Evaluation of drought tolerance in ZmVPP1-overexpressing transgenic inbred maize lines and their hybrids

doi:10.1016/S2095-3119(19)62828-5

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (9): 2177-2187.DOI: 10.1016/S2095-3119(19)62828-5

所属专题：玉米遗传育种合辑Maize Genetics · Breeding · Germplasm Resources

收稿日期:2019-04-16 出版日期:2020-09-01 发布日期:2020-08-04

Evaluation of drought tolerance in ZmVPP1-overexpressing transgenic inbred maize lines and their hybrids

JIA Teng-jiao^1*, LI Jing-jing^1*, WANG Li-feng¹, CAO Yan-yong1, MA Juan¹, WANG Hao¹, ZHANG Deng-feng², LI Hui-yong¹

1 Institute of Cereal Crop, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China
2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100089, P.R.China

Received:2019-04-16 Online:2020-09-01 Published:2020-08-04
Contact: Correspondence LI Hui-yong, Tel: +86-371-65758930, E-mail: lihuiyong1977@
About author:JIA Teng-jiao, E-mail: jiatengjiao1983@126.com; 126.com; ZHANG Deng-feng, Tel: +86-10-62186652, E-mail: zhangdengfeng@caas.cn * These authors contributed equally to this study.
Supported by:
This study was supported by the National Key Project for Research on Transgenic Plants, China (2016ZX08003-004) and the Independent Innovation Project of Henan Academy of Agricultural Sciences, China (2060302).

摘要/Abstract

Abstract:

The vacuolar proton-pumping pyrophosphatase gene (VPP) is often used to enhance plant drought tolerance via genetic engineering. In this study, the drought tolerance of four transgenic inbred maize lines overexpressing ZmVPP1 (PH4CV-T, PH6WC-T, Chang7-2-T, and Zheng58-T) and their transgenic hybrids was evaluated at various stages. Under normal and drought conditions, the height and fresh weight were greater for the four transgenic inbred maize lines than for the wild-type (WT) controls at the germination and seedling stages. Additionally, the transgenic plants exhibited enhanced photosynthetic efficiency at the seedling stage. In irrigated and non-irrigated fields, the four transgenic lines grew normally, but with increased ear weight and yield compared with the WT plants. Moreover, the ear weight and yield of the transgenic hybrids resulting from the PH4CV-T×PH6WC-W and Chang7-2-T×Zheng58-W crosses increased in the non-irrigated field. Our results demonstrated that the growth and drought tolerance of four transgenic inbred maize lines with improved photosynthesis were enhanced by the overexpression of ZmVPP1. Moreover, the Chang7-2 and PH4CV transgenic lines may be useful for future genetic improvements of maize hybrids to increase drought tolerance.

Key words: inbred maize lines , drought stress , ZmVPP1 , photosynthesis

. [J]. Journal of Integrative Agriculture, 2020, 19(9): 2177-2187.

JIA Teng-jiao, LI Jing-jing, WANG Li-feng, CAO Yan-yong, MA Juan, WANG Hao, ZHANG Deng-feng, LI Hui-yong. Evaluation of drought tolerance in ZmVPP1-overexpressing transgenic inbred maize lines and their hybrids[J]. Journal of Integrative Agriculture, 2020, 19(9): 2177-2187.

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Evaluation of drought tolerance in ZmVPP1-overexpressing transgenic inbred maize lines and their hybrids

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