一个调控花生荚果形状的稳定主效数量性状位点

doi:10.1016/j.jia.2023.02.005

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (8): 2323-2334.DOI: 10.1016/j.jia.2023.02.005

一个调控花生荚果形状的稳定主效数量性状位点

收稿日期:2022-08-18 接受日期:2022-12-19 出版日期:2023-08-20 发布日期:2023-08-04

A stable and major QTL region on chromosome 2 conditions pod shape in cultivated peanut (Arachis hyopgaea L.)

ZHANG Sheng-zhong¹, HU Xiao-hui¹, WANG Fei-fei¹, CHU Ye², YANG Wei-qiang¹, XU Sheng³, WANG Song⁴, WU Lan-rong⁵, YU Hao-liang⁶, MIAO Hua-rong^1#, FU Chun^7#, CHEN Jing^1#

¹Shandong Peanut Research Institute, Qingdao 266100, P.R.China
² Department of Horticulture, University of Georgia Tifton Campus, Tifton, GA 31793, the United States
³ Anhui General Station of Agricultural Technology Extension, Hefei 230001, P.R.China
⁴ Anhui Academy of Agricultural Sciences, Hefei 230001, P.R.China
⁵ Qingdao Agricultural Technology Extension Center, Qingdao 266071, P.R.China
⁶Yantai Fenglin Foodstuff Co., Ltd., Yantai 264108, P.R.China
⁷ Weifang Academy of Agricultural Sciences, Weifang 261071, P.R.China

Received:2022-08-18 Accepted:2022-12-19 Online:2023-08-20 Published:2023-08-04
About author:ZHANG Sheng-zhong, E-mail: 593318769@qq.com; #Correspondence MIAO Hua-rong, E-mail: 1949813628@qq.com; FU Chun, E-mail: nkyhssf@163.com; CHEN Jing, Tel: +86-532-87631512, E-mail: mianbaohua2008@126.com
Supported by:
This research was supported by the National Natural Science Foundation of China (32001584), the Natural Science Foundation of Shandong Province, China (ZR202111290099), the Breeding Project from Department Science & Technology of Shandong Province, China (2020LZGC001), the Agricultural Scientific and the Technological Innovation Project of Shandong Academy of Agricultural Sciences, China (CXGC2022A21, CXGC2022A03, and CXGC2023A06 (A39 and A46)), and the Qingdao People’s Livelihood Science and Technology Program, China (20-3-4-26-nsh).

摘要/Abstract

摘要： 荚果形状是影响花生荚果制品市场价值的重要可遗传性状。为探索该性状内在遗传机制，本研究基于图像分析考察了影响荚果性状的6个组分性状，包括荚果长度、荚果宽度、荚果长宽比、荚果圆度、果喙明显度和缢缩明显度。对含有181个家系的重组自交系群体进行了三个不同环境下的表型测定。结果表明，该群体的6个组分性状均表现为连续分布和超亲遗传，且各组分性状之间表现为不同程度相关性，广义遗传率变异范围为0.87-0.95。数量性状位点（QTL）分析共鉴定到26个加性QTL，分别解释3.79%~52.37%的表型变异。在花生第2染色体上发现一个可调控多个组分性状的稳定主效遗传位点。该位点覆盖10.81 Mb物理区间，包含543个潜在基因，生物信息学分析对候选基因进行了预测。本研究还针对6个组分性状鉴定到73对上位性互作（涉及92个遗传位点），分别可解释0.94%-6.45%表型变异。以上研究结果为荚果形状相关基因的图位克隆与分子标记辅助育种提供了重要研究基础。

Abstract: Peanut pod shape is a heritable trait which affects the market acceptance of in-shell peanut products. In order to determine the genetic control of pod shape, six component traits of pod shape (pod length, pod width, pod length/width ratio, pod roundness, beak degree and constriction degree) were measured using an image-based phenotyping method. A recombinant inbred line (RIL) population consisting of 181 lines was phenotyped across three environments. Continuous distributions and transgressive segregations were demonstrated in all measured traits and environments. Significant correlations were found among most component traits with broad-sense heritability ranging from 0.87 to 0.95. Quantitative trait locus (QTL) analysis yielded 26 additive QTLs explaining 3.79 to 52.37% phenotypic variations. A novel, stable and major QTL region conditioning multiple shape features was detected on chromosome 2, which spans a 10.81-Mb genomic region with 543 putative genes. Bioinformatics analysis revealed several candidate genes in this region. In addition, 73 pairs of epistatic interactions involving 92 loci were identified for six component traits explaining 0.94–6.45% phenotypic variations. These results provide new genetic loci to facilitate genomics-assisted breeding of peanut pod shape.

Key words: peanut , pod shape , quantitative trait locus , additive effect , epistasis

ZHANG Sheng-zhong, HU Xiao-hui, WANG Fei-fei, CHU Ye, YANG Wei-qiang, XU Sheng, WANG Song, WU Lan-rong, YU Hao-liang, MIAO Hua-rong, FU Chun, CHEN Jing. 一个调控花生荚果形状的稳定主效数量性状位点 [J]. Journal of Integrative Agriculture, 2023, 22(8): 2323-2334.

ZHANG Sheng-zhong, HU Xiao-hui, WANG Fei-fei, CHU Ye, YANG Wei-qiang, XU Sheng, WANG Song, WU Lan-rong, YU Hao-liang, MIAO Hua-rong, FU Chun, CHEN Jing. A stable and major QTL region on chromosome 2 conditions pod shape in cultivated peanut (Arachis hyopgaea L.)[J]. Journal of Integrative Agriculture, 2023, 22(8): 2323-2334.

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一个调控花生荚果形状的稳定主效数量性状位点

A stable and major QTL region on chromosome 2 conditions pod shape in cultivated peanut (Arachis hyopgaea L.)

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