Aggregate-associated changes in nutrient properties, microbial community and functions in a greenhouse vegetable field based on an eight-year fertilization experiment of China

doi:10.1016/S2095-3119(20)63269-5

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (10): 2530-2548.DOI: 10.1016/S2095-3119(20)63269-5

所属专题：农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe

收稿日期:2020-02-10 出版日期:2020-10-01 发布日期:2020-09-08

Aggregate-associated changes in nutrient properties, microbial community and functions in a greenhouse vegetable field based on an eight-year fertilization experiment of China

LUAN Hao-an^{1, 2}, GAO Wei³, TANG Ji-wei¹, LI Ruo-nan⁴, LI Ming-yue³, ZHANG Huai-zhi¹, CHEN Xin-ping², Dainius MASILIUNAS⁵, HUANG Shao-wen¹

¹ Institute of Agricultural Resources and Regional Planning/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
² Center for Resources, Environment and Food Security, China Agricultural University, Beijing 100193, P.R.China
³ Tianjin Institute of Agricultural Resources and Environment, Tianjin 300192, P.R.China
⁴ Institute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, P.R.China
⁵ Laboratory of Geo-information Science and Remote Sensing, Wageningen University, Wageningen 476700, The Netherlands

Received:2020-02-10 Online:2020-10-01 Published:2020-09-08
Contact: Correspondence HUANG Shao-wen, Tel: +86-10-82108662, E-mail: huangshaowen @caas.cn; TANG Ji-wei, Tel: +86-10-82105027, E-mail: tang-jiwei@163.com
About author:LUAN Hao-an, E-mail: luanhaoan@163.com;
Supported by:
The authors sincerely acknowledge the financial support provided by the earmarked fund for China Agriculture Research System (CARS-23-B02), the National Key Research and Development Program of China (2016YFD0201001), and the Key Research and Development Program of Shandong Province, China (2017CXGC0206).

摘要/Abstract

Abstract:

Soil aggregation, microbial community, and functions (i.e., extracellular enzyme activities; EEAs) are critical factors affecting soil C dynamics and nutrient cycling. We assessed soil aggregate distribution, stability, nutrients, and microbial characteristics within >2, 0.25–2, 0.053–0.25, and <0.053 mm aggregates, based on an eight-year field experiment in a greenhouse vegetable field in China. The field experiment includes four treatments: 100% N fertilizer (CF), 50% substitution of N fertilizer with manure (M), straw (S), and manure plus straw (MS). The amounts of nutrient (N, P₂O₅, and K₂O) input were equal in each treatment. Results showed higher values of mean weight diameter in organic-amended soils (M, MS, and S, 2.43–2.97) vs. CF-amended soils (1.99). Relative to CF treatment, organic amendments had positive effects on nutrient (i.e., available N, P, and soil organic C (SOC)) conditions, microbial (e.g., bacterial and fungal) growth, and EEAs in the >0.053 mm aggregates, but not in the <0.053 mm aggregates. The 0.25–0.053 mm aggregates exhibited better nutrient conditions and hydrolytic activity, while the <0.053 mm aggregates had poor nutrient conditions and higher oxidative activity among aggregates, per SOC, available N, available P, and a series of enzyme activities. These results indicated that the 0.25–0.053 mm (<0.053 mm) aggregates provide suitable microhabitats for hydrolytic (oxidative) activity. Interestingly, we found that hydrolytic and oxidative activities were mainly impacted by fertilization (58.5%, P<0.01) and aggregate fractions (50.5%, P<0.01), respectively. The hydrolytic and oxidative activities were significantly (P<0.01) associated with nutrients (SOC and available N) and pH, electrical conductivity, respectively. Furthermore, SOC, available N, and available P closely (P<0.05) affected microbial communities within >0.25, 0.25–0.053, and <0.053 mm aggregates, respectively. These findings provide several insights into microbial characteristics within aggregates under different fertilization modes in the greenhouse vegetable production system in China.

