转录组和代谢组联合分析揭示人工黑暗条件下‘中油蟠9号’桃果实红色着色机制

doi:10.1016/j.jia.2025.06.011

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (10): 3866-3879.DOI: 10.1016/j.jia.2025.06.011

转录组和代谢组联合分析揭示人工黑暗条件下‘中油蟠9号’桃果实红色着色机制

收稿日期:2024-11-29 修回日期:2025-06-09 接受日期:2025-04-29 出版日期:2025-10-20 发布日期:2025-09-24

Integrated transcriptomic and metabolomic analyses reveal the mechanisms for red fruit coloration in peach cv. ‘Zhongyoupan 9’ under artificial darkness

Tiyu Ding¹, Xinxin Ma¹, Xueli Yu¹, Lirong Wang², Ruijin Zhou¹, Xiaojin Hou¹, Yalin Zhao^1#

¹School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology/Henan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plants/Xinxiang Key Laboratory of Germplasm Resources and Genetic Breeding in Pomology, Xinxiang 453003, China
²Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450000, China

Received:2024-11-29 Revised:2025-06-09 Accepted:2025-04-29 Online:2025-10-20 Published:2025-09-24
About author:Tiyu Ding, E-mail: dingtiyu@hist.edu.cn; #Correspondence Yalin Zhao, E-mail: zhaoyalin@hist.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (32402523), the Science and Technology Program of Henan Province, China (232102111083, 242102111120), and the Natural Science Foundation of Henan Province, China (252300420179).

摘要/Abstract

摘要：

套袋生产的蟠桃越来越受到消费者的喜爱，但套袋抑制多数品种果实花色苷积累和着色。然而，发展迅速的‘中油蟠9号’和其他几个品种能够在套袋条件下积累花色苷，表现为非光依赖型花色苷合成模式，但其中的分子机制尚不明确。本研究以表现为光依赖型花色苷合成模式的‘中蟠17号’和非光依赖型花青素合成模式的‘中油蟠9号’为研究对象，发现‘中油蟠9号’外果皮中的花色苷含量显著高于‘中蟠17号’。代谢组学分析表明，花色苷（特别是花色苷-3-O-葡萄糖苷）的显著增加是黑暗条件下‘中油蟠9号’着红色的直接原因。转录组分析表明，PpHY5（long hypocotyl 5）在‘中油蟠9号’中上调表达，在‘中蟠17号’中下调表达，表达规律与两品种的色泽变化正相关。VIGS介导的PpHY5沉默载体转染桃果实后，花色苷含量显著下降，说明PpHY5正向调控花色苷生物合成。结果表明，人工黑暗条件下，PpHY5正向调控‘中油蟠9号’果皮红色变化。

Abstract:

While bagged flat peaches gain increasing consumer preference, the bagging process inhibits anthocyanin production and fruit coloration in most peach cultivars. Certain peach cultivars, including the rapidly expanding ‘Zhongyoupan 9’, demonstrate a light-independent anthocyanin biosynthesis pattern and accumulate anthocyanins under artificial darkness. The underlying molecular mechanisms, however, remain unelucidated. This study examined two flat peach varieties: ‘Zhongpan 17’, exhibiting light-dependent anthocyanin biosynthesis, and ‘Zhongyoupan 9’, displaying light-independent anthocyanin biosynthesis. The pericarp of ‘Zhongyoupan 9’ contained substantially higher anthocyanin levels compared to ‘Zhongpan 17’. Metabolomic analysis identified the marked increase in anthocyanins, particularly cyanidin-3-O-glucoside, as the primary factor in ‘Zhongyoupan 9’ coloration under artificial darkness. Transcriptomic analyses revealed that PpHY5 (long hypocotyl 5) showed upregulation in ‘Zhongyoupan 9’ but downregulation in ‘Zhongpan 17’, with its expression pattern correlating positively with color changes in both varieties. The role of PpHY5 in positively regulating anthocyanin biosynthesis was confirmed through reduced anthocyanin levels in peach fruits treated with a PpHY5 virus-induced gene-silencing construct. These findings suggest that the PpHY5 regulates red color development in ‘Zhongyoupan 9’ under artificial darkness.

Key words: Zhongyoupan 9 , light independence , anthocyanin , PpHY5 , artificial darkness

Tiyu Ding, Xinxin Ma, Xueli Yu, Lirong Wang, Ruijin Zhou, Xiaojin Hou, Yalin Zhao. 转录组和代谢组联合分析揭示人工黑暗条件下‘中油蟠9号’桃果实红色着色机制[J]. Journal of Integrative Agriculture, 2025, 24(10): 3866-3879.

Tiyu Ding, Xinxin Ma, Xueli Yu, Lirong Wang, Ruijin Zhou, Xiaojin Hou, Yalin Zhao. Integrated transcriptomic and metabolomic analyses reveal the mechanisms for red fruit coloration in peach cv. ‘Zhongyoupan 9’ under artificial darkness[J]. Journal of Integrative Agriculture, 2025, 24(10): 3866-3879.

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转录组和代谢组联合分析揭示人工黑暗条件下‘中油蟠9号’桃果实红色着色机制

Integrated transcriptomic and metabolomic analyses reveal the mechanisms for red fruit coloration in peach cv. ‘Zhongyoupan 9’ under artificial darkness

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