The circulation of unique reassortment strains of infectious bursal disease virus in Pakistan

doi:10.1016/S2095-3119(20)63183-5

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (7): 1867-1875.DOI: 10.1016/S2095-3119(20)63183-5

收稿日期:2019-09-18 出版日期:2020-07-01 发布日期:2020-05-24

The circulation of unique reassortment strains of infectious bursal disease virus in Pakistan

Altaf HUSSAIN^{1, 2*}, WU Tian-tian^{1, 2*}, FAN Lin-jin^{1, 2}, WANG Yu-long^{1, 2}, Farooq Khalid MUHAMMAD⁴, JIANG Nan^{1, 2}, GAO Li^{1, 2}, LI Kai^{1, 2}, GAO Yu-long^{1, 2}, LIU Chang-jun¹, CUI Hong-yu^{1, 2}, PAN Qing¹, ZHANG Yan-ping¹, Asim ASLAM⁵, Khan MUTI-UR-REHMAN⁵, Muhammad Imran ARSHAD⁶, Hafiz Muhammad ABDULLAH⁷, WANG Xiao-mei^{1, 2, 3}, QI Xiao-le^{1, 2}

¹ Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China
² OIE Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China
³ Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, P.R.China
⁴ Sub-campus Toba Tek Singh, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan
⁵ Pathology Department, University of Veterinary and Animal Sciences, Lahore 54660, Pakistan
⁶ Institute of Microbiology, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan
⁷ Department of Animal Sciences, Ohio State University, Columbus 43210, USA

Received:2019-09-18 Online:2020-07-01 Published:2020-05-24
Contact: Correspondence QI Xiao-le, E-mail: qixiaole@caas.cn; WANG Xiao-mei, Tel: +86-451-51051606, Fax: +86-451-51997166, E-mail: wangxiaomei@caas.cn
About author:Altaf HUSSAIN, E-mail: altaf_virk@yahoo.com; WU Tian-tian, E-mail: ttw19941101@163.com; * These authors contributed equally to this study.
Supported by:
This work was supported by the National Key Research and Development Program of China (2016YFE0203200, 2017YFD0500704), the Heilongjiang Province Foundation for the National Key Research and Development Program of China (GX18B011), the National Natural Science Foundation of China (31430087), and the earmarked fund for the China Agriculture Research System (CARS-41-G15).

摘要/Abstract

摘要： 传染性法氏囊病（Infectious bursal disease，IBD）是由传染性法氏囊病病毒（Infectious bursal disease virus，IBDV）引起的。IBD是危害养禽业的最重要的免疫抑制病之一，是影响全球养禽业可持续发展的重要限制因素。IBD在巴基斯坦也是危害养禽业的重要疫病，然而该国IBDV优势流行毒株尚不明确。本研究针对巴基斯坦主要养禽地区（旁遮普省、信德省、俾路支省和首都伊斯兰堡），开展了IBDV的流行病学研究。之前报道的巴基斯坦IBDV毒株仅显示了A节段编码的VP2基因，在GenBank上未见到过其B节段编码的VP1基因。然而，IBDV的基因组分为A、B两个节段，单独的VP2基因是无法科学地反映IBDV的真实特征的。在本研究中，运用RT-PCR技术，对覆盖29个肉鸡场的36株IBDV进行了VP1和VP2基因代表区段的扩增、测序和分析。基因遗传进化树和同源率比较研究结果显示，本研究的全部36株巴基斯坦IBDV毒株的VP2基因属于IBDV超强毒（very virulent IBDV，IBDV），而VP1基因则独立于超强毒和非超强毒之外形成了独特分支，这类毒株属于独特型节段重组IBDV毒株（vv-A/Uniq-B）。与流行于中国、印度等国家的vv-A/Uniq-B型IBDV相比，巴基斯坦IBDV毒株形成一个独立的亚群，具有明显的地域特点。本研究首次证明，具有地域特点的独特的节段重组毒株（vv-A/Uniq-B）是巴基斯坦IBDV的重要流行毒株。本研究揭示了巴基斯坦IBDV毒株的分子特征，这对于IBD疫苗的科学选择和该病的有效防控具有重要意义。

Abstract:

Infectious bursal disease (IBD), caused by IBD virus (IBDV), is one of the most devastating and immunosuppressive diseases of the poultry and has been a constraint on the sustainable poultry production around the globe including Pakistan. While the disease is threatening the poultry industry, the nature of predominant strains of IBDV in Pakistan remained ill-defined. In this study, an epidemiology survey was conducted in the main chicken-farming regions of Pakistan. The batch of Pakistan IBDVs genes simultaneously covering both VP1 and VP2 were amplified, sequenced, and analyzed. The unique segment-reassortant IBDVs (vv-A/Uniq-B), carrying segment A from vvIBDV and segment B from one unique ancestor, were identified as one important type of circulating strains in Pakistan. The data also discovered the characteristic molecular features of Pakistan IBDVs, which will contribute to scientific vaccine selection and effective prevention of the disease.

Key words: infectious bursal disease virus , circulating strains , reassortant , Pakistan

. [J]. Journal of Integrative Agriculture, 2020, 19(7): 1867-1875.

Altaf HUSSAIN, WU Tian-tian, FAN Lin-jin, WANG Yu-long, Farooq Khalid MUHAMMAD, JIANG Nan, GAO Li, LI Kai, GAO Yu-long, LIU Chang-jun, CUI Hong-yu, PAN Qing, ZHANG Yan-ping, Asim ASLAM, Khan MUTI-UR-REHMAN, Muhammad Imran ARSHAD, Hafiz Muhammad ABDULLAH, WANG Xiao-mei, QI Xiao-le. The circulation of unique reassortment strains of infectious bursal disease virus in Pakistan[J]. Journal of Integrative Agriculture, 2020, 19(7): 1867-1875.

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The circulation of unique reassortment strains of infectious bursal disease virus in Pakistan

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