转录组和脂质组联合分析揭示棉花纤维中低K+信号缓解缺Ca2+效应的机制

doi:10.1016/j.jia.2023.01.002

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (8): 2306-2322.DOI: 10.1016/j.jia.2023.01.002

转录组和脂质组联合分析揭示棉花纤维中低K⁺信号缓解缺Ca²⁺效应的机制

收稿日期:2022-08-10 接受日期:2022-10-12 出版日期:2023-08-20 发布日期:2022-10-12

Comparative transcriptome and lipidome reveal that a low K⁺ signal effectively alleviates the effect induced by Ca²⁺ deficiency in cotton fibers

GUO Kai^1*, GAO Wei^2*, ZHANG Tao-rui¹, WANG Zu-ying¹, SUN Xiao-ting¹, YANG Peng¹, LONG Lu², LIU Xue-ying¹, WANG Wen-wen¹, TENG Zhong-hua¹, LIU Da-jun¹, LIU De-xin¹, TU Li-li³, ZHANG Zheng-sheng^1#

¹ College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, P.R.China
² State Key Laboratory of Cotton Biology/Henan Key Laboratory of Plant Stress Biology/School of Life Sciences, Henan University,
Kaifeng 475001, P.R.China
³ National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China

Received:2022-08-10 Accepted:2022-10-12 Online:2023-08-20 Published:2022-10-12
About author:#Correspondence ZHANG Zheng-sheng, E-mail: zhangzs@swu.edu.cn * These authors contributed equally to this study.
Supported by:

This project was supported by the National Natural Science Foundation of China (31901577), the Fundamental Research Funds for the Central Universities, China (SWU-KT22035), and the State Key Laboratory of Cotton Biology Open Fund, China (C B2021A32).

摘要/Abstract

摘要：

Ca²⁺离子在维持细胞壁以及细胞膜的完整性中具有重要作用，是植物生长和发育中必不可少的矿质营养元素之一。解析Ca²⁺离子在糖代谢和脂代谢中的作用能够为理解棉花纤维快速伸长阶段细胞膜和细胞壁的动态变化提供有意义的参考。本研究利用胚珠培养系统发现缺Ca²⁺会促进纤维和胚珠细胞的膨大，但同时也会诱导组织的褐化。RNA-seq差异表达基因分析发现缺Ca²⁺使细胞处于一个较高的氧化态，并且激活与糖代谢和脂代谢相关的基因的表达。尤其以糖酵解途径变化最为显著，其代谢途径中的9个酶相关的基因上调表达，缺Ca²⁺处理细胞中的葡萄糖含量显著下降，改变了糖酵解途径的流动。低K⁺能够恢复缺Ca²⁺诱导糖酵解途径相关基因的表达以及葡萄糖的含量。采用电喷雾电离串联质谱技术检测了不同Ca²⁺、K⁺离子处理条件下细胞中脂质组成分的动态变化。缺Ca²⁺处理细胞中自由脂肪酸（FA）、二酰甘油（DAG）和糖脂含量降低，三酰甘油（DAG）磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、磷脂酰胆碱（PC）含量增加。低K⁺与缺Ca²⁺的互作信号能够恢复FA、磷脂、糖脂含量至正常水平，有效缓解缺Ca²⁺效应。本研究通过在转录和代谢水平的比较分析，揭示了Ca²⁺和K⁺信号互作在维持纤维快速伸长过程中糖酵解和脂代谢中发挥着重要作用。

Abstract: Calcium (Ca²⁺) plays an important role in determining plant growth and development because it maintains cell wall and
membrane integrity. Therefore, understanding the role of Ca2+ in carbon and lipid metabolism could provide insights
into the dynamic changes in cell membranes and cell walls during the rapid elongation of cotton fibers. In the present
study, we found that the lack of Ca²⁺ promoted fiber elongation and rapid ovule expansion, but it also caused tissue
browning in the ovule culture system. RNA-sequencing revealed that Ca²⁺ deficiency induced cells to be highly oxidized,
and the expression of genes related to carbon metabolism and lipid metabolism was activated significantly. All gene
members of nine key enzymes involved in glycolysis were up-regulated, and glucose was significantly reduced in Ca²⁺
deficiency-treated tissues. Ca²⁺ deficiency adjusted the flowing of glycolysis metabolic. However, low K⁺ recovered
the expression levels of glycolysis genes and glucose content caused by Ca2+ deficiency. Electrospray ionizationtandem
mass spectrometry technology was applied to uncover the dynamic profile of lipidome under Ca²⁺ and K⁺
interacted conditions. Ca²⁺ deficiency led to the decrease of fatty acid (FA), diacylglycerol (DAG), glycolipid and the
significant increase of triacylglycerol (TAG), phospholipid phosphatidylethanolamine (PE), phosphatidylglycerol (PG),
and PC (phosphatidylcholine). Low K⁺ restored the contents of FA, phospholipids, and glycolipids, effectively relieved
the symptoms caused by Ca²⁺ deficiency, and recovered the development of fiber cells. This study revealed dynamic
changes in transcript and metabolic levels and uncovered the signaling interaction of Ca²⁺ deficiency and low K⁺ in
glycolysis and lipid metabolism during fiber development.

Key words: cotton fiber , glycolysis , lipidome , calcium , potassium

GUO Kai, GAO Wei, ZHANG Tao-rui, WANG Zu-ying, SUN Xiao-ting, YANG Peng, LONG Lu, LIU Xue-ying, WANG Wen-wen, TENG Zhong-hua, LIU Da-jun, LIU De-xin, TU Li-li, ZHANG Zheng-sheng. 转录组和脂质组联合分析揭示棉花纤维中低K⁺信号缓解缺Ca²⁺效应的机制[J]. Journal of Integrative Agriculture, 2023, 22(8): 2306-2322.

GUO Kai, GAO Wei, ZHANG Tao-rui, WANG Zu-ying, SUN Xiao-ting, YANG Peng, LONG Lu, LIU Xue-ying, WANG Wen-wen, TENG Zhong-hua, LIU Da-jun, LIU De-xin, TU Li-li, ZHANG Zheng-sheng. Comparative transcriptome and lipidome reveal that a low K⁺ signal effectively alleviates the effect induced by Ca²⁺ deficiency in cotton fibers[J]. Journal of Integrative Agriculture, 2023, 22(8): 2306-2322.

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转录组和脂质组联合分析揭示棉花纤维中低K⁺信号缓解缺Ca²⁺效应的机制

Comparative transcriptome and lipidome reveal that a low K⁺ signal effectively alleviates the effect induced by Ca²⁺ deficiency in cotton fibers

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