Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (3): 768-774.DOI: 10.1016/S2095-3119(19)62677-8

所属专题： 植物病理合辑Plant Protection—Plant Pathology； 植物病毒合辑Plant Virus

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  • 收稿日期:2019-01-14 出版日期:2020-03-01 发布日期:2020-03-04

Prevalence and genetic diversity of grapevine fabavirus isolates from different grapevine cultivars and regions in China

FAN Xu-dong, ZHANG meng-yan, ZHANG Zun-ping, REN Fang, HU Guo-jun, DONG Ya-feng
  

  1. National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, P.R.China
  • Received:2019-01-14 Online:2020-03-01 Published:2020-03-04
  • Contact: Correspondence DONG Ya-feng, Tel: +86-429-3598279, E-mail: yfdong@163.com
  • About author:FAN Xu-dong, E-mail: fanxudong@caas.cn;
  • Supported by:
    This study was supported by the National Key R&D Program of China (2018YFD0201301), the earmarked fund for the China Agriculture Research System (CARS-29-bc-1) and the Fundamental Research Funds for Central Non-profit Scientific Institutions, China.

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Abstract:

A total of 288 grapevine samples of 61 different grapevine cultivars, collected from 22 provinces and regions, were analyzed by reverse transcription-PCR (RT-PCR) for the presence of grapevine fabavirus (GFabV).  PCR detection results showed the incidences of GFabV were 12.8% (30/235) and 48.1% (25/52) in the asymptomatic and symptomatic vines, respectively.  The genetic diversity of GFabV isolates was analyzed based on partial nucleotide and encoded amino acid sequences of the RNA1 and RNA2 polyprotein genes.  Phylogenetic analyses of the RNA1 and RNA2 gene sequences divided the GFabV isolates into five well-defined groups.  Groups 1, 2, and 4 comprised only Chinese isolates.  This article represents the first report for the prevalence and genetic diversity of GFabV in grapevines grown in China.

Key words: GFabV ,  RT-PCR ,  polyprotein gene ,  phylogenetic analysis