Overexpression of the MADS-box gene SlMBP21 alters leaf morphology and affects reproductive development in tomato

doi:10.1016/S2095-3119(21)63638-9

摘要/Abstract

摘要：

果实产量是番茄最重要的园艺特性。根据前人的报道，SEPMBATA型MADS-box基因SlMBP21具有控制番茄花梗离区形成以及调控萼片大小的功能。然而，我们构建过表达的SlMBP21转基因番茄植株，发现这些转基因植株显示出卷曲的叶子，扭曲和开放的雄蕊，产量降低，并产生了小而轻的种子。我们对功能获得型表型和基因表达水平的研究表明SlMBP21基因在调控叶片形态、花和花序结构、种子大小以及果实产量方面起着重要作用。番茄中SlMBP21的过表达导致叶片卷曲，叶片少，这是由于其对关键的叶片极性相关基因的调控导致上、下轴细胞生长不平衡所致。花和花序结构的缺陷导致果实减少。此外，我们发现了SlMBP21通过抑制参与确定番茄种子发育相关基因的表达并与其他一些MADS-box蛋白(S1AGL11，TAGL1和SlMBP3)相互作用来控制种子大小。因此，这些结果表明SlMBP21的过表达对植物生长和发育，特别是对番茄的果实产量造成多重损害。

Abstract: Fruit yield is the most important horticultural trait of tomato. SlMBP21, a SEPALLATA subclass MADS-box gene has been reported to have functions in regulating pedicel abscission zone identity and development and controlling sepal size in tomato. However, we generated transgenic tomato plants which overexpress SlMBP21 and found the transformants displayed curly leaves, abnormally shaped flowers with twisted and opened stamens, reduced yield parameters, and small and light seeds. Our studies on the gain-of-function phenotype and gene expression level showed that its novel aspects played important roles in determining leaf morphology, flower and inflorescence architecture, and seed size, as well as the fruit yield. Overexpression of SlMBP21 in tomato resulted in curly leaves with fewer leaflets due to the regulation of the critical leaf polarity genes that cause an imbalance between the midvein adaxial–abaxial cell growth. Defects in the architecture of flowers and inflorescences resulted in reduced fruit set. Furthermore, we demonstrated that SlMBP21 plays its role through inhibiting the expression of the genes involved in the determination of seed development in tomato and SlMBP21 protein can interact with other MADS-box protein (SlAGL11, TAGL1 and SlMBP3) to control seed size. Thus, these results suggest that overexpression of SlMBP21 causes multiple types of damage to plant growth and development, especially fruit yield, in tomato.

Key words: overexpression of SlMBP21 , curly leaf , fruit yield , seed size

. [J]. Journal of Integrative Agriculture, 2021, 20(12): 3170-3185.

WANG Yun-shu, GUO Peng-yu, ZHANG Jian-ling, XIE Qiao-li, SHEN Hui, HU Zong-li, CHEN Guo-ping. Overexpression of the MADS-box gene SlMBP21 alters leaf morphology and affects reproductive development in tomato[J]. Journal of Integrative Agriculture, 2021, 20(12): 3170-3185.

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