Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)

doi:10.1016/S2095-3119(15)61133-9

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (4): 840-847.DOI: 10.1016/S2095-3119(15)61133-9

Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)

CHEN Tai-yu^{1, 2}, HOU Ji-xiang¹, LIN Yong-jun¹

¹ National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China
² College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China

收稿日期:2015-04-02 出版日期:2016-04-01 发布日期:2016-04-07
通讯作者: LIN Yong-jun, Tel: +86-27-87281719, E-mail: yongjunlin@mail.hzau.edu.cn
作者简介:CHEN Tai-yu, Tel: +86-27-87280516, E-mail: ctycxd@163.com
基金资助:
This research was funded by the National Program of Transgenic Variety Development of China (2014ZX08001-001) and the China Postdoctoral Science Foundation (2013M531705).

Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)

CHEN Tai-yu^{1, 2}, HOU Ji-xiang¹, LIN Yong-jun¹

¹ National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China
² College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China

Received:2015-04-02 Online:2016-04-01 Published:2016-04-07
Contact: LIN Yong-jun, Tel: +86-27-87281719, E-mail: yongjunlin@mail.hzau.edu.cn
About author:CHEN Tai-yu, Tel: +86-27-87280516, E-mail: ctycxd@163.com
Supported by:
This research was funded by the National Program of Transgenic Variety Development of China (2014ZX08001-001) and the China Postdoctoral Science Foundation (2013M531705).

摘要/Abstract

摘要： RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome of Nephotettix cincticeps (Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81 225 transcripts were obtained with the length from 150 bp to about 4.2 kb. Almost all the genes related to the RNAi core pathway were proved to be present in N. cincticeps transcriptome. Two transcripts that respectively encode a systemic interference defective (SID) were identified in our database, indicating that the systematic RNAi pathway can function effectively in N. cincticeps. Our datasets not only supply basic gene information for the studies of gene expression and functions in N. cincticeps, such as the control genes for gene expression analysis, but also provide candidate genes for RNAi pest management, such as the genes that encode P450 monooxygenase, V-ATPase and chitin synthase.

关键词: Nephotettix cincticeps , transcriptome , RNA interference , house-keeping genes

Abstract: RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome of Nephotettix cincticeps (Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81 225 transcripts were obtained with the length from 150 bp to about 4.2 kb. Almost all the genes related to the RNAi core pathway were proved to be present in N. cincticeps transcriptome. Two transcripts that respectively encode a systemic interference defective (SID) were identified in our database, indicating that the systematic RNAi pathway can function effectively in N. cincticeps. Our datasets not only supply basic gene information for the studies of gene expression and functions in N. cincticeps, such as the control genes for gene expression analysis, but also provide candidate genes for RNAi pest management, such as the genes that encode P450 monooxygenase, V-ATPase and chitin synthase.

Key words: Nephotettix cincticeps , transcriptome , RNA interference , house-keeping genes

CHEN Tai-yu, HOU Ji-xiang, LIN Yong-jun. Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)[J]. Journal of Integrative Agriculture, 2016, 15(4): 840-847.

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Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)

Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)

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