Key words: fertilization , soil aggregate distribution , microbial characteristics

. [J]. Journal of Integrative Agriculture, 2020, 19(10): 2530-2548.

LUAN Hao-an, GAO Wei, TANG Ji-wei, LI Ruo-nan, LI Ming-yue, ZHANG Huai-zhi, CHEN Xin-ping, Dainius MASILIUNAS, HUANG Shao-wen. Aggregate-associated changes in nutrient properties, microbial community and functions in a greenhouse vegetable field based on an eight-year fertilization experiment of China[J]. Journal of Integrative Agriculture, 2020, 19(10): 2530-2548.

参考文献

Abiven S, Menasseri S, Angers D A, Leterme P. 2007. Dynamics of aggregate stability and biological binding agents during decomposition of organic materials. European Journal of Soil Science, 58, 239–247.
Abiven S, Menasseri S, Chenu C. 2009. The effects of organic inputs over time on soil aggregate stability - A literature analysis. Soil Biology & Biochemistry, 41, 1–12.
Agegnehu G, Nelson P N, Bird M I. 2016. Crop yield, plant nutrient uptake and soil physicochemical properties under organic soil amendments and nitrogen fertilization on Nitisols. Soil & Tillage Research, 160, 1–13.
Allison S D, Jastrow J D. 2006. Activities of extracellular enzymes in physically isolated fractions of restored grassland soils. Soil Biology & Biochemistry, 38, 3245–3256.
Arienzo M, Christen E M, Quayle W, Kumar A. 2009. A review of the fate of potassium in the soil–plant system after land application of wastewaters. Journal of Hazardous Materials, 164, 415–422.
Awad Y M, Lee S S, Kim K H, Ok Y S. 2018. Carbon and nitrogen mineralization and enzyme activities in soil aggregate-size classes: Effects of biochar oyster shells and polymers. Chemosphere, 198, 40–48.
Ayoubi S, Karchegani P M, Mosaddeghi M R, Naser H. 2012. Soil aggregation and organic carbon as affected by topography and land use change in western Iran. Soil & Tillage Research, 121, 18–26.
Bach E M, Baer S G, Meyer C K, Six J. 2010. Soil texture affects soil microbial and structural recovery during grassland restoration. Soil Biology & Biochemistry, 42, 2182–2191.
Bach E M, Williams R J, Hargreaves S K, Yang F, Hofmockel K S. 2018. Greatest soil microbial diversity found in micro-habitats. Soil Biology & Biochemistry, 118, 217–226.
Balser T C, Kirchner J W, Firestone M K. 2002. Methodological variability in microbial community level physiological profiles. Soil Science Society of America Journal, 66, 519–523.
Banerjee J, Bora S, Thrall P H, Richardson A E. 2016. Soil C and N as causal factors of spatial variation in extracellular enzyme activity across grassland-woodland ecotones. Applied Soil Ecology, 105, 1–8.
Bei S, Zhang Y, Li T, Christie P, Li X, Zhang J. 2018. Response of the soil microbial community to different fertilizer inputs in a wheat-maize rotation on a calcareous soil. Agriculture, Ecosystems and Environment, 260, 58–69.
Bell C W, Fricks B E, Rocca J D, Steinweg J M, McMahon S K, Wallenstein M D. 2013. High-throughput fluorometric measurement of potential soil extracellular enzyme activities. Jove-Journal of Visualized Experiments, 81, e50961.
Burns R G, Deforest J L, Marxsen J, Sinsabaugh R L, Stromberger M E, Wallenstein M D, Weintraub M N, Zoppini A. 2013. Soil enzymes in a changing environment: Current knowledge and future directions. Soil Biology & Biochemistry, 58, 216–234.
Chen J, He F, Zhang X, Sun X, Zheng J, Zheng J. 2014. Heavy metal pollution decreases microbial abundance, diversity and activity within particle-size fractions of a paddy soil. FEMS Microbiology Ecology, 87, 164–181.
