Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (5): 1291-1307.DOI: 10.1016/j.jia.2022.08.039
收稿日期:
2022-02-10
接受日期:
2022-04-22
出版日期:
2023-05-20
发布日期:
2022-04-22
LI Jiao-jiao*, ZHAO Li*, LÜ Bo-ya, FU Yu, ZHANG Shu-fa, LIU Shu-hui, YANG Qun-hui, WU Jun, LI Jia-chuang#, CHEN Xin-hong#
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China
Received:
2022-02-10
Accepted:
2022-04-22
Online:
2023-05-20
Published:
2022-04-22
About author:
#Correspondence LI Jia-chuang, E-mail: lijiachuang0208@126.com; CHEN Xin-hong, E-mail: cxh2089@126.com
* These authors contributed equally to this study.
Supported by:
This study was supported by the National Natural Science Foundation of China (31771785) and the Key Research and Development Program of Shaanxi, China (2018ZDXM-NY-006).
摘要: 黑麦(Secale cereale L., 2n=2x=14, RR)因具有多种病害抗性和非生物胁迫耐性受性强的特点而被认为是普通小麦改良的重要遗传资源。源自德国栽培黑麦品种Petkus的1RS染色体在小麦的育种进程中起着举足轻重的作用,但由于其所具有的抗病性逐渐变弱,因此亟需发掘新的抗性资源。本研究从普通小麦和墨西哥黑麦的杂交后代中选育了一个新型衍生后代,命名为D27。细胞学观察显示D27的核型组成为2n=42=21II。基因组原位杂交(GISH)结果表明D27中含有一对整臂易位的墨西哥黑麦染色体,该外源染色体在有丝分裂和减数分裂过程中均能稳定遗传。荧光原位杂交(FISH)和醇溶蛋白电泳分析都表明衍生系D27的小麦1DS染色体发生了丢失,取而代之的是墨西哥黑麦的1RS染色体。这一结果同样得到了小麦简单序列重复(SSR)标记、黑麦特异序列扩增区段(SCAR)标记和小麦40K SNP芯片的验证支持,即:通过分子标记和芯片检测,D27中不含有小麦1DS染色体,但是存在黑麦1RS染色体。农艺性状鉴定表明与小麦亲本相比D27的分蘖数增多,条锈病和白粉病抗性增强。面团特性分析表明,1DS染色体被1RS染色体替换后,衍生系D27的面团粘性增加,弹性变低,有利于蛋糕的制作。综上所述,细胞遗传学稳定的小麦-墨西哥黑麦T1DL·1RS易位系可作为小麦抗病和增产育种的优异新种质,同时可用于黑麦1RS染色体遗传多样性的研究。
LI Jiao-jiao, ZHAO Li, LÜ Bo-ya, FU Yu, ZHANG Shu-fa, LIU Shu-hui, YANG Qun-hui, WU Jun, LI Jia-chuang, CHEN Xin-hong. 具有条锈病和白粉病抗性的小麦-墨西哥黑麦T1DL•1RS易位系新种质的创制与鉴定[J]. Journal of Integrative Agriculture, 2023, 22(5): 1291-1307.
LI Jiao-jiao, ZHAO Li, LÜ Bo-ya, FU Yu, ZHANG Shu-fa, LIU Shu-hui, YANG Qun-hui, WU Jun, LI Jia-chuang, CHEN Xin-hong. Development and characterization of a novel common wheat–Mexico Rye T1DL·1RS translocation line with stripe rust and powdery mildew resistance[J]. Journal of Integrative Agriculture, 2023, 22(5): 1291-1307.
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