FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum

doi:10.1016/S2095-3119(19)62819-4

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (7): 1813-1824.DOI: 10.1016/S2095-3119(19)62819-4

所属专题：植物病理合辑Plant Protection—Plant Pathology；植物细菌真菌合辑Plant Bacteria/Fungus

收稿日期:2019-06-14 出版日期:2020-07-01 发布日期:2020-05-24

FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum

LÜ Wu-yun, YANG Nan, XU Zhe, DAI Han, TANG Shuai, WANG Zheng-yi

State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R.China

Received:2019-06-14 Online:2020-07-01 Published:2020-05-24
Contact: Correspondence WANG Zheng-yi, Mobile: +86-18067908283, E-mail: zhywang@zju.edu.cn
About author: Lü Wu-yun, Mobile: +86-15336512118, E-mail: Lvwuyun_blue@163.com;
Supported by:
This work was supported by the National Key Basic Research and Development Program of China (2013CB127802).

摘要/Abstract

Abstract:

Histone lysine acetylation is catalyzed by acetyltransferases (HATs), which is important in regulating gene expression and physiological function in eukaryotic cells. HATs can be classified into two main types: A- and B-type HATs. Recently, in Fusarium graminearum, it has been reported that A-type HATs are involved in hyphal development, conidiation, sexual reproduction and virulence. However, the biological roles of B-type HATs are unknown. Here we report the identification and characterization of two B-type HATs (FgHat1 and FgHat2) in F. graminearum. Targeted deletion of FgHAT1 did not result in any detectable phenotypes. However, ΔFghat2 mutants were severely defective in vegetative growth, conidia production and morphogenesis, deoxynivalenol (DON) biosynthesis and virulence. Interestingly, deletion of FgHAT2 resulted in significantly increased sensitivity to the DNA-damaging agent methyl methanesulfonate (MMS). Furthermore, double deletion mutants (ΔFghat1ΔFghat2) displayed similar phenotypes to the ΔFghat2 mutants. Taken together, we conclude that FgHat2 but not FgHat1 plays essential roles in regulating morphogenesis, DNA damage repair, DON production and virulence in F. graminearum.

Key words: Fusarium graminearum , acetyltransferases , FgHAT2 , pathogenicity

. [J]. Journal of Integrative Agriculture, 2020, 19(7): 1813-1824.

Lü Wu-yun, YANG Nan, XU Zhe, DAI Han, TANG Shuai, WANG Zheng-yi. FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum[J]. Journal of Integrative Agriculture, 2020, 19(7): 1813-1824.

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FgHAT2 is involved in regulating vegetative growth, conidiation, DNA damage repair, DON production and virulence in Fusarium graminearum

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