Chen X, Li Z, Liu M, Jiang C, Che Y. 2015. Microbial community and functional diversity associated with different aggregate fractions of a paddy soil fertilized with organic manure and/or NPK fertilizer for 20 years. Journal of Soils and Sediments, 15, 292–301.
Chung H, Grove J H, Six J. 2008. Indications for soil carbon saturation in a temperate agroecosystem, Soil Science Society of America Journal, 72, 1132–1139,
Davinic M, Fultz L M, Acosta-Martinez V, Calderón F J, Cox S B, Dowd S E, Allen V G, Zak J C, Moore-Kucera J. 2012. Pyrosequencing and mid-infrared spectroscopy reveal distinct aggregate stratification of soil bacterial communities and organic matter composition. Soil Biology & Biochemistry, 46, 63–72.
Dong W, Zhang X, Dai X, Fu X, Yang F, Liu X, Sun X, Wen X, Schaeffer S. 2014. Changes in soil microbial community composition in response to fertilization of paddy soils in subtropical China. Applied Soil Ecology, 84, 140–147.
Fierer N, Schimel J P, Holden P A. 2003. Variations in microbial community composition through two soil depth profiles. Soil Biology & Biochemistry, 35, 167–176.
Frostegård Å, Tunlid A, Bååth E. 2011. Use and misuse of PLFA measurements in soils. Soil Biology & Biochemistry, 43, 1621–1625.
Grandy A S, Neff J C. 2008. Molecular C dynamics downstream: The biochemical decomposition sequence and its impact on soil organic matter structure and function. Science of the Total Environment, 404, 271–307.
Guan S, Dou S, Chen G, Wang G, Zhuang J. 2015. Isotopic characterization of sequestration and transformation of plant residue carbon in relation to soil aggregation dynamics. Applied Soil Ecology, 96, 18–24.
Guo Z, Zhang L, Yang W, Hua L, Cai, C. 2019. Aggregate stability under long-term fertilization practices: The case of eroded Ultisols of South-Central China. Sustainability, 11, 1169.
Hu W, Zhang Y, Huang B, Teng Y. 2017. Soil environmental quality in greenhouse vegetable production systems in eastern China: Current status and management strategies. Chemosphere, 170, 183–195.
Huang S, Peng X, Huang Q, Zhang W. 2010. Soil aggregation and organic carbon fractions affected by long-term fertilization in a red soil of subtropical China. Geoderma, 154, 364–369.
Huang S, Tang J, Li C, Zhang H, Yuan S. 2017. Reducing potential of chemical fertilizers and scientific fertilization countermeasure in vegetable production in China. Journal of Plant Nutrition and Fertilizers, 23, 1480–1493.
Jian S, Li J, Chen J, Wang G, Mayes M A, Dzantor K E, Hui D, Luo Y. 2016. Soil extracellular enzyme activities soil carbon and nitrogen storage under nitrogen fertilization: A meta-analysis. Soil Biology & Biochemistry, 101, 32–43.
Jiang X, Wright A L, Wang J, Li Z. 2011. Long-term tillage effects on the distribution patterns of microbial biomass and activities within soil aggregates. Catena, 87, 276–280.
Jiang Y, Sun B, Jin C, Wang F. 2013. Soil aggregate stratification of nematodes and microbial communities affects the metabolic quotient in an acid soil. Soil Biology & Biochemistry, 60, 1–9.
Jing Y, Wang Y, Liu S, Zhang X, Wang Q, Liu K, Yin Y, Deng J. 2019. Interactive effects of soil warming, throughfall reduction, and root exclusion on soil microbial community and residues in warm-temperate oak forests. Applied Soil Ecology, 142, 52–58.
