Improving grain appearance of erect-panicle japonica rice cultivars by introgression of the null gs9 allele

doi:10.1016/S2095-3119(21)63659-6

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (8): 2032-2042.DOI: 10.1016/S2095-3119(21)63659-6

收稿日期:2020-12-23 出版日期:2021-08-01 发布日期:2021-07-20

Improving grain appearance of erect-panicle japonica rice cultivars by introgression of the null gs9 allele

ZHAO Dong-sheng^{1, 2*}, LIU Jin-yu^1*, DING Ai-qiu^1*, ZHANG Tao¹, REN Xin-yu¹, ZHANG Lin¹, LI Qian-feng^{1, 2}, FAN Xiao-lei^{1, 2}, ZHANG Chang-quan^{1, 2}, LIU Qiao-quan^{1, 2}

¹ Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, P.R.China
² Co-innovation Center for Modern Production Technology of Grain Crops, Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China

Received:2020-12-23 Online:2021-08-01 Published:2021-07-20
Contact: Correspondence LIU Qiao-quan, E-mail: qqliu@yzu.edu.cn
About author:* These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31971914), the National Key Research and Development Program of China (2016YFD0100501), the Key Research and Development Program of Jiangsu Province, China (BE2018357), the Science Fund for Distinguished Young Scholars of Jiangsu Province, China (BK20200045), the Jiangsu Agricultural Science and Technology Innovation Fund (CX(18)1001), the Jiangsu PAPD Talent Project, and the Yong Elite Scientists Sponsorship Program by China Association for Science and Technology (2018QNRC001).

摘要/Abstract

摘要：

水稻穗型和籽粒大小不仅对产量形成有显著效应，对稻米品质尤其是外观品质也有重要影响。直立穗性状一般由qpe9-1/dep1等位基因控制，已广泛应用于高产粳稻育种，但其稻米的外观品质往往不够理想。GS9是水稻粒形的重要调控因子，该基因突变后可使稻谷适当变细长，从而改良稻米的外观品质。然而，GS9和qPE9-1/DEP1基因都位于第9染色体，两位点紧密连锁，且两者间的互作关系还不明确，这就制约了它们在现代水稻育种中的应用。蛋白和mRNA表达水平的比较分析显示GS9和qPE9-1独立发挥功能。以含有GS9和qpe9-1等位基因的高产粳稻品种2661(GS9/qpe9-1)为背景，创建了3个近等基因系（NIL），分别携带不同等位基因组合，包括NIL(gs9/qpe9-1)、NIL(GS9/qPE9-1)和NIL(gs9/qPE9-1)。结果显示，GS9和qPE9-1对籽粒大小的调控具有加性效应，在含有qpe9-1等位基因的直立穗粳稻品种中导入功能缺失型gs9等位基因，可在不影响株型和穗型的前提下，降低籽粒垩白，改良籽粒外观。此外，在另一推广的高产粳稻品种武育粳27（WYJ27）背景中，导入gs9等位基因，也表现出相同的效应，进一步证实利用gs9等位基因改良高产粳稻品种籽粒外观的可行性。本研究为直立穗粳稻及相关品种稻米外观品质改良提供了有效策略。

Abstract: The panicle architecture and grain size of rice affect not only grain yield but also grain quality, especially grain appearance. The erect-panicle (EP) trait controlled by the qpe9-1/dep1 allele has been widely used in high-yielding japonica rice breeding, but usually accompanied with moderate appearance of milled rice. The null gs9 allele shows a good potential for improving grain shape and appearance. However, GS9 and qPE9-1/DEP1 loci are tightly linked, and their interaction is unclear, which obviously restricts their utilization in modern rice breeding. In the present study, comparative analyses of protein and mRNA levels revealed that GS9 and qPE9-1 function independently. Three near-isogenic lines (NILs) carrying various allelic combinations of these two loci, NIL (gs9/qpe9-1), NIL (GS9/qPE9-1) and NIL (gs9/qPE9-1), in the EP japonica cultivar 2661 (GS9/qpe9-1) background were developed for genetic interaction analysis. GS9 and qPE9-1 had additive effects on determining grain size, and the null gs9 allele could decrease grain chalkiness and improve grain appearance without affecting plant and panicle architecture in EP japonica cultivars. Additionally, introgression lines (ILs) developed in another released EP japonica cultivar Wuyujing 27 (WYJ27) background showed the same additive effect and the feasibility of utilizing the gs9 allele to improve grain appearance quality in high-yielding EP cultivars. This study provides an effective strategy for rice breeders to improve rice grain appearance in EP japonica and related cultivars.

Key words: rice , GS9 , qPE9-1/DEP1 , genetic interaction , erect panicle , grain appearance

. [J]. Journal of Integrative Agriculture, 2021, 20(8): 2032-2042.

ZHAO Dong-sheng, LIU Jin-yu, DING Ai-qiu, ZHANG Tao, REN Xin-yu, ZHANG Lin, LI Qian-feng, FAN Xiao-lei, ZHANG Chang-quan, LIU Qiao-quan. Improving grain appearance of erect-panicle japonica rice cultivars by introgression of the null gs9 allele[J]. Journal of Integrative Agriculture, 2021, 20(8): 2032-2042.

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Improving grain appearance of erect-panicle japonica rice cultivars by introgression of the null gs9 allele

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