Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobacterium-mediated co-transformation in T2 and T3 generations

doi:10.1016/S2095-3119(19)62601-8

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (1): 23-32.DOI: 10.1016/S2095-3119(19)62601-8

所属专题：麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources

收稿日期:2018-09-05 出版日期:2020-01-01 发布日期:2019-12-23

Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobacterium-mediated co-transformation in T₂ and T₃generations

LIU Hui-yun1, 2, WANG Ke2, WANG Jing2, DU Li-pu2, PEI Xin-wu1, YE Xing-guo2

¹Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
² Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2018-09-05 Online:2020-01-01 Published:2019-12-23
Contact: Correspondence PEI Xin-wu, E-mail: peixinwu@caas.cn; YE Xing-guo, E-mail: yexingguo@caas.cn
About author:
Supported by:
The authors acknowledge the Ministry of Agriculture of China for the National Transgenic Research Program (2016ZX08010004) and the Chinese Academy of Agricultural Sciences for the Agricultural Science and Technology Innovation Program (ASTIP-2060302-2-19).

摘要/Abstract

Abstract:

Genetically modified wheat has not been commercially utilized in agriculture largely due to regulatory hurdles associated with traditional transformation methods. Development of marker-free transgenic wheat plants will help to facilitate biosafety evaluation and the eventual environmental release of transgenic wheat varieties. In this study, the marker-free transgenic wheat plants previously obtained by Agrobacterium-mediated co-transformation of double T-DNAs vector were identified by fluorescence in situ hybridization (FISH) in the T₁ generation, and their genetic stability and agronomic traits were analyzed in T₂ and T₃ generations. FISH analysis indicated that the transgene often integrated into a position at the distal region of wheat chromosomes. Furthermore, we show that the GUS transgene was stably inherited in the marker-free transgenic plants in T₁ to T₃ generations. No significant differences in agronomic traits or grain characteristics were observed in T₃generation, with the exception of a small variation in spike length and grains per spike in a few lines. The selection marker of bar gene was not found in the transgenic plants through T₁ to T₃ generations. The results from this investigation lay a solid foundation for the potential application of the marker-free transgenic wheat plants achieved through the co-transformation of double T-DNAs vector by Agrobacterium in agriculture after biosafty evaluation.

Key words: wheat , marker-free transgenic plants , fluorescence in situ hybridization , genetic stability

. [J]. Journal of Integrative Agriculture, 2020, 19(1): 23-32.

LIU Hui-yun, WANG Ke, WANG Jing, DU Li-pu, PEI Xin-wu, YE Xing-guo . Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobacterium-mediated co-transformation in T₂ and T₃generations[J]. Journal of Integrative Agriculture, 2020, 19(1): 23-32.

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Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobacterium-mediated co-transformation in T₂ and T₃generations

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