Keeler B L, Hobbie S E, Kellogg L E. 2009. Effects of long-term nitrogen addition on microbial enzyme activity in eight forested and grassland sites: implications for litter and soil organic matter decomposition. Ecosystems, 12, 1–15.
Kim H, Nunan N, Dechesne A, Juarez S, Grundmann G. 2015. The spatial distribution of exoenzyme activities across the soil micro-landscape as measured in micro- and macro-aggregates and ecosystem processes. Soil Biology & Biochemistry, 91, 258–267.
Kong A Y Y, Scow K M, Córdova-Kreylos A L, Holmes W E, Six J. 2011. Microbial community composition and carbon cycling within soil microenvironments of conventional low-input and organic cropping systems. Soil Biology & Biochemistry, 43, 20–30.
Kuzyakov Y, Blagodatskaya E. 2015. Microbial hotspots and hot moments in soil: Concept & review. Soil Biology & Biochemistry, 83, 184–199.
Lazcano C, Gómez-Brandón M, Revilla P, Domínguez J. 2013. Short-term effects of organic and inorganic fertilizers on soil microbial community structure and function. Biology and Fertility of Soils, 49, 723–733.
Li C, Yan K, Tang L, Jia Z, Li Y. 2014. Change in deep soil microbial communities due to long-term fertilization. Soil Biology & Biochemistry, 75, 264–272.
Li J, Cooper J M, Lin Z A, Li Y, Yang X, Zhao B. 2015. Soil microbial community structure and function are significantly affected by long-term organic and mineral fertilization regimes in the North China Plain. Applied Soil Ecology, 96, 75–87.
Li N, Yao S, Qiao Y, Zou W, You M, Han X, Zhang B. 2015. Separation of soil microbial community structure by aggregate size to a large extent under agricultural practices during early pedogenesis of a Mollisol. Applied Soil Ecology, 88, 9–20.
Li S, Lei Y, Zhang Y, Liu J, Shi X, Jia H, Wang C, Chen F, Chu Q. 2019. Rational trade-offs between yield increase and fertilizer inputs are essential for sustainable intensification: A case study in wheat–maize cropping systems in China. Science of the Total Environment, 679, 328–336.
Li S, Li Q, Wang C, Li B, Gao X, Li Y, Wu D. 2019. Spatial variability of soil bulk density and its controlling factors in an agricultural intensive area of Chengdu Plain, Southwest China. Journal of Integrative Agriculture, 18, 290–300.
Li S, Zhang S, Pu Y, Li T, Xu X, Jia Y, Deng O, Guo G. 2016. Dynamics of soil labile organic carbon fractions and C-cycle enzyme activities under straw mulch in Chengdu Plain. Soil & Tillage Research, 155, 289–297.
Liao H, Zhang Y, Zuo Q, Du B, Chen W, Wei D, Huang Q. 2018. Contrasting responses of bacterial and fungal communities to aggregate-size fractions and long-term fertilizations in soils of northeastern China. Science of the Total Environment, 635, 784–792.
Liao Y, Zheng S, Nie J, Xie J, Lu Y, Qin X. 2013. Long-term effect of fertilizer and rice straw on mineral composition and potassium adsorption in a reddish paddy soil. Journal of Integrative Agriculture, 12, 694–710.
Lin Y, Ye G, Kuzyakov Y, Liu D, Fan J, Ding W. 2019. Long-term manure application increases soil organic matter and aggregation and alters microbial community structure and keystone taxa. Soil Biology & Biochemistry, 134, 187–196.
Liu H, Zhang J, Ai Z, Wu Y, Xu H, Li Q, Xue S, Liu G. 2018. 16-Year fertilization changes the dynamics of soil oxidizable organic carbon fractions and the stability of soil organic carbon in soybean–corn agroecosystem. Agriculture, Ecosystems and Environment, 265, 320–330.
