Biotic and abiotic stress-responsive genes are stimulated to resist drought stress in purple wheat

doi:10.1016/S2095-3119(19)62659-6

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (1): 33-50.DOI: 10.1016/S2095-3119(19)62659-6

所属专题：麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources

收稿日期:2018-09-23 出版日期:2020-01-01 发布日期:2019-12-23

Biotic and abiotic stress-responsive genes are stimulated to resist drought stress in purple wheat

LI Xiao-lan^{1, 3}, LÜ Xiang¹, WANG Xiao-hong¹, PENG Qin², ZHANG Ming-sheng¹, REN Ming-jian²

¹ School of Life Sciences, Guizhou University/State Engineering Technology Institute for Karst Desertification Control/Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang 550025, P.R.China
² School of Agriculture, Guizhou University/Guizhou Sub-Center of National Wheat Improvement Center, Guiyang 550025, P.R.China
³ Special Key Laboratory of Microbial Resources and Drug Development from Higher Education Institution of Guizhou Province/Research Center for Medicine and Biology, Zunyi Medical University, Zunyi 563006, P.R.China

Received:2018-09-23 Online:2020-01-01 Published:2019-12-23
Contact: Correspondence REN Ming-jian, Tel: +86-851-83855894, E-mail: rmj72@163.com
About author: LI Xiao-lan, Tel: +86-851-28642444, E-mail: lixiaolanl@163.com;
Supported by:
This study was supported by the grants from the National Key R&D Program of China (2017YFD0100901-4 and 2016YFC0502604), the National Natural Science Foundation of China (31660390), the Major Special Project of Science and Technology Program in Guizhou, China (2017-5411-06 and 2017-5788), the Construction Project of State Engineering Technology Institute for Karst Desertification Control, China (2012FU125X13), the Innovation Talents Team Construction of Science and Technology in Guizhou, China (2016-5624), the Major Research Project of Innovation Group in Guizhou, China (2016-023), and the Graduate Innovation Fund of Guizhou University, China (2017025), and the Science and Technology Project in Guizhou, China (2019-4246).

摘要/Abstract

Abstract:

Triticum aestivum L. cv. Guizi 1 (GZ1) is a drought-tolerant local purple wheat cultivar. It is not clear how purple wheat resists drought stress, but it could be related to anthocyanin biosynthesis. In this study, transcriptome data from drought-treated samples and controls were compared. Drought slightly reduced the anthocyanin, protein and starch contents of GZ1 grains and significantly reduced the grain weight. Under drought stress, 16 682 transcripts were reduced, 27 766 differentially expressed genes (DEGs) were identified, and 379 DEGs, including DREBs, were related to defense response. The defense-response genes included response to water deprivation, reactive oxygen, bacteria, fungi, etc. Most of the structural and regulatory genes in anthocyanin biosynthesis were downregulated, with only TaDFR, TaOMT, Ta5,3GT, and TaMYB-4B1 being upregulated. TaCHS, TaF3H, TaCHI, Ta4CL, and TaF3’H are involved in responses to UV, hormones, and stimulus. TaCHS-2D1, TaDFR-2D2, TaDFR-7D, TaOMT-5A, Ta5,3GT-1B1, Ta5,3GT-3A, and Ta5,3GT-7B1 connect anthocyanin biosynthesis with other pathways, and their interacting proteins are involved in primary metabolism, genetic regulation, growth and development, and defense responses. There is further speculation about the defense-responsive network in purple wheat. The results indicated that biotic and abiotic stress-responsive genes were stimulated to resist drought stress in purple wheat GZ1, and anthocyanin biosynthesis also participated in the drought defense response through several structural genes.

Key words: transcriptome , purple wheat, drought , anthocyanin , differentially expressed genes , defense response , stress

. [J]. Journal of Integrative Agriculture, 2020, 19(1): 33-50.

LI Xiao-lan, Lü Xiang, WANG Xiao-hong, PENG Qin, ZHANG Ming-sheng, REN Ming-jian . Biotic and abiotic stress-responsive genes are stimulated to resist drought stress in purple wheat[J]. Journal of Integrative Agriculture, 2020, 19(1): 33-50.

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Biotic and abiotic stress-responsive genes are stimulated to resist drought stress in purple wheat

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