Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (9): 2382-2394.DOI: 10.1016/S2095-3119(20)63383-4

所属专题： 油料作物合辑Oil Crops

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  • 收稿日期:2020-04-20 出版日期:2021-09-01 发布日期:2021-07-20

Interactive effect of shade and PEG-induced osmotic stress on physiological responses of soybean seedlings

Muhammad Ahsan ASGHAR1, 3, JIANG Heng-ke1, 3, SHUI Zhao-wei1, 2, CAO Xi-yu1, HUANG Xi-yu1, Shakeel IMRAN4, Bushra AHMAD5, ZHANG Hao1, YANG Yue-ning1, SHANG Jing1, 2, YANG Hui1, 2, YU Liang1, 3, LIU Chun-yan1, 3, YANG Wen-yu1, 3, SUN Xin1, 2, 3, DU Jun-bo1, 2, 3 
  

  1. 1 College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R.China
    2 Research Center for Modern Agriculture of the Middle East, Sichuan Agricultural University, Chengdu 611130, P.R.China
    3 Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Sichuan Agricultural University, Chengdu 611130, P.R.China
    4 Department of Agronomy, University of Agriculture, Faisalabad (UAF)|UAF Sub-Campus, Burewala 61010, Pakistan
    5 Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, Pakistan
  • Received:2020-04-20 Online:2021-09-01 Published:2021-07-20
  • Contact: Correspondence SUN Xin, E-mail: sunxin@sicau.edu.cn; DU Jun-bo, E-mail: junbodu@sicau.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (31871552 and 31671445), the Sichuan Science and Technology Program, China (2018HH0108) and the Sichuan Innovation Team Project of National Modern Agricultural Industry Technology System, China (sccxtd-2020-20).

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摘要：

本文探讨了PEG诱导的渗透胁迫与荫蔽效应之间的关系。荫蔽和非荫蔽条件下，大豆品种C-103受到聚乙二醇（PEG-6000）诱导的渗透胁迫。在两种光环境中，PEG诱导的渗透胁迫都显著降低了相对含水量、形态参数、碳水化合物和叶绿素含量，大豆幼苗的渗透调节物质、活性氧和抗氧化酶明显增加。本研究表明，在非荫蔽条件下生长的大豆幼苗，PEG诱导的渗透胁迫比荫蔽下的幼苗产生更多的丙二醛和过氧化氢。同样，在干旱胁迫下，被遮荫的植株比不被荫蔽的植株积累了更多的糖和脯氨酸。因此，本研究结果揭示了未遮荫的植物比遮荫的植物对 PEG诱导的渗透胁迫更敏感，这提示荫蔽可以增强植物对渗透胁迫的保护机制，或者至少不会增强 PEG诱导的渗透胁迫对大豆幼苗的不利影响。


Abstract:

Intensively farmed crops used to experience numerous environmental stresses.  Among these, shade and drought significantly influence the morpho-physiological and biochemical attributes of plants.  However, the interactive effect of shade and drought  on the growth and development of soybean under dense cropping systems has not been reported yet.  This study investigated the interactive effect of PEG-induced osmotic stress and shade on soybean seedlings.  The soybean cultivar viz., C-103 was subjected to PEG-induced osmotic stress from polyethylene glycol 6000 (PEG-6000) under shading and non-shading conditions.  PEG-induced osmotic stress significantly reduced the relative water contents, morphological parameters, carbohydrates and chlorophyll contents under both light environments.  A significant increase was observed in osmoprotectants, reactive oxygen species and antioxidant enzymes in soybean seedlings.  Henceforth, the findings revealed that, seedlings grown under non-shading conditions produced more malondialdehyde and hydrogen peroxide contents as compared to the shade-treated plants when subjected to PEG-induced osmotic stress.  Likewise, the shaded plants accumulated more sugars and proline than non-shaded ones under drought stress.  Moreover, it was found that non-shaded grown plants were more sensitive to PEG-induced osmotic stress than those exposed to shading conditions, which suggested that shade could boost the protective mechanisms against osmotic stress or at least would not exaggerate the adverse effects of PEG-induced osmotic stress in soybean seedlings.    

Key words: shade ,  PEG-induced osmotic stress ,  reactive oxygen species ,  antioxidant enzymes ,  soybean