Liu K, Han T, Huang J, Huang Q, Li D, Hu Z, Yu X, Muhammad Q, Ahmed W, Hu H, Zhang H. 2019. Response of soil aggregate-associated potassium to long-term fertilization in red soil. Geoderma, 352, 160–170.
Liu M, Hu F, Chen X, Huang Q, Jiao J, Zhang B, Li H. 2009. Organic amendments with reduced chemical fertilizer promote soil microbial development and nutrient availability in a subtropical paddy field: The influence of quantity, type and application time of organic amendments. Applied Soil Ecology, 42, 166–175.
Liu X, Zhou F, Hu G, Shao S, He H, Zhang W, Zhang X, Li L. 2019. Dynamic contribution of microbial residues to soil organic matter accumulation influenced by maize straw mulching. Geoderma, 333, 35–42.
Liu Y, Li X, Shen Q, Xu Y. 2013. Enzyme activity in water-stable soil aggregates as affected by long-term application of organic manure and chemical fertiliser. Pedosphere, 23, 111–119.
Liu Z, Chen X, Jing Y, Li Q, Zhang J, Huang Q. 2014. Effects of biochar amendment on rapeseed and sweet potato yields and water stable aggregate in upland red soil. Catena, 123, 45–51.
Luan H, Gao W, Huang S, Tang J, Li M, Zhang H, Chen X. 2019. Partial substitution of chemical fertilizer with organic amendments affects soil organic carbon composition and stability in a greenhouse vegetable production system. Soil & Tillage Research, 191, 185–196.
Luo L, Meng H, Gu J. 2017. Microbial extracellular enzymes in biogeochemical cycling of ecosystems. Journal of Environmental Management, 197, 539–549.
Ma X, Liu M, Li Z. 2016. Shifts in microbial biomass and community composition in subtropical paddy soils under a gradient of manure amendment. Biology and Fertility of Soils, 52, 775–787.
Mebius L J. 1960. A rapid method for the determination of organic carbon in soil. Analytica Chimica Acta, 22, 120–124.
Moeskops B, Buchan D, Beneden S V, Fievez V, Sleutel S, Gasper M S, D’Hose T, Neve S D. 2012. The impact of exogenous organic matter on SOM contents and microbial soil quality. Pedobiologia, 55, 175–184.
Moeskops B, Sukristiyonubowo B D, Sleutel S, Herawaty L, Husen E, Saraswati R, Setyorini D, Neve S D. 2010. Soil microbial communities and activities under intensive organic and conventional vegetable farming in West Java Indonesia. Applied Soil Ecology, 45, 112–120.
Muhammad Q, Huang J, Waqas A, Li D, Liu S, Zhang L, Cai A, Liu L, Xu Y, Gao J, Zhang H. 2020. Yield sustainability, soil organic carbon sequestration and nutrients balance under long-term combined application of manure and inorganic fertilizers in acidic paddy soil. Soil & Tillage Research, 198, 104569.
Murugan R, Djukic I, Keiblinger K, Zehetner F, Bierbaumer M, Zechmeister-Bolternstern S, Joergernsen R G. 2019. Spatial distribution of microbial biomass and residues across soil aggregate fractions at different elevations in the Central Austrian Alps. Geoderma, 339, 1–8.
Nie M, Pendall E, Bell C, Wallenstein M D. 2014. Soil aggregate size distribution mediates microbial climate change feedbacks. Soil Biology & Biochemistry, 68, 357–365.
Olsen S R, Olsen L E. 1982. Sommers Methods of Soil Analysis. 9th ed. American Society of Agronomy and Soil Science Society of America, Madison, WI, USA.
Regelink I C, Stoof C R, Rousseva S, Weng L, Lair G J, Kram P, Nikolaidis N P, Kercheva M, Banwart S, Comans R N J. 2015. Linkages between aggregate formation porosity and soil chemical properties. Geoderma, 247–248, 24–37.
