茶树炭疽病抗性遗传位点的QTL定位与候选基因分析

doi:10.1016/j.jia.2025.02.015

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (6): 2240-2250.DOI: 10.1016/j.jia.2025.02.015

茶树炭疽病抗性遗传位点的QTL定位与候选基因分析

收稿日期:2024-01-26 修回日期:2025-02-17 接受日期:2025-01-20 出版日期:2025-06-20 发布日期:2025-05-12

QTL detection and candidate gene analysis of the anthracnose resistance locus in tea plant (Camellia sinensis)

Chenyu Zhang^1*, Hongli Li^2*, Piao Mei¹, Yuanyuan Ye¹, Dingding Liu¹, Yang Gong¹, Haoran Liu¹, Mingzhe Yao^1#, Chunlei Ma^1#

¹Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs/Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
²Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2024-01-26 Revised:2025-02-17 Accepted:2025-01-20 Online:2025-06-20 Published:2025-05-12
About author:Chenyu Zhang, E-mail: zhangchenyu@tricaas.com; Hongli Li, E-mail: lihongli900@126.com; #Correspondence Mingzhe Yao, E-mail: yaomz@tricaas.com; Chunlei Ma, E-mail: malei220@tricaas.com * These authors contributed equally to this study.
Supported by:
This work was supported by Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding-Tea Plant, China (2021C02067-6), the National Key Research and Development Program of China (2021YFD1200200), the Fundamental Research Fund for Tea Research Institute of the Chinese Academy of Agricultural Sciences (1610212022009), and the Zhejiang Provincial Natural Science Foundation, China (Z24C160010) to Chunlei Ma.

摘要/Abstract

摘要：

炭疽病是一种由刺盘孢属（Colletotrichum）引起的重要病害，对茶树（Camellia sinensis）的产量和经济价值有着显著影响。然而，关于茶树炭疽病抗性的遗传机理目前鲜有报道。前期田间调查发现茶树‘龙井43’×‘白鸡冠’杂交F₁代对炭疽病存在明显抗性差异。因此，本研究以‘龙井43’×‘白鸡冠’杂交群体为试材，对茶树炭疽病抗性遗传位点进行了探究。基于连锁分析发现一个主效QTL（qAR-12.4）在两年分别可以解释10.8%和12.7%的表型变异；进一步通过混池测序定位了两个基因组区段与炭疽病抗性具有很强的相关性：位于1号染色体的qARChr1和13号染色体的qARChr13。对亲本‘龙井43’和‘白鸡冠’在接种山茶炭疽菌0、24和48小时后的叶片分别进行转录组测序，结果显示位于qARChr1区段内的核定位ERF转录因子（CsERF105）表达水平在‘白鸡冠’叶片接种后逐渐提高，而在‘龙井43’中则没有该趋势。通过对‘白鸡冠’进行CsERF105反义寡核苷酸处理，发现其炭疽病抗性显著降低，表明CsERF105可能是一个影响炭疽病抗性的正调控因子。综上，本研究初步明确了茶树抗炭疽病的遗传位点，并为后续的分子育种目标提供了理论依据。

Abstract:

Anthracnose is a devastating disease caused by Colletotrichum that significantly affects the yield and economic value of the tea plant (Camellia sinensis). However, few studies have addressed the genetic mechanism of anthracnose resistance (AR). This study investigated the QTL associated with AR in a ‘Longjing 43’×‘Baijiguan’ (LJ43×BJG) population. The field surveys conducted in this study led to the identification of several QTLs for AR on the linkage map. One major QTL (qAR-12.4) accounted for 12% of the phenotypic variance explained over two years. The BSA-seq results also revealed two genomic regions, qARChr1 on chromosome 1 and qARChr13 on chromosome 13, which showed strong correlations with AR. Time-course RNA-seq was performed on LJ43 and BJG inoculated with anthracnose at 0, 24, and 48 hours to screen for candidate genes. The results showed the gradual post-inoculation expression of a nuclear-localized ERF transcription factor (CsERF105) within the qARChr1 locus in BJG but not in LJ43. The AR of BJG was significantly reduced after feeding with CsERF105-specific antisense oligonucleotides, suggesting that CsERF105 may be a positive regulator. The findings of this study add to our general knowledge of the genetic factors involved in the tea plant’s AR and potential breeding targets.

Key words: Camellia sinensis , anthracnose , genetic map , CsERF105 , disease resistance

Chenyu Zhang, Hongli Li, Piao Mei, Yuanyuan Ye, Dingding Liu, Yang Gong, Haoran Liu, Mingzhe Yao, Chunlei Ma. 茶树炭疽病抗性遗传位点的QTL定位与候选基因分析[J]. Journal of Integrative Agriculture, 2025, 24(6): 2240-2250.

Chenyu Zhang, Hongli Li, Piao Mei, Yuanyuan Ye, Dingding Liu, Yang Gong, Haoran Liu, Mingzhe Yao, Chunlei Ma. QTL detection and candidate gene analysis of the anthracnose resistance locus in tea plant (Camellia sinensis)[J]. Journal of Integrative Agriculture, 2025, 24(6): 2240-2250.

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茶树炭疽病抗性遗传位点的QTL定位与候选基因分析

QTL detection and candidate gene analysis of the anthracnose resistance locus in tea plant (Camellia sinensis)

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