Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (3): 759-767.DOI: 10.1016/S2095-3119(19)62741-3

所属专题: 植物细菌真菌合辑Plant Bacteria/Fungus

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  • 收稿日期:2018-11-29 出版日期:2020-03-01 发布日期:2020-03-04

Regulation of the phytotoxic response of Arabidopsis thaliana to the Fusarium mycotoxin deoxynivalenol

WANG Yan1, 3, YAN Hao2, WANG Qi3, ZHENG Ran3, XIA Kai4, LIU Yang  
  

  1. 1 Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, P.R.China
    2 Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, P.R.China
    3 Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.China
    4 Beijing Key Laboratory of the Innovative Development of Functional Staple and the Nutritional Intervention for Chronic Disease, China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, P.R.China
  • Received:2018-11-29 Online:2020-03-01 Published:2020-03-04
  • Contact: Correspondence LIU Yang, Tel: +86-10-62819472, E-mail: liuyang01@caas.cn; XIA Kai, E-mail: xiakaiphd@126.com
  • About author:WANG Yan, E-mail: wangyan062006@163.com;
  • Supported by:
    The study was supported by the Beijing Municipal Natural Science Foundation of China (5154034), the National Science Foundation of China (31601577), the National Key Research and Development Program of China (2017YFC1600900), the Central Public-interest Scientific Institution Basal Research Fund, and the fund of Beijing Key Laboratory of the Innovative Development of Functional Staple and the Nutritional Intervention for Chronic Disease, China.

Abstract:

Phytopathogenic fungi, such as Fusarium spp., synthesize trichothecene family phytotoxins.  The type B trichothecene, namely deoxynivalenol (DON), is highly prevalent in small-grain cereals, such as wheat, corn and barley.  DON is thought to be a virulence factor allowing plant infections and has an elicitor activity.  We used the model plant Arabidopsis thaliana to evaluate the phytotoxic effects of DON in host plants.  The growth of A. thaliana on media was significantly inhibited by DON.  Moreover, DON induced cell death in detached leaves was observed by trypan blue staining.  This is consistent with the phenomenon of organelle changes observed at the ultrastructural level.  In our study, DON exposure stimulated oxidative bursts in the leaves, resulting in the concomitant down-regulation of antioxidant enzyme defense responses and up-regulation of lipid peroxidation.  In addition, a real-time PCR analysis revealed that the DON treatment rapidly induced the transcription of defense genes, like AtrbohC and AtrbohD, and up-regulated the transcriptional level of the ascorbic acid peroxidase gene.  These results suggested that DON phytotoxicity might result from reactive oxygen species pathways, and that DON production by the plant pathogen Fusarium graminearum can act as an elicitor influencing plant cell fate.
 

Key words: deoxynivalenol ,  Arabidopsis thaliana ,  cell death ,  oxidative stress