Rong Q, Li R, Huang S, Tang J, Zhang Y, Wang L. 2018. Soil microbial characteristics and yield response to partial substitution of chemical fertilizer with organic amendments in greenhouse vegetable production. Journal of Integrative Agriculture, 17, 1432–1444.
Rousk J, Brookes P C, Bååth E. 2010. The microbial PLFA composition as affected by pH in an arable soil. Soil Biology & Biochemistry, 42, 516–520.
Schnecker J, Wild B, Takriti M, Eloy A R J, Gentsch N, Gittel A, Hofer A, Klaus K, Knoltsch A, Lashchinskiy N, Mikutta R, Richter A. 2015. Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in western Siberia. Soil Biology & Biochemistry, 83, 106–115.
Simonetti G, Francioso O, Ferro N D, Nardi S, Berti A, Morari F. 2017. Soil porosity in physically separated fractions and its role in SOC protection. Journal of Soils and Sediments, 17, 70–84.
Sinsabaugh R L. 2010. Phenol oxidase peroxidase and organic matter dynamics of soil. Soil Biology & Biochemistry, 42, 391–404.
Six J, Bossuyt H, Degryze S, Denef K. 2004. A history of research on the link between (micro)aggregates soil biota and soil organic matter dynamics. Soil & Tillage Research, 79, 7–31.
Tautges N E, Sullivan T S, Reardon C L, Burke I C. 2016. Soil microbial diversity and activity linked to crop yield and quality in a dryland organic wheat production system. Applied Soil Ecology, 108, 258–268.
Totsche K U, Amelung W, Gerzabek M H, Guggenberger G, Klumpp E, Knief C, Lehndorff E, Mikutta R, Peth S, Prechtel A, Ray N, Kogel-Knabner I. 2018. Microaggregates in soils. Journal of Plant Nutrition and Soil Science, 181, 104–136.
Upton R N, Bach E M, Hofmockel K S. 2019. Spatio-temporal microbial community dynamics within soil aggregates. Soil Biology & Biochemistry, 132, 58–68.
Wang C, Lu X, Mori T, Mao Q, Zhou K, Zhou G, Nie G, Mo J. 2018. Responses of soil microbial community to continuous experimental nitrogen additions for 13 years in a nitrogen-rich tropical forest. Soil Biology & Biochemistry, 121, 103–112.
Wang H, Guan D, Zhang R, Chen Y, Hu Y, Xiao L. 2014. Soil aggregates and organic carbon affected by the land use change from rice paddy to vegetable field. Ecological Engineering, 70, 206–211.
Wang H, Nie Y, Butterly C R, Wang L, Chen Q, Tian W, Song B, Xi Y, Wang Y. 2017. Fertilization alters microbial community composition and functional patterns by changing the chemical nature of soil organic carbon: A field study in a Halosol. Geoderma, 292, 17–24.
Wang R, Dorodnikov M, Yang S, Zhang Y, Filley T R, Turco R F, Zhang Y, Xu Z, Li H, Jiang Y. 2015. Responses of enzymatic activities within soil aggregates to 9-year nitrogen and water addition in a semi-arid grassland. Soil Biology & Biochemistry, 81, 159–167.
Wang S, Li T, Zheng Z. 2017. Distribution of microbial biomass and activity within soil aggregates as affected by tea plantation age. Catena, 153, 1–8.
Wang S, Li T, Zheng Z. 2018a. Response of soil aggregate-associated microbial and nematode communities to tea plantation age. Catena, 171, 475–484.
Wang S, Li T, Zheng Z. 2018b. Tea plantation age effects on soil aggregate-associated carbon and nitrogen in the hilly region of western Sichuan China. Soil & Tillage Research, 180, 91–98.
Wang Y, Hu N, Ge T, Kuzyakov Y, Wang Z, Li Z, Tang Z, Chen Y, Wu C, Lou Y. 2017. Soil aggregation regulates distributions of carbon microbial community and enzyme activities after 23-year manure amendment. Applied Soil Ecology, 111, 65–72.
Xie H, Li, J, Zhang B, Wang L, Wang J, He H, Zhang X. 2015. Long-term manure amendments reduced soil aggregate stability via redistribution of the glomalin-related soil protein in macroaggregates. Scientific Reports, 5, 14687.
