现代育种选择基因ZmMYC2调控玉米生长和抗逆基因表达的研究

doi:10.1016/j.jia.2024.12.020

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (7): 2876-2880.DOI: 10.1016/j.jia.2024.12.020

现代育种选择基因ZmMYC2调控玉米生长和抗逆基因表达的研究

收稿日期:2024-07-29 修回日期:2024-12-19 接受日期:2024-11-12 出版日期:2025-07-20 发布日期:2025-06-17

ZmMYC2, selected during modern breeding, orchestrates growth and defense gene expression in maize

Shuai Ma¹, Xiangyu Lu², Bo Zhou³, Jiameng Zhu^{2, 4}, Qianhe Zhang^{2, 5}, Suzhen Li², Xiaoqing Liu², Wenzhu Yang², Chunhui Li¹, Yongxiang Li¹, Yu Li¹, Rumei Chen^2#, Xiaojin Zhou^2#, Tianyu Wang^1#

¹Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
²Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
³Institute of Cereal Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
⁴Jiangsu Key Laboratory of Biodiversity and Biotechnology/College of Life Sciences, Nanjing Normal University, Nanjing 210023, China
⁵Department of Environmental Systems Science, Swiss Federal Institute of Technology Zurich (ETHZ), Zurich 8092, Switzerland

Received:2024-07-29 Revised:2024-12-19 Accepted:2024-11-12 Online:2025-07-20 Published:2025-06-17
About author:#Correspondence Rumei Chen, E-mail: chenrumei@caas.cn; Xiaojin Zhou, E-mail: zhouxiaojin@caas.cn; Tianyu Wang, E-mail: wangtianyu@caas.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2023YFD1200503 to Shuai Ma and 2021YFD1200700 to Tianyu Wang).

摘要/Abstract

摘要：

玉米是我国第一大作物，稳定其生长和抗逆的平衡是保障高产稳产的重要基础。茉莉素在植物发育和应对逆境胁迫中发挥重要功能，该信号通路中的核心转录因子MYC2调控多种发育及逆境相关基因表达。虽然MYC2在拟南芥中的功能已经得到广泛研究，但其在玉米中的表达调控网络解析尚不够全面。本研究以玉米为研究对象，首先明确了ZmMYC2在玉米育种进程中受到选择，其启动子区的优异单倍型有利于基因的高表达。借助PER-seq技术和差异表达基因分析筛选得到多个ZmMYC2的候选下游基因；根据功能注释可将这些基因分为：抗逆相关基因（ZmCYP709H1、ZmBX5、ZmBX6）和生长发育相关基因（ZmBRD1、ZmTIP3c、ZmARF3、ZmCER2）；进而利用eQTL分析、EMSA和双荧光素酶报告基因等实验，验证了ZmMYC2能够直接激活上述靶基因的表达，拓展了ZmMYC2调控玉米逆境响应和生长发育相关基因表达的网络，为玉米高产和抗逆的协同改良提供理论依据和基因资源。

Shuai Ma, Xiangyu Lu, Bo Zhou, Jiameng Zhu, Qianhe Zhang, Suzhen Li, Xiaoqing Liu, Wenzhu Yang, Chunhui Li, Yongxiang Li, Yu Li, Rumei Chen, Xiaojin Zhou, Tianyu Wang. 现代育种选择基因ZmMYC2调控玉米生长和抗逆基因表达的研究[J]. Journal of Integrative Agriculture, 2025, 24(7): 2876-2880.

Shuai Ma, Xiangyu Lu, Bo Zhou, Jiameng Zhu, Qianhe Zhang, Suzhen Li, Xiaoqing Liu, Wenzhu Yang, Chunhui Li, Yongxiang Li, Yu Li, Rumei Chen, Xiaojin Zhou, Tianyu Wang. ZmMYC2, selected during modern breeding, orchestrates growth and defense gene expression in maize[J]. Journal of Integrative Agriculture, 2025, 24(7): 2876-2880.

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现代育种选择基因ZmMYC2调控玉米生长和抗逆基因表达的研究

ZmMYC2, selected during modern breeding, orchestrates growth and defense gene expression in maize

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