饲粮铁水平对黄羽肉鸡生长、抗氧化、小肠形态及空肠紧密连接蛋白基因表达的影响

doi:10.1016/j.jia.2023.04.041

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (4): 1329-1337.DOI: 10.1016/j.jia.2023.04.041

饲粮铁水平对黄羽肉鸡生长、抗氧化、小肠形态及空肠紧密连接蛋白基因表达的影响

收稿日期:2022-12-30 接受日期:2023-03-22 出版日期:2024-04-20 发布日期:2024-03-30

Responses of growth performance, antioxidant function, small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

Kaiwen Lei^{1, 2}, Hao Wu^{1, 2}, Jerry W Spears³, Xi Lin³, Xi Wang^{1, 2}, Xue Bai^{1, 2}, Yanling Huang^1,
2#

¹Key Laboratory of Animal Science of State Ethnic Affairs Commission, Southwest Minzu University, Chengdu 610041, China
²Key Laboratory of Qinghai–Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Southwest Minzu University, Chengdu 610041, China
³Department of Animal Science, North Carolina State University, Raleigh, NC 27695-7621, USA

Received:2022-12-30 Accepted:2023-03-22 Online:2024-04-20 Published:2024-03-30
About author:Kaiwen Lei, E-mail: lkw19960813@163.com; #Correspondence Yanling Huang, Tel: +86-28-85522310, E-mail: swunylh@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31501977), the Sichuan Provincial Key R&D Project, China (22ZDYF0194), and the Double World-Class Project of Southwest Minzu University, China (XM2023010).

摘要/Abstract

摘要：

本试验旨在研究饲粮不同铁水平添加对1-21日龄黄羽肉鸡生长性能、抗氧化功能、肠道形态和空肠紧密连接蛋白基因表达的影响，探讨饲粮铁添加水平与以上各指标间的剂量效应。将720只1日龄黄羽肉公雏按照体重随机分成9个组，每组8个重复，每个重复10只鸡。对照组饲喂不额外添加铁的基础饲粮（实测铁含量为79.6 mg kg-1），铁添加组分别饲喂以七水硫酸亚铁（FeSO₄·7H₂O）形式添加20、40、60、80、160、320、640或1280 mg kg-1铁的试验饲粮。试验期21 d。各组试鸡于21日龄屠宰分析血浆、肝脏、十二指肠和空肠黏膜抗氧化指标（T-SOD, GSH-Px, MDA）、十二指肠及空肠肠道形态（绒毛高度、隐窝深度、绒隐比）以及十二指肠及空肠紧密链接蛋白（Claudin-1, Occludin, ZO-1）mRNA表达水平。结果表明：与对照组相比，添加20 mg kg^-1铁提高了黄羽肉鸡的平均日增重（ADG）（P<0.0001），而与添加20 mg kg^-1铁组相比，添加640和1280 mg kg^-1铁降低了黄羽肉鸡的ADG（P<0.0001）和平均日采食量（ADFI）（P<0.0001）。黄羽肉鸡血浆和十二指肠中丙二醛（MDA）浓度随饲粮铁添加量的升高直线上升（P<0.0001），肝脏和空肠MDA浓度呈线性（P<0.05）和二次曲线上升（P<0.05）。与添加20 mg kg^-1铁相比，添加640 mg Fe kg^-1和1280 mg kg^-1铁提高了血浆、肝脏和十二指肠中的MDA浓度。十二指肠和空肠绒毛高度（VH）以及十二指肠绒毛高度/隐窝深度（V/C）随饲粮铁添加量的增加线性降低（P˂0.05）。与添加20 mg kg^-1铁相比，添加640 mg Fe kg^-1和1280 mg kg-1铁降低了十二指肠和空肠中的VH（P<0.05）。随着饲粮铁添加量的增加，空肠中闭合蛋白1（Claudin-1）的mRNA表达水平线性降低（P=0.001），咬合蛋白（Occludin）和闭合小环蛋白1（ZO-1）的mRNA表达水平呈线性（P<0.05）和二次曲线降低（P<0.05）。与添加20 mg kg-1铁相比，饲粮320mg kg^-1铁添加降低了空肠Occludin mRNA表达水平（P<0.05），饲粮1280 mg kg^-1铁添加降低了Claudin-1和 ZO-1 mRNA表达水平（P<0.05）。综上所述，黄羽肉鸡饲粮中添加640 mg kg^-1及以上的铁会导致黄羽肉鸡生长性能下降、抗氧化性能降低、肠道形态受损，饲粮1280 mg kg^-1铁添加同时会降低空肠紧密连接蛋白的基因表达。

Abstract: This study aimed to investigate the dose-effect of iron on growth performance, antioxidant function, intestinal morphology, and mRNA expression of jejunal tight junction protein in 1- to 21-d-old yellow-feathered broilers. A total of 720 1-d-old yellow-feathered male broilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage. The dietary treatments were consisted of a basal diet (contained 79.6 mg Fe kg^–1) supplemented with 0, 20, 40, 60, 80, 160, 320, 640, and 1,280 mg Fe kg^–1 in the form of FeSO₄·7H₂O. Compared with the birds in the control group, birds supplemented with 20 mg Fe kg^–1 had higher average daily gain (ADG) (P<0.0001). Adding 640 and 1,280 mg Fe kg^–1 significantly decreased ADG (P<0.0001) and average daily feed intake (ADFI) (P<0.0001) compared with supplementation of 20 mg Fe kg^–1. Malondialdehyde (MDA) concentration in plasma and duodenum increased linearly (P<0.0001), but MDA concentration in liver and jejunum increased linearly (P<0.05) or quadratically (P<0.05) with increased dietary Fe concentration. The villus height (VH) in duodenum and jejunum, and the ratio of villus height to crypt depth (V/C) in duodenum decreased linearly (P˂0.05) as dietary Fe increased. As dietary Fe increased, the jejunal relative mRNA abundance of claudin-1 decreased linearly (P=0.001), but the jejunal relative mRNA abundance of zona occludens-1 (ZO-1) and occludin decreased linearly (P˂0.05) or quadratically (P˂0.05). Compared with the supplementation of 20 mg Fe kg^–1, the supplementation of 640 mg Fe kg^–1 or higher increased (P˂0.05) MDA concentrations in plasma, duodenum, and jejunum, decreased VH in the duodenum and jejunum, and the addition of 1,280 mg Fe kg^–1reduced (P˂0.05) the jejunal tight junction protein (claudin-1, ZO-1, occludin) mRNA abundance. In summary, 640 mg of supplemental Fe kg^–1 or greater was associated with decreased growth performance, increased oxidative stress, disrupted intestinal morphology, and reduced mRNA expression of jejunal tight junction protein.

Key words: iron , yellow-feathered broiler , antioxidant function , intestinal morphology , tight junction protein

. 饲粮铁水平对黄羽肉鸡生长、抗氧化、小肠形态及空肠紧密连接蛋白基因表达的影响[J]. Journal of Integrative Agriculture, 2024, 23(4): 1329-1337.

Kaiwen Lei, Hao Wu, Jerry W Spears, Xi Lin, Xi Wang, Xue Bai, Yanling Huang.

Responses of growth performance, antioxidant function, small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers [J]. Journal of Integrative Agriculture, 2024, 23(4): 1329-1337.

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饲粮铁水平对黄羽肉鸡生长、抗氧化、小肠形态及空肠紧密连接蛋白基因表达的影响

Responses of growth performance, antioxidant function, small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

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