Yan X, Zhou H, Zhu Q, Wang X, Zhang Y, Yu X, Peng X. 2013. Carbon sequestration efficiency in paddy soil and upland soil under long-term fertilization in southern China. Soil & Tillage Research, 130, 42–51.
Yang L, Huang B, Hu W, Chen Y, Mao M, Yao L. 2014. The impact of greenhouse vegetable farming duration and soil types on phytoavailability of heavy metals and their health risk in eastern China. Chemosphere, 103, 121–130.
Yao Y, Ge N, Yu S, Wei X, Wang X, Jin J, Liu X, Shao M, Wei Y, Kang L. 2019. Response of aggregate associated organic carbon nitrogen and phosphorous to re-vegetation in agro-pastoral ecotone of northern China. Geoderma, 341, 172–180.
Yu H, Ding W, Chen Z, Zhang H, Luo J, Bolan N. 2015. Accumulation of organic C components in soil and aggregates. Scientific Reports, 5, 13804.
Yu H, Ding W, Luo J, Geng R, Cai Z. 2012a. Long-term application of organic manure and mineral fertilizers on aggregation and aggregate-associated carbon in a sandy loam soil. Soil & Tillage Research, 124, 170–177.
Yu H, Ding W, Luo J, Geng R, Ghani, A, Cai Z. 2012b. Effects of long-term compost and fertilizer application on stability of aggregate-associated organic carbon in an intensively cultivated sandy loam soil. Biology and Fertility of Soils, 48, 325–336.
Yu L, Yu M, Lu X, Tang C, Liu X, Brookes P C, Xu J. 2018. Combined application of biochar and nitrogen fertilizer benefits nitrogen retention in the rhizosphere of soybean by increasing microbial biomass but not altering microbial community structure. Science of the Total Environment, 640–641, 1221–1230.
Zhang H, Huang B, Dong L, Hu W, Akhtar M S, Qu M. 2017. Accumulation sources and health risks of trace metals in elevated geochemical background soils used for greenhouse vegetable production in southwestern China. Ecotoxicology and Environmental Safety, 137, 233–239.
Zhang J, Zhuang M, Shan N, Zhao Q, Li H, Wang L. 2019. Substituting organic manure for compound fertilizer increases yield and decreases NH3 and N2O emissions in an intensive vegetable production systems. Science of the Total Environment, 670, 1184–1189.
Zhang Q, Liang G, Zhou W, Sun J, Wang X, He P. 2016. Fatty-acid profiles and enzyme activities in soil particle-size fractions under long-term fertilization. Soil Science Society of America Journal, 80, 97–111.
Zhang Q, Shamsi I H, Xu D, Wang G, Lin X, Jilani G, Hussain N, Chaudhry A N. 2012. Chemical fertilizer and organic manure inputs in soil exhibit a vice versa pattern of microbial community structure. Applied Soil Ecology, 57, 1–8.
Zhang S, Li Q, Lv Y, Zhang L, Liang W. 2013. Contributions of soil biota to C sequestration varied with aggregate fractions under different tillage systems. Soil Biology & Biochemistry, 62, 147–156.
Zhang X, Wu X, Zhang S, Xing Y, Wang R, Liang W. 2014. Organic amendment effects on aggregate-associated organic C microbial biomass C and glomalin in agricultural soils. Catena, 123, 188–194.
Zhou H, Fang H, Mooney S J, Peng X. 2016. Effects of long-term inorganic and organic fertilizations on the soil micro and macro structures of rice paddies. Geoderma, 266, 66–74.

Aggregate-associated changes in nutrient properties, microbial community and functions in a greenhouse vegetable field based on an eight-year fertilization experiment of China

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