番茄NBS-LRR基因Mi-9介导高温稳定的根结线虫抗性

doi:10.1016/j.jia.2024.07.017

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (7): 2869-2875.DOI: 10.1016/j.jia.2024.07.017

番茄NBS-LRR基因Mi-9介导高温稳定的根结线虫抗性

收稿日期:2024-02-18 修回日期:2024-07-18 接受日期:2024-06-11 出版日期:2025-07-20 发布日期:2025-06-17

A tomato NBS-LRR gene Mi-9 confers heat-stable resistance to root-knot nematodes

Shudong Chen^{1, 2, 3*}, Yupan Zou^{1, 3*}, Xin Tong^{1, 2, 3}, Cao Xu^{1, 3#}

¹Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
²University of Chinese Academy of Sciences, Beijing 100049, China
³CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Received:2024-02-18 Revised:2024-07-18 Accepted:2024-06-11 Online:2025-07-20 Published:2025-06-17
About author:#Correspondence Cao Xu, E-mail: caoxu@genetics.ac.cn * These authors contributed equally to this study.
Supported by:
This work was supported by the National Key R&D Program of China (2018YFA0900600 and 2021YFF1000103-5), and the Strategic Priority Research Program of Chinese Academy of Sciences (XDA24030503).

摘要/Abstract

摘要：

根结线虫(RKNs)是土壤传播最为广泛的植物内寄生虫。它们会感染众多作物的根部，造成严重的产量损失。在番茄中，唯一商业化的抗根结线虫基因Mi-1.2在土壤温度超过28°C时失效。我们从秘鲁番茄小种LA2157中克隆了热稳定的抗根结线虫基因Mi-9，该基因存在于一段由七个NBS-LRR抗性基因组成的基因簇中。通过单独和组合敲除多个候选基因，我们发现单个候选基因Mi-9 Candidate-4 (MiC-4)就足以产生热稳定RKN抗性。我们的研究为番茄在极端高温频发的种植环境下获得热稳定根结线虫抗性找到了新的基因资源。我们还绘制了通过结合比较基因组学和基因组编辑手段快速鉴定抗性基因的路线图，或可用于多种作物遗传改良。

Abstract:

Root-knot nematodes (RKNs) are the most widespread soil-borne obligate endoparasites. They can infect the roots of many crops and cause significant yield losses. The only commercially available RKN-resistant gene in tomatoes, Mi-1.2, fails at soil temperatures above 28°C. We cloned the heat-stable RKN-resistant gene, Mi-9, from a gene cluster composed of seven nucleotide-binding sites and leucine-rich repeat (NBS-LRR) type resistant genes in Solanum arcunum accession LA2157. Screening nematode infections in individual and combinatorial knockouts of five NBS-LRR genes showed that Mi-9 Candidate 4 (MiC-4) alone is sufficient to confer heat-stable RKN resistance. Our study identifies a new source of heat-stable resistance to RKN in tomatoes for challenging environmental conditions. We also showcase a roadmap for rapid characterization of resistance genes by combining comparative genomics and genome editing, with the potential to be utilized in other crops.

Key words: tomato , nematode , heat-stable resistance , Mi-9 , genome editing

Shudong Chen, Yupan Zou, Xin Tong, Cao Xu. 番茄NBS-LRR基因Mi-9介导高温稳定的根结线虫抗性[J]. Journal of Integrative Agriculture, 2025, 24(7): 2869-2875.

Shudong Chen, Yupan Zou, Xin Tong, Cao Xu. A tomato NBS-LRR gene Mi-9 confers heat-stable resistance to root-knot nematodes[J]. Journal of Integrative Agriculture, 2025, 24(7): 2869-2875.

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番茄NBS-LRR基因Mi-9介导高温稳定的根结线虫抗性

A tomato NBS-LRR gene Mi-9 confers heat-stable resistance to root-knot nematodes

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