野生䴙䴘种群中监测到H5N8亚型高致病性禽流感病毒

doi:10.1016/j.jia.2023.09.026

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (1): 348-353.DOI: 10.1016/j.jia.2023.09.026

野生䴙䴘种群中监测到H5N8亚型高致病性禽流感病毒

收稿日期:2023-05-25 接受日期:2023-08-15 出版日期:2024-01-20 发布日期:2024-01-07

Emergence of highly pathogenic avian influenza A (H5N8) clade 2.3.4.4b viruses in grebes in Inner Mongolia and Ningxia, China, in 2021

Qiuzi Xu^1*, Xinru Lü^1*, Yi Li^1*, Hua Luo¹, Zhen Zhang¹, Xiang Li¹, Rongxiu Qin¹, Qing An¹, Fengyi Qu¹, Zhenliang Zhao¹, Chengbo Zhang⁴, Weidong Wang⁵, Yuecheng Li⁵, Yajun Wang¹, Xiangwei Zeng¹, Zhijun Hou¹, Jingqiang Ren^3#, Yulong Wang^1#, Yanbing Li^2#, Hongliang Chai^1#

¹College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China
²Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150040, China
³Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou 325000, China
⁴Ordos Forestry and Grassland Administration, Ordos 017000, China
⁵Monitoring Center for Terrestrial Wildlife Epidemic Diseases, Yinchuan 750000, China

Received:2023-05-25 Accepted:2023-08-15 Online:2024-01-20 Published:2024-01-07
About author:#Correspondence Hongliang Chai, Tel: +86-18745051188, E-mail: Hongliang_Chai@hotmail.com; Yanbing Li, Tel: +86-451-51997168, E-mail: liyanbing@caas.cn; Yulong Wang, Tel: +86-13946025335, E-mail: yulongwang73@hotmail.com; Jingqiang Ren, Tel: +86-15943085036, E-mail: rjq207@163.com * These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31970501), the Fundamental Research Funds for the Central Universities, China (2572022CG01), and the National Forestry and Grassland Administration, China.

摘要/Abstract

摘要：

2021年5月至6月，在中国内蒙古和宁夏地区死亡䴙䴘及其周围环境样品中监测到10株H5N8亚型高致病性禽流感病毒，10株H5N8亚型病毒的序列分析和遗传衍化结果显示，该病毒属于2.3.4.4b分支，碱性裂解位点为REKRRKR↓GLF，符合高致病性禽流感病毒分子特征。在遗传进化上，除分离株E42的NP基因外，其余分离株的所有基因片段高度同源且与2020年秋季在中国流行的H5N8亚型病毒具有相同进化来源；毒株E42的NP基因则与2019-2020年在中亚水禽中流行的低致病性禽流感病毒聚类在一起。分析表明，自2020年秋季以来，H5N8亚型高致病性禽流感病毒在中国持续存在，并在进化过程中与水禽低致病性禽流感病毒发生了重组。

为了更加深入的了解H5N8亚型禽流感病毒的传播动态，我们选取了2020年秋季在中国流行的H5N8亚型高致病性禽流感病毒毒株与本研究H5N8亚型分离株的全基因组进行单倍型遗传网络分析，结果表明，本次H5N8亚型分离株与2020年秋季在湖北、河南三门峡和内蒙古发生的H5N8亚型病毒之间的核苷酸差异性较低，表明病毒可能在它们之间存在传播。

现场调查发现：2021年5月下旬以来，采样区域的黑颈䴙䴘种群开始发病死亡，并且出现扭颈、共济失调等禽流感典型神经症状。结合已有研究数据，可以得出：䴙䴘对H5亚型高致病性禽流感病毒具有较高易感性，可以作为高致病性禽流感病毒的指示性物种，进行长期监测和预警。

. 野生䴙䴘种群中监测到H5N8亚型高致病性禽流感病毒[J]. Journal of Integrative Agriculture, 2024, 23(1): 348-353.

Qiuzi Xu, Xinru Lü, Yi Li, Hua Luo, Zhen Zhang, Xiang Li, Rongxiu Qin, Qing An, Fengyi Qu, Zhenliang Zhao, Chengbo Zhang, Weidong Wang, Yuecheng Li, Yajun Wang, Xiangwei Zeng, Zhijun Hou, Jingqiang Ren, Yulong Wang, Yanbing Li, Hongliang Chai.

Emergence of highly pathogenic avian influenza A (H5N8) clade 2.3.4.4b viruses in grebes in Inner Mongolia and Ningxia, China, in 2021 [J]. Journal of Integrative Agriculture, 2024, 23(1): 348-353.

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野生䴙䴘种群中监测到H5N8亚型高致病性禽流感病毒

Emergence of highly pathogenic avian influenza A (H5N8) clade 2.3.4.4b viruses in grebes in Inner Mongolia and Ningxia, China, in 2021

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