Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (2): 336-350.DOI: 10.1016/S2095-3119(20)63594-8

所属专题： 油料作物合辑Oil Crops

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田间霉变诱发大豆籽粒中贮藏的物质分解并导致大豆品质劣变

  


  • 收稿日期:2020-08-14 接受日期:2020-12-17 出版日期:2022-01-02 发布日期:2022-01-02

Field mold stress induced catabolism of storage reserves in soybean seed and the resulting deterioration of seed quality in the field

DENG Jun-cai1, 2, LI Xiao-man1, 2, 3, XIAO Xin-li1, 2, WU Hai-jun1, 2, YANG Cai-qiong1, 2, 3, LONG Xi-yang1, 2, ZHANG Qi-hui1, 2, Nasir Iqbal1, 2, 5, WANG Xiao-chun1, 2, YONG Tai-wen1, 2, DU Jun-bo1, 2, 3, YANG Feng1, 2, LIU Wei-guo1, 2, 3, ZHANG Jing1, 4, WU Xiao-ling1, 2, WU Yu-shan1, 2, YANG Wen-yu1, 2, LIU Jiang1, 2, 3   

  1. 1 Sichuan Engineering Research Center for Crop Strip Intercropping System/Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture and Rural Affairs, Chengdu 611130, P.R.China
    2 College of Agriculture, Sichuan Agricultural University, Chengdu 611130, P.R.China
    3 Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, P.R.China
    4 College of Horticulture, Sichuan Agricultural University, Chengdu 611130, P.R.China
    5 School of Agriculture, Food and Wine, The University of Adelaide, Urrbrae, SA 5064, Australia
  • Received:2020-08-14 Accepted:2020-12-17 Online:2022-01-02 Published:2022-01-02
  • About author:Correspondence YANG Wen-yu, E-mail: mssiyangwy@sicau.edu.cn; LIU Jiang, E-mail: jiangliu@sicau.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (31971853), the National Key Research and Development Program of China (2016YFD0300209) and the Science Fund for Distinguished Young Scholars of Sichuan, China (20JCQN0155).

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摘要：

连阴雨天气导致田间湿度增大，诱发田间霉菌的生长繁殖，并侵染农作物导致田间霉变的发生。在大豆生长后期，因连阴雨天气导致的田间霉变严重影响大豆的产量和品质。为探究田间霉变诱导大豆品质劣变的机制，本研究利用人工降雨室模拟连阴雨天气，诱发大豆籽粒田间霉变，结合转录组学和多种代谢检测平台，解析田间霉变胁迫下大豆品质劣变的生化机理。研究结果表明，田间霉变影响大豆的外观品质，霉变大豆籽粒皱缩、变形，并出现霉斑。田间霉变使大豆籽粒中蛋白质、多糖等储藏性物质的含量降低，导致籽粒百粒重显著下降。转录组分析发现，田间霉变使大豆籽粒中氨基酸代谢、糖酵解、三羧酸循环、脂肪酸β氧化等初生代谢过程加强。代谢组分析结果也表明，霉变大豆籽粒中多种氨基酸、糖类物质、有机酸的含量显著增加，而脂肪酸的含量则显著下降。与此同时，大豆异黄酮作为一类重要的抗逆活性物质，其生物合成在转录水平和代谢水平均受到田间霉变的诱导。田间霉变诱发大豆籽粒的防御机制，通过分解和消耗储藏性物质为防御体系的构建提供能量和底物，但储藏性物质的消耗导致了大豆品质劣变。本研究为深入了解大豆籽粒田间霉变的机制提供了重要的理论基础，同时也为抗田间霉变大豆品种的筛选指明方向。

Abstract: Excessive rainfall provides a favorable condition for field mold infection of plants, which triggers field mold (FM) stress.  If FM stress occurs during the late maturation stage of soybean seed, it negatively affects seed yield and quality.  To investigate the responses of soybean seed against FM stress and identify the underlying biochemical pathways involved, a greenhouse was equipped with an artificial rain producing system to allow the induction of mold growth on soybean seed.  The induced quality changes and stress responses were revealed on the levels of both transcriptome and metabolome.  The results showed that soybean seeds produced under FM stress conditions had an abnormal and inferior appearance, and also contained less storage reserves, such as protein and polysaccharide.  Transcriptional analysis demonstrated that genes involved in amino acid metabolism, glycolysis, tricarboxylic acid, β-oxidation of fatty acids, and isoflavone biosynthesis were induced by FM stress.  These results were supported by a multiple metabolic analysis which exhibited increases in the concentrations of a variety of amino acids, sugars, organic acids, and isoflavones, as well as reductions of several fatty acids.  Reprogramming of these metabolic pathways mobilized and consumed stored protein, sugar and fatty acid reserves in the soybean seed in order to meet the energy and substrate demand on the defense system, but led to deterioration of seed quality.  In general, FM stress induced catabolism of storage reserves and diminished the quality of soybean seed in the field.  This study provides a more profound insight into seed deterioration caused by FM stress.

Key words: seed deterioration , seed quality , primary metabolism , storage reserves , resistance