1167份中国大豆种质类胡萝卜素和叶绿素含量的自然变异和地理分布分析

doi:10.1016/j.jia.2022.10.011

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (9): 2632-2647.DOI: 10.1016/j.jia.2022.10.011

1167份中国大豆种质类胡萝卜素和叶绿素含量的自然变异和地理分布分析

收稿日期:2022-05-11 接受日期:2022-09-09 出版日期:2023-09-20 发布日期:2023-09-14

Natural variations and geographical distributions of seed carotenoids and chlorophylls in 1 167 Chinese soybean accessions

Berhane S. GEBREGZIABHER^{1, 2*}, ZHANG Sheng-rui^1*, Muhammad AZAM¹, QI Jie¹, Kwadwo G. AGYENIM-BOATENG¹, FENG Yue¹, LIU Yi-tian¹, LI Jing¹, LI Bin^3#, SUN Jun-ming^1#

¹ National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
² Crop Sciences Research Department, Mehoni Agricultural Research Center, Maichew 7020, Ethiopia
³ MARA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2022-05-11 Accepted:2022-09-09 Online:2023-09-20 Published:2023-09-14
About author:Berhane S. Gebregziabher, E-mail: berhane76@gmail.com; ZHANG Sheng-rui, E-mail: zhangshengrui@caas.cn; #Correspondence SUN Jun-ming, Tel: +86-10-82105805, E-mail: sunjunming@caas.cn; LI Bin, Tel/Fax: +86-10-82105805, E-mail: libin02@caas.cn * These authors contributed equally to this study.
Supported by:
This work was financially supported by the National Natural Science Foundation of China (32161143033 and 32001574) and the Agricultural Science and Technology Innovation Program of CAAS (2060203-2).

摘要/Abstract

摘要：

厘清大豆种质籽粒中的类胡萝卜素组分和含量对大豆种质营养品质评价具有重要意义。本研究针对遗传多样性丰富的中国大豆种质的类胡萝卜素和叶绿素含量进行了系统分析，并揭示了不同营养品质组分间的相关性。结果显示基因型、种植年份、种质类型、子叶色和生态区来源显著影响籽粒中类胡萝卜素和叶绿素含量，其平均类胡萝卜素总含量变化范围为8.15-14.72 µg g^-1。大豆农家种的类胡萝卜素含量是栽培种的1.2倍，特别是绿子叶种质的类胡萝卜素和叶绿素含量显著高于黄子叶种质。需要指出的是单一组分中叶黄素的含量是最高的，其变化范围为1.35-37.44 µg g^-1。类胡萝卜素和叶绿素含量与其他品质组分显著相关，这有利于育种者在强化类胡萝卜素含量同时而不影响其他品质组分。我们结果证明了大豆籽粒中的类胡萝卜素含量是丰富的，但其积累宜受遗传因素、种质类型和种质来源的显著影响。我们还鉴定了一批高类胡萝卜素含量的大豆新种质，可以为大豆育种、食品加工和化妆品行业利用。

Abstract: Understanding the composition and contents of carotenoids in various soybean seed accessions is important for their nutritional assessment. This study investigated the variability in the concentrations of carotenoids and chlorophylls and revealed their associations with other nutritional quality traits in a genetically diverse set of Chinese soybean accessions comprised of cultivars and landraces. Genotype, planting year, accession type, seed cotyledon color, and ecoregion of origin significantly influenced the accumulation of carotenoids and chlorophylls. The mean total carotenoid content was in the range of 8.15–14.72 µg g^–1 across the ecoregions. The total carotenoid content was 1.2-fold higher in the landraces than in the cultivars. Soybeans with green cotyledons had higher contents of carotenoids and chlorophylls than those with yellow cotyledons. Remarkably, lutein was the most abundant carotenoid in all the germplasms, ranging from 1.35–37.44 µg g^–1. Carotenoids and chlorophylls showed significant correlations with other quality traits, which will help to set breeding strategies for enhancing soybean carotenoids without affecting the other components. Collectively, our results demonstrate that carotenoids are adequately accumulated in soybean seeds, however, they are strongly influenced by genetic factors, accession type, and germplasm origin. We identified novel germplasms with the highest total carotenoid contents across the various ecoregions of China that could serve as the genetic materials for soybean carotenoid breeding programs, and thereby as the raw materials for food sectors, pharmaceuticals, and the cosmetic industry.

Key words: accession type , carotenoid , chlorophyll , ecoregion , geographical distribution , Pearson’s correlation , soybean (Glycine max L. Merrill)

Berhane S. GEBREGZIABHER, ZHANG Sheng-rui, Muhammad AZAM, QI Jie, Kwadwo G. AGYENIM-BOATENG, FENG Yue, LIU Yi-tian, LI Jing, LI Bin, SUN Jun-ming. 1167份中国大豆种质类胡萝卜素和叶绿素含量的自然变异和地理分布分析[J]. Journal of Integrative Agriculture, 2023, 22(9): 2632-2647.

Berhane S. GEBREGZIABHER, ZHANG Sheng-rui, Muhammad AZAM, QI Jie, Kwadwo G. AGYENIM-BOATENG, FENG Yue, LIU Yi-tian, LI Jing, LI Bin, SUN Jun-ming. Natural variations and geographical distributions of seed carotenoids and chlorophylls in 1 167 Chinese soybean accessions[J]. Journal of Integrative Agriculture, 2023, 22(9): 2632-2647.

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1167份中国大豆种质类胡萝卜素和叶绿素含量的自然变异和地理分布分析

Natural variations and geographical distributions of seed carotenoids and chlorophylls in 1 167 Chinese soybean accessions

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