栽培花生Arahy12和Arahy16染色体的含油量QTL定位及其KASP标记开发

doi:10.1016/j.jia.2023.11.010

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (6): 2096-2105.DOI: 10.1016/j.jia.2023.11.010

栽培花生Arahy12和Arahy16染色体的含油量QTL定位及其KASP标记开发

收稿日期:2023-08-17 修回日期:2023-11-10 接受日期:2023-09-28 出版日期:2025-06-20 发布日期:2025-05-12

Identification of oil content QTLs on Arahy12 and Arahy16, and development of KASP markers in cultivated peanut (Arachis hypogaea L.)

Bingyan Huang¹, Hua Liu¹, Yuanjin Fang^{1, 2}, Lijuan Miao¹, Li Qin¹, Ziqi Sun¹, Feiyan Qi¹, Lei Chen³, Fengye Zhang³, Shuanzhu Li⁴, Qinghuan Zheng⁴, Lei Shi¹, Jihua Wu³, Wenzhao Dong¹, Xinyou Zhang^1#

¹Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences/Shengnong Laboratory, Henan Province/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou 450002, China
²College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
³Shangqiu Academy of Agriculture and Forestry, Shangqiu 476000, China
⁴Nanyang Academy of Agricultural Sciences, Nanyang 473008, China

Received:2023-08-17 Revised:2023-11-10 Accepted:2023-09-28 Online:2025-06-20 Published:2025-05-12
About author:Bingyan Huang, E-mail: huangbingyan@aliyun.com; #Correspondence Xinyou Zhang, E-mail: haasz@126.com
Supported by:
This work was supported by the National Key R&D Program of China (2022YFD1200400), the earmarked fund for CARS-13, the Major Science and Technology Projects of Henan Province, China (221100110300), and the Henan Provincial R&D Program of Interregional Cooperation for Local Scientific and Technological Development Guided by Central Government, China (YDZX20214100004191).

摘要/Abstract

摘要：

高油高油酸花生品种因其富含营养、货架期长及有益于身体健康而备受消费者、加工企业和农民喜爱。花生含油量和脂肪酸含量性状由多基因控制，在不同遗传背景下鉴定其QTL将有助于分子标记辅助选择或基因组选择，提高花生品质育种效率。我们利用来源于含油量中高及油酸含量差异大的双亲、含有521个家系的RIL群体构建的高密度遗传图谱，以及2年5个环境的花生籽仁品质性状的表型数据，最终定位到两个稳定的含油量性状QTL（qOCAh12.1和qOCAh16.1），并开发了KASP标记在自然群体中得到了验证。QTL区域的单倍型分析表明，qOCAh16.1的高含油量优势单倍型仅存在于少数国外资源。此外，本研究鉴定到的qAh09和qAh19区间含有已知的脂肪酸合成关键酶FAD2基因位点，可同时影响所有7种脂肪酸含量，包括棕榈酸、硬脂酸、油酸、亚油酸、花生酸、花生烯酸和山嵛酸，并鉴定到位于第7染色体的蛋白质和长链饱和脂肪酸含量的主效QTL位点，PVE大于10%。本研究结果将有助于花生含油量的标记辅助选择育种，并为花生籽仁脂肪酸含量变异的遗传基础提供了新的认识。

Abstract:

Peanut kernels rich in oil, particularly those with oleic acid as their primary fatty acid, are in high demand among consumers, the food industry, and farmers due to their superior nutritional content, extended shelf life, and health benefits. The oil content and fatty acid composition are governed by multiple genetic factors. Identifying the quantitative trait loci (QTLs) related to these attributes will facilitate marker-assisted selection and genomic selection, thus enhancing quality-focused peanut breeding programs. For this purpose, we developed a population of 521 recombinant inbred lines (RILs) and tested their kernel quality traits across five different environments. We identified two major and stable QTLs for oil content, qOCAh12.1 and qOCAh16.1. The markers linked to these QTLs were designed by Kompetitive allele-specific PCR (KASP) and subsequently validated. Moreover, we found that the superior haplotype of oil content in the qOCAh16.1 region was conserved within the plant introduction (PI) germplasm cluster, as evidenced by a diverse peanut accession panel. In addition, we determined that qAh09 and qAh19.1, which harbor the key gene encoding fatty acid desaturase 2 (FAD2), influence all seven fatty acids, palmitic, stearic, oleic, linoleic, arachidic, gadoleic, and behenic acids. Regarding the protein content and the long-chain saturated fatty acid behenic acid, qAh07 emerged as the major and stable QTL, accounting for over 10% of the phenotypic variation explained (PVE). These findings can enhance marker-assisted selection in peanut breeding, with the aim of improving the oil content, and deepen our understanding of the genetic mechanisms that shape fatty acid composition.

Key words: peanut , QTL , oil content , fatty acid composition , molecular markers

Bingyan Huang, Hua Liu, Yuanjin Fang, Lijuan Miao, Li Qin, Ziqi Sun, Feiyan Qi, Lei Chen, Fengye Zhang, Shuanzhu Li, Qinghuan Zheng, Lei Shi, Jihua Wu, Wenzhao Dong, Xinyou Zhang. 栽培花生Arahy12和Arahy16染色体的含油量QTL定位及其KASP标记开发[J]. Journal of Integrative Agriculture, 2025, 24(6): 2096-2105.

Bingyan Huang, Hua Liu, Yuanjin Fang, Lijuan Miao, Li Qin, Ziqi Sun, Feiyan Qi, Lei Chen, Fengye Zhang, Shuanzhu Li, Qinghuan Zheng, Lei Shi, Jihua Wu, Wenzhao Dong, Xinyou Zhang. Identification of oil content QTLs on Arahy12 and Arahy16, and development of KASP markers in cultivated peanut (Arachis hypogaea L.)[J]. Journal of Integrative Agriculture, 2025, 24(6): 2096-2105.

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栽培花生Arahy12和Arahy16染色体的含油量QTL定位及其KASP标记开发

Identification of oil content QTLs on Arahy12 and Arahy16, and development of KASP markers in cultivated peanut (Arachis hypogaea L.)

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