Effect of transferring lignocellulose-degrading bacteria from termite to rumen fluid of sheep on in vitro gas production, fermentation parameters, microbial populations and enzyme activity

doi:10.1016/S2095-3119(19)62854-6

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (5): 1323-1331.DOI: 10.1016/S2095-3119(19)62854-6

所属专题：动物营养合辑Animal Nutrition

收稿日期:2019-03-19 出版日期:2020-04-01 发布日期:2020-03-25

Effect of transferring lignocellulose-degrading bacteria from termite to rumen fluid of sheep on in vitro gas production, fermentation parameters, microbial populations and enzyme activity

Ayoub AZIZI¹, Afrooz SHARIFI², Hasan FAZAELI³, Arash AZARFAR¹, Arjan JONKER⁴, Ali KIANI¹

¹ Department of Animal Science, Faculty of Agriculture, Lorestan University, Khorramabad 6815144316, Iran
² Animal Science Research Department, Khuzestan Agricultural and Natural Resources Research and Education Center, Ahvaz 613353341, Iran
³ Animal Science Research Institute, Agriculture, Education and Extension Organization, Karaj 315, Iran
⁴ Grasslands Research Centre, AgResearch Ltd., Palmerston North 4442, New Zealand

Received:2019-03-19 Online:2020-04-01 Published:2020-03-25
Contact: Correspondence Ayoub Azizi, E-mail: azizi.ay@lu.ac.ir, azizi.msc.modarese@gmail.com
Supported by:
The authors thank Lorestan University, Iran, for its financial support.

摘要/Abstract

Abstract:

The digestive tract of termite (Microcerotermes diversus) contains a variety of lignocellulose-degrading bacteria with exocellulases enzyme activity, not found in the rumen, which could potentially improve fiber degradation in the rumen. The objectives of the current study were to determine the effect of inoculation of rumen fluid (RF) with three species of bacteria isolated from termite digestive tract, Bacillus licheniformis, Ochrobactrum intermedium, and Microbacterium paludicola, on in vitro gas production (IVGP), fermentation parameters, nutrient disappearance, microbial populations, and hydrolytic enzyme activities with fibrous wheat straw (WS) and date leaf (DL) as incubation substrate. Inoculation of RF with either of three termite bacteria increased (P<0.05) ammonia-N concentration compared with the control group (free of termite gut bacteria). Termite bacteria inoculation had no effect (P>0.05) on gas production characteristics, dry matter, organic matter and neutral detergent fiber disappearance, pH, and concentration and composition of volatile fatty acids. Population of proteolytic bacteria and protozoa, but not cellulolytic bacteria, were increased (P<0.05) when RF was inoculated with termite bacteria with both WS and DL substrates. Inoculation of RF with termite bacteria increased protease activity, while activities of carboxymethyl-cellulase, microcrystalline-cellulase, α-amylase and filter paper degrading activity remained unchanged (P>0.05). Overall, the results of this study indicated that transferring lignocellulose-degrading bacteria, isolated from digestive tract of termite, to rumen liquid increased protozoa and proteolytic bacteria population and consequently increased protease activity and ammonia-N concentration in vitro, however, no effect on fermentation and fiber degradation parameters were detected. These results suggest that the termite bacteria might be rapidly lysed by the rumen microbes before beneficial effects on the rumen fermentation process could occur.

Key words: enzyme activity , gas production , lignocellulose-degrading bacteria , microbial population , termite bacteria

. [J]. Journal of Integrative Agriculture, 2020, 19(5): 1323-1331.

Ayoub AZIZI, Afrooz SHARIFI, Hasan FAZAELI, Arash AZARFAR, Arjan JONKER, Ali KIANI.

Effect of transferring lignocellulose-degrading bacteria from termite to rumen fluid of sheep on in vitro gas production, fermentation parameters, microbial populations and enzyme activity

[J]. Journal of Integrative Agriculture, 2020, 19(5): 1323-1331.

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Effect of transferring lignocellulose-degrading bacteria from termite to rumen fluid of sheep on in vitro gas production, fermentation parameters, microbial populations and enzyme activity

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