Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (11): 2784-2791.DOI: 10.1016/S2095-3119(20)63185-9

所属专题： 动物医学合辑Veterninary Medicine

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  • 收稿日期:2019-10-11 出版日期:2020-11-01 发布日期:2020-10-15

Detection of antimicrobial resistance and virulence-related genes in Streptococcus uberis and Streptococcus parauberis isolated from clinical bovine mastitis cases in northwestern China

ZHANG Hang, YANG Feng, LI Xin-pu, LUO Jin-yin, WANG Ling, ZHOU Yu-long, YAN Yong, WANG Xu-rong, LI Hong-sheng   

  1. Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences/Key Lab of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs/Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine, Lanzhou 730050, P.R.China
  • Received:2019-10-11 Online:2020-11-01 Published:2020-10-15
  • Contact: Correspondence WANG Xu-rong, Tel: +86-931-2112161, Fax: +86-931-2114180, E-mail: wxurong@126.com; LI Hong-sheng, Tel: +86-931-2164183, Fax: +86-931-2114180, E-mail: lihsheng@sina.com
  • About author:ZHANG Hang, E-mail: zh8977@foxmail.com, Mobile: +86-16602725014;
  • Supported by:
    This study was supported by the National Key Research and Development Project of China (2017YFD0502200), the National Natural Science Foundation of China (31802232), and the International Science and Technology Cooperation Project of Gansu Province, China (17YF1WA169).

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Abstract:

The objectives of this study were to investigate antimicrobial resistance of Streptococcus uberis and Streptococcus parauberis isolated from cows with bovine clinical mastitis in China and to examine the distribution of resistance- and virulence-related gene patterns.  Antimicrobial susceptibility was determined by the E-test.  Genes encoding antimicrobial resistance and invasiveness factors were examined by PCR.  A total of 27 strains were obtained from 326 mastitis milk samples.  Streptococcus parauberis isolates (n=11) showed high resistance to erythromycin (90.9%), followed by tetracycline (45.5%), chloramphenicol (36.4%) and clindamycin (27.3%).  Streptococcus uberis isolates (n=16) were highly resistant to tetracycline (81.3%) and clindamycin (62.5%).  Both species were susceptible to ampicillin.  The most prevalent resistance gene in S. uberis was tetM (80.0%), followed by blaZ (62.5%) and ermB (62.5%).  However, tetM, blaZ, and ermB genes were only found in 27.3, 45.5, and 27.3%, respectively, of S. parauberis.  In addition, all of the isolates carried at least one selected virulence-related gene.  The most prevalent virulence-associated gene pattern in the current study was sua+pauA/skc+gapC+hasC detected in 22.2% of the strains.  One S. uberis strain carried 7 virulence-associated genes and belonged to the sua+pauA/skc+gapC+cfu+hasA+hasB+hasC pattern.  More than 59.3% of analysed strains carried 4 to 7 virulence-related genes.  Our findings demonstrated that S. parauberis and S. uberis isolated from clinical bovine mastitis cases in China exhibited diverse molecular ecology, and that the strains were highly resistant to antibiotics commonly used in the dairy cow industry.  The data obtained in the current study contribute to a better understanding of the pathogenesis of bacteria in mastitis caused by these pathogens, and the findings are relevant to the development of multivalent vaccines and targeted prevention procedures.

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