系统发育树进行分类研究；采用RDP4（集成7种算法）及Simplot软件对重组事件进行系统鉴定与验证；并结合时间与地理信息，分析了不同国家毒株重组热点的演化趋势。遗传分类显示，NADC34-like毒株内平均核苷酸距离为5.68%，而与其他谱系距离均超过14.89%。重组分析发现，在176条毒株中，重组毒株占比56.82%（100/176），已成为该亚群的主要存在形式。研究界定了12种重组模式（R1–R12），其中NADC34-R1是美国最流行的模式，且具有明显的地域聚集性。发现中国NADC34-like毒株的重组热点存在显著的时空转移：2017-2019年重组多发生于结构蛋白区（ORFs 2-7），而2020年后则主要集中在ORF1a区域，这与美国和韩国毒株持续集中于ORF1ab的特征显著不同。谱系1.8（Lineage 1.8）被鉴定为全球范围内最主要的次要亲本贡献者（占比54.84%），是驱动该毒株遗传多样化的核心引擎。NADC34-like PRRSV在全球范围内呈现高度的遗传多样性和复杂的区域演化特征。虽然重组模式与毒株毒力间的直接联系尚待明确，但重组株的广泛流行及其演化热点的漂移提示该毒株具有极强的环境适应性。首次在全球尺度下对NADC34-like毒株进行了系统的全基因组重组模式分类，确立了R1-R12评价框架。揭示了中国毒株重组热点从结构基因向非结构基因区转移的独特演化规律。明确了Lineage 1.8作为“通用亲本”在驱动全球NADC34-like毒株多样性中的关键作用。

Zhengqin Ye, Wenqiang Wang, Zhenbang Zhu, Wei Wen, Hu Suk Lee, Xiangdong Li. 全球NADC34-like猪繁殖与呼吸综合征病毒的遗传多样性与重组演化模式分析[J]. Journal of Integrative Agriculture, 2026, 25(8): 3503-3507.

Zhengqin Ye, Wenqiang Wang, Zhenbang Zhu, Wei Wen, Hu Suk Lee, Xiangdong Li. Genetic diversity and recombination analysis of NADC34-like porcine reproductive and respiratory syndrome viruses[J]. Journal of Integrative Agriculture, 2026, 25(8): 3503-3507.

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全球NADC34-like猪繁殖与呼吸综合征病毒的遗传多样性与重组演化模式分析

Genetic diversity and recombination analysis of NADC34-like porcine reproductive and respiratory syndrome viruses

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