The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage

doi:10.1016/S2095-3119(19)62707-3

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (3): 838-847.DOI: 10.1016/S2095-3119(19)62707-3

收稿日期:2018-11-27 出版日期:2020-03-01 发布日期:2020-03-04

The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage

GUO Gang^1*, SHEN Chen^1*, LIU Qiang¹ , ZHANG Shuan-lin¹ , SHAO Tao² , WANG Cong¹ , WANG Yongxin¹ , XU Qing-fang¹, HUO Wen-jie¹

¹ College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.China

² Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, P.R.China

Received:2018-11-27 Online:2020-03-01 Published:2020-03-04
Contact: Correspondence HUO Wen-jie, Tel: +86-354-6287335, Fax: +86-354-6288052, E-mail: huohuo1982@163.com
About author:GUO Gang, E-mail: gggg1984@163.com; SHEN Chen, E-mail: shenchen63029@163.com; * These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31502015, 31672488) and the Natural Science Foundation of Shanxi Province of China (2015021162).

摘要/Abstract

Abstract: The objective of this study was to investigate the effect of lactic acid bacteria (LAB) inoculums on fermentation quality and in vitro digestibility of corn stover silage. Corn stover was ensiled without (control) or with Lactobacillus plantarum (LP), Enterococcus faecalis (EF), and Enterococcus mundtii (EM) for 45 days. The fermentation characteristics were assessed, and subsequent in vitro dry matter digestibility (DM-D), neutral detergent fiber digestibility (NDF-D), volatile fatty acids (VFA), methane (CH₄) production, cellulolytic bacteria proportions and their activities per corn stover silage were also determined. There was no significant difference (P>0.05) among the silage pH, lactic acid, crude protein (CP), water soluble carbohydrates (WSC) and lignocelluloses contents of different treatments. The relative proportions of Ruminococcus flavefaciens and Fibrobacter succinogenes, carboxymethyl-ocellulose and β-glycosidase activities, DM-D, NDF-D, and VFA production of in vitro incubation was higher (P<0.05) for silages inoculated with LP and EF than those of the control silage. Silage inoculated with LP showed the lowest (P<0.05) CH₄ production per unit yield of VFA, which was positively corresponded to the lowest (P<0.05) ratio of acetate to propionate. In summary, the ensiling fermentation quality and subsequent utilization of corn stover silage were efficiently improved by inoculated with L. plantarum.

Key words: corn stover , in vitro digestibility , lactic acid bacteria , silage

. [J]. Journal of Integrative Agriculture, 2020, 19(3): 838-847.

GUO Gang, SHEN Chen, LIU Qiang, ZHANG Shuan-lin, SHAO Tao, WANG Cong, WANG Yong-xin, XU Qing-fang, HUO Wen-jie.

The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage

[J]. Journal of Integrative Agriculture, 2020, 19(3): 838-847.

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The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage

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