Engineered ferritin nanocages for efficient siRNA delivery and potent gene silencing in plants

doi:10.1016/j.jia.2026.05.063

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (8): 3498-3502.DOI: 10.1016/j.jia.2026.05.063

收稿日期:2026-02-09 修回日期:2026-05-30 接受日期:2026-04-17 出版日期:2026-08-20 发布日期:2026-07-09

Engineered ferritin nanocages for efficient siRNA delivery and potent gene silencing in plants

Han Zhang^{1, 3*}, Ziyan Hu^2*, Xiangyu Bao^1*, Kun Wang¹, Lijia Zheng⁴, Changqing Zhang³, Xueyong Yang^1#, Jiachen Zang^2#, Yuxuan Qin^1#

¹ State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
² College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
³College of Horticulture, China Agricultural University, Beijing 100193, China
⁴Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China

Received:2026-02-09 Revised:2026-05-30 Accepted:2026-04-17 Online:2026-08-20 Published:2026-07-09
About author: #Correspondence Xueyong Yang, E-mail: yangxueyong@caas.cn; Jiachen Zang, E-mail: zangjiachen@cau.edu.cn; Yuxuan Qin, E-mail: qinyuxuan@caas.cn * These authors contributed equally to this study.
Supported by:
This work was supported by the Beijing Rural Revitalization Agricultural Science and Technology Project, China (NY2601500000 and Z231100003723005), the National Natural Science Foundation of China (NSFC) (32525052), and the Agricultural Science and Technology Innovation Program (ASTIP) of the Chinese Academy of Agricultural Sciences (CAAS-CSIAF-202401, CAAS-ZDRW202103, and CAAS-ASTIP-2024-IVF).

摘要/Abstract

Han Zhang, Ziyan Hu, Xiangyu Bao, Kun Wang, Lijia Zheng, Changqing Zhang, Xueyong Yang, Jiachen Zang, Yuxuan Qin. [J]. Journal of Integrative Agriculture, 2026, 25(8): 3498-3502.

Han Zhang, Ziyan Hu, Xiangyu Bao, Kun Wang, Lijia Zheng, Changqing Zhang, Xueyong Yang, Jiachen Zang, Yuxuan Qin. Engineered ferritin nanocages for efficient siRNA delivery and potent gene silencing in plants[J]. Journal of Integrative Agriculture, 2026, 25(8): 3498-3502.

参考文献

Chen H, Xie J, Hu M, Han X, Fu Y, Dai H, Ma L, Zhang Y. 20 23. In vivo biosynthesis of nutritional holoferritin nanoparticles: Preparation, characterization, iron content analysis, and synthetic pathway. Food Chemistry, 414, 135692.

Cheng X, Zhou Q, Xiao J, Qin X, Zhang Y, Li X, Zheng W, Zhang H. 20 24. Nanoparticle LDH enhances RNAi efficiency of dsRNA in piercing-sucking pests by promoting dsRNA stability and transport in plants. Journal of Nanobiotechnology, 22, 544.

Ding C, Jiao Y, Li Y, Shen L, Zhang W, Ma H, Han L, Zhang Z, Yan F, Mei Y. 2024. A novel pH-responsive multi-component nanodelivery system with siRNA for plant disease management. Chemical Engineering Journal, 497, 155001.

Dumančić E, Vojta L, Fulgosi H. 2023. Beginners guide to sample preparation techniques for transmission electron microscopy. Periodicum Biologorum, 125, 123–131.

Goralogia G S, Willig C, Strauss S H. 2025. Engineering Agrobacterium for improved plant transformation. The Plant Journal, 121, e70015.

Jin Y, Zhang B, Li J, Guo Z, Zhang C, Chen X, Ma L, Wang Z, Yang H, Li Y. 2025. Bioengineered protein nanocarrier facilitating siRNA escape from lysosomes for targeted RNAi therapy in glioblastoma. Science Advances, 11, eadr9266.

Kandhol N, Dash P K, Singh V P, Deshmukh R, Dhankher O P, Tran L S P, White J C, Tripathi D K. 2025. Nanomaterial based gene delivery in plants: An upcoming genetic revolution? Trends in Plant Science, 30, 1251–1261.

Lee E J, Lee S J, Kang Y S, Ryu J H, Kwon K C, Jo E, Yhee J Y, Kwon I C, Kim K, Lee J. 2015. Engineered proteinticles for targeted delivery of siRNA to cancer cells. Advanced Functional Materials, 25, 1279–1286.

Ming X, Wan M C, Zhang Z D, Xue H C, Wu Y Q, Xu Z G, Lian H, Yuan M T, Mai Y X, Hu Y X, Liu K, Gao J, Shao Q L, Marchant D B, Nelms B, Walbot V, Wang J W. 2025. FX-Cell: A method for single-cell RNA sequencing on difficult-to-digest and cryopreserved plant samples. Nature Methods, 22, 2551–2562.

Qi J, Gu J, Xu W, Li Y, Xu G, Xi X, Xie Z, Li Z, Wu H. 2026. Leaf cuticle and plasma membrane are critical for a Better Increase of cotton salinity stress tolerance by l-than d-Gold Nanoclusters. American Chemical Society Nano, 20, 1598–1613.

Qi J, Li Y, Yao X, Li G, Xu W, Chen L, Xie Z, Gu J, Wu H, Li Z. 2024. Rational design of ROS scavenging and fluorescent gold nanoparticles to deliver siRNA to improve plant resistance to Pseudomonas syringae. Journal of Nanobiotechnology, 22, 446.

Qiao J, Qi L. 2021. Recent progress in plant-gold nanoparticles fabrication methods and bio-applications. Talanta, 223, 121396.

Raza A, Zaman Q U, Shabala S, Tester M, Munns R, Hu Z, Varshney R K. 2025. Genomics-assisted breeding for designing salinity-smart future crops. Plant Biotechnology Journal, 23, 3119–3151.

Santana I, Jeon S J, Kim H I, Islam M R, Castillo C, Garcia G F H, Newkirk G M, Giraldo J P. 2022. Targeted carbon nanostructures for chemical and gene delivery to plant chloroplasts. American Chemical Society Nano, 16, 12156–12173.

Shen D, Rajappa S, Zhao Y, Pang Z, Li Y, Thenarianto C, Puangpathumanond S, Sevencan C, Lew T T S. 2025. Amphibious microneedles for programmable delivery of biomolecules and microorganisms in living plants. Nature Communications, 16, 10993.

Wang C, Li Z, Liu B, Liao Q, Bao C, Fu H, Pan B, Jin W, Cui D. 2013. Dendrimer modified SWCNTs for high efficient delivery and intracellular imaging of survivin siRNA. Nano Biomedicine & Engineering, 5, 131–136.

Wang P, Si H, Li C, Xu Z, Guo H, Jin S, Cheng H. 2025. Plant genetic transformation: Achievements, current status and future prospects. Plant Biotechnology Journal, 23, 2034–2058.

Wu J, Zeng X, Wang L, Zhang J, Cui S, Ma Z, Pan R, Liu C, Kong D, Song J, Liu L, Feng C, Shen W, Suo X, Lin Z, Lee H K, Tang S. 2025. Embedding carbon nanotubes in artificial cells enhances probe transfer. Advanced Materials, 37, e2418271.

Yang J, Li Y, Jiang S, Tian Y, Zhang M, Guo S, Wu P, Li J, Xu L, Li W. 2025. Engineered brain‐targeting exosome for reprogramming immunosuppressive microenvironment of glioblastoma. Exploration, 5, 20240039.

Yong J, Xu W, Wu M, Zhang R, Mann C W G, Liu G, Brosnan C A, Mitter N, Carroll B J, Xu Z P. 2025. Lysozyme-coated nanoparticles for active uptake and delivery of synthetic RNA and plasmid-encoded genes in plants. Nature Plants, 11, 131–144.

Zhang H, Cao Y, Xu D, Goh N S, Demirer G S, CestellosBlanco S, Chen Y, Landry M P, Yang P. 2021. Gold-nanocluster-mediated delivery of siRNA to intact plant cells for efficient gene knockdown. Nano Letters, 21, 5859–5866.

Zheng M, Tao W, Zou Y, Farokhzad O C, Shi B. 2018. Nanotechnology-based strategies for siRNA brain delivery for disease therapy. Trends in Biotechnology, 36, 562–575.

Zhu C, Mu J, Liang L. 2024. Nanocarriers for intracellular delivery of proteins in biomedical applications: Strategies and recent advances. Journal of Nanobiotechnology, 22, 688.

Engineered ferritin nanocages for efficient siRNA delivery and potent gene silencing